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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.10 P.818-838


Low‐intensity pulsed ultrasound ameliorates angiotensin II-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway

Author(s):  Kun ZHAO, Jing ZHANG, Tianhua XU, Chuanxi YANG, Liqing WENG, Tingting WU, Xiaoguang WU, Jiaming MIAO, Xiasheng GUO, Juan TU, Dong ZHANG, Bin ZHOU, Wei SUN, Xiangqing KONG

Affiliation(s):  Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; more

Corresponding email(s):   weisun7919@njmu.edu.cn, bin.zhou@einstein.yu.edu

Key Words:  Low-intensity pulsed ultrasound (LIPUS), Caveolin-1, Cardiac fibrosis, Inflammation, Angiotensin II (AngII)

Kun ZHAO, Jing ZHANG, Tianhua XU, Chuanxi YANG, Liqing WENG, Tingting WU, Xiaoguang WU, Jiaming MIAO, Xiasheng GUO, Juan TU, Dong ZHANG, Bin ZHOU, Wei SUN, Xiangqing KONG. Low‐intensity pulsed ultrasound ameliorates angiotensin II-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway[J]. Journal of Zhejiang University Science B, 2021, 22(10): 818-838.

@article{title="Low‐intensity pulsed ultrasound ameliorates angiotensin II-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway",
author="Kun ZHAO, Jing ZHANG, Tianhua XU, Chuanxi YANG, Liqing WENG, Tingting WU, Xiaoguang WU, Jiaming MIAO, Xiasheng GUO, Juan TU, Dong ZHANG, Bin ZHOU, Wei SUN, Xiangqing KONG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Low‐intensity pulsed ultrasound ameliorates angiotensin II-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway
%A Tianhua XU
%A Chuanxi YANG
%A Liqing WENG
%A Tingting WU
%A Xiaoguang WU
%A Jiaming MIAO
%A Xiasheng GUO
%A Juan TU
%A Wei SUN
%A Xiangqing KONG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 10
%P 818-838
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100130

T1 - Low‐intensity pulsed ultrasound ameliorates angiotensin II-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway
A1 - Kun ZHAO
A1 - Jing ZHANG
A1 - Tianhua XU
A1 - Chuanxi YANG
A1 - Liqing WENG
A1 - Tingting WU
A1 - Xiaoguang WU
A1 - Jiaming MIAO
A1 - Xiasheng GUO
A1 - Juan TU
A1 - Dong ZHANG
A1 - Bin ZHOU
A1 - Wei SUN
A1 - Xiangqing KONG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 10
SP - 818
EP - 838
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100130

ObjectiveCardiac hypertrophy and fibrosis are major pathological manifestations observed in left ventricular remodeling induced by angiotensin II (AngII). low-intensity pulsed ultrasound (LIPUS) has been reported to ameliorate cardiac dysfunction and myocardial fibrosis in myocardial infarction (MI) through mechano-transduction and its downstream pathways. In this study, we aimed to investigate whether LIPUS could exert a protective effect by ameliorating AngII-induced cardiac hypertrophy and fibrosis and if so, to further elucidate the underlying molecular mechanisms.
MethodsWe used AngII to mimic animal and cell culture models of cardiac hypertrophy and fibrosis. LIPUS irradiation was applied in vivo for 20 min every 2 d from one week before mini-pump implantation to four weeks after mini-pump implantation, and in vitro for 20 min on each of two occasions 6 h apart. Cardiac hypertrophy and fibrosis levels were then evaluated by echocardiographic, histopathological, and molecular biological methods.
ResultsOur results showed that LIPUS could ameliorate left ventricular remodeling in vivo and cardiac fibrosis in vitro by reducing AngII-induced release of inflammatory cytokines, but the protective effects on cardiac hypertrophy were limited in vitro. Given that LIPUS increased the expression of caveolin-1 in response to mechanical stimulation, we inhibited caveolin-1 activity with pyrazolopyrimidine 2 (pp2) in vivo and in vitro. LIPUS-induced downregulation of inflammation was reversed and the anti-fibrotic effects of LIPUS were absent.
ConclusionsThese results indicated that LIPUS could ameliorate AngII-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway, providing new insights for the development of novel therapeutic apparatus in clinical practice.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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