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On-line Access: 2021-11-16

Received: 2021-04-20

Revision Accepted: 2021-06-15

Crosschecked: 0000-00-00

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jianwei CHANG

https://orcid.org/0000-0002-1219-360X

Runqiang YANG

https://orcid.org/0000-0002-0191-6315

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.11 P.906-916

http://doi.org/10.1631/jzus.B2100266


Red light enhances folate accumulation in wheat seedlings


Author(s):  Jianwei CHANG, Chong XIE, Pei WANG, Zhenxin GU, Yongbin HAN, Runqiang YANG

Affiliation(s):  College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   yangrq@njau.edu.cn

Key Words:  Wheat, Red light, Light intensity, Folate accumulation


Jianwei CHANG, Chong XIE, Pei WANG, Zhenxin GU, Yongbin HAN, Runqiang YANG. Red light enhances folate accumulation in wheat seedlings[J]. Journal of Zhejiang University Science B, 2021, 22(11): 906-916.

@article{title="Red light enhances folate accumulation in wheat seedlings",
author="Jianwei CHANG, Chong XIE, Pei WANG, Zhenxin GU, Yongbin HAN, Runqiang YANG",
journal="Journal of Zhejiang University Science B",
volume="22",
number="11",
pages="906-916",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100266"
}

%0 Journal Article
%T Red light enhances folate accumulation in wheat seedlings
%A Jianwei CHANG
%A Chong XIE
%A Pei WANG
%A Zhenxin GU
%A Yongbin HAN
%A Runqiang YANG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 11
%P 906-916
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100266

TY - JOUR
T1 - Red light enhances folate accumulation in wheat seedlings
A1 - Jianwei CHANG
A1 - Chong XIE
A1 - Pei WANG
A1 - Zhenxin GU
A1 - Yongbin HAN
A1 - Runqiang YANG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 11
SP - 906
EP - 916
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100266


Abstract: 
Red, white, blue, green, and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings. The different lights, especially red light, significantly increased the total folate content. Total folate showed maximum accumulation under 30 μmol/(m2·s) of red light, with an increase of 24% compared with the control (darkness). 5-Methyl-tetrahydrofolate (5-CH3-THF) was the dominant folate component, and was significantly increased by red light irradiation. In addition, under red light, the folate content of leaves was higher and more sensitive to light than that of endosperm or roots. red light up-regulated the expression of guanosine triphosphate (GTP) cyclohydrolase 1 (GCH1) and aminodeoxychorismate synthase (ADCS), enhanced the activity of GCH1 and ADCS, and increased the content of precursors of folate synthesis, including pterin and p-aminobenzoic acid (pABA). Hence, the increased folate accumulation promoted by light could be attributed to the increased content of folate synthesis precursors, the activity of key enzymes, and related gene expression.

红光促进小麦芽苗中叶酸积累

目的:本研究旨在寻找刺激叶酸合成的最佳光谱和强度,并阐明红光调控小麦芽苗叶酸积累的机制。
创新点:在小麦芽苗生长过程中使用不同的光谱(白色、红色、蓝色、绿色和黄色)处理,发现了刺激叶酸合成的最佳光谱和强度,通过研究叶酸分布、叶酸生物合成中前体、酶和基因表达,进一步阐明了红光调控小麦芽苗叶酸积累的机制。
方法:小麦种子在25 °C下避光发芽2天后,第3天开始用30 µmol/(m2?s)的LED白光、红光(655 nm峰值)、蓝光(445 nm峰值)、绿光(520 nm峰值)和黄光(595 nm峰值)照射培养4天。光周期为16小时光照和8小时黑暗。在光照强度实验中,光照强度设置为0、15、30、45或60 µmol/(m2·s)。通过测定叶酸含量找出刺激叶酸合成的最佳光谱和强度。进一步研究最佳光照下小麦芽苗中叶酸分布、叶酸生物合成中前体、酶和基因表达。
结论:发芽和光处理增加了小麦芽苗叶酸的积累,30 µmol/(m2·s)红光照射下叶酸积累最多。红光促进小麦芽苗叶酸的积累主要表现在叶片中,尤其增加5-CH3-THF含量。此外,红光上调了叶酸合成相关基因表达,导致叶酸合成关键酶活性和前体含量的增加。

关键词:小麦;红光;光照强度;叶酸积累

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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