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On-line Access: 2021-11-16

Received: 2021-05-09

Revision Accepted: 2021-08-07

Crosschecked: 0000-00-00

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Bin LI

https://orcid.org/0000-0002-8348-7445

Junhong LÜ

https://orcid.org/0000-0003-2873-3137

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.11 P.966-970

http://doi.org/10.1631/jzus.B2100407


Response of Escherichia coli to hydrogen nanobubbles: an in vitro evaluation using synchrotron infrared spectroscopy


Author(s):  Jinfang LU, Jin ZHENG, Yadi WANG, Jie CHENG, Xueling LI, Jun HU, Bin LI, Junhong LÜ

Affiliation(s):  CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; more

Corresponding email(s):   lujunhong@zjlab.org.cn, libin@sinap.ac.cn

Key Words:  Molecular hydrogen, Nanobubbles, Bacteria, Infrared spectroscopy, Mechanism of action


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Jinfang LU, Jin ZHENG, Yadi WANG, Jie CHENG, Xueling LI, Jun HU, Bin LI, Junhong LÜ. Response of Escherichia coli to hydrogen nanobubbles: an in vitro evaluation using synchrotron infrared spectroscopy[J]. Journal of Zhejiang University Science B, 2021, 22(11): 966-970.

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Abstract: 
Hydrogen (H2)-rich water, an apparent source of molecular H2, is an emerging functional drink with many purported benefits for human health (Yang et al., 2020; Ostojic, 2021). The preventive and therapeutic effects of H2 on various pathological processes have been intensively investigated in numerous clinical trials; it is commonly believed that the beneficial effects are mainly attributed to its selective antioxidant and anti-inflammatory properties (Lee et al., 2015; Ohta, 2015; LeBaron et al., 2019; Qiu et al., 2020). In recent years, a handful of rodent studies revealed that exogenous H2 can affect the gut microbiota (Sha et al., 2018; Valdes et al., 2018). For example, H2 was reported to induce a higher abundance of butyrate-producing bacteria in a rat model of Parkinson's disease (Bordoni et al., 2019). Recent first-in-human trials have explored the effects of the long-term consumption of H2-rich water on antioxidant activity and the gut flora (Sha et al., 2018; Suzuki et al., 2018). Although these promising results suggest that the intestinal microbiota may be another plausible target for molecular H2, more studies are highly warranted to explain the mechanism(s) of H2 action on bacterial growth and functions.

大肠杆菌对氢纳米气泡的响应:使用同步红外光谱进行体外评估

目的:探究分子氢在细胞水平上的作用机制。
创新点:使用同步辐射红外光谱技术评估了氢纳米气泡在细胞水平上对大肠杆菌的影响,扩展了同步辐射红外光谱的应用范围。
方法:将大肠杆菌进行氢气纳米气泡处理后,使用同步辐射红外光谱测定其红外光谱,采用主成分分析等方法分析细胞成分的变化。
结论:氢纳米气泡处理后细胞的蛋白和脂肪酸成分发生较大改变,而核酸和多糖成分则没有明显变化。这些结果表明,氢气的作用靶点可能是细胞膜。此外,氢气分子的作用与传统的抗氧化剂(单宁)并不一致。

关键词:氢纳米气泡;同步辐射红外光谱;大肠杆菌

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Reference

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