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On-line Access: 2022-07-06

Received: 2021-11-15

Revision Accepted: 2022-03-21

Crosschecked: 2022-07-06

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Jiabian LIAN


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.7 P.587-596


ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma

Author(s):  Lu XIA, Hexin LIN, Yanming ZHOU, Jiabian LIAN

Affiliation(s):  Xiamen Cell Therapy Research Center, the First Affiliated Hospital of Xiamen University, Xiamen 361000, China; more

Corresponding email(s):   faster_lian@xmu.edu.cn

Key Words:  Zinc Finger Protein 750, Colon adenocarcinoma, Long non coding RNA, Chemotherapy

Lu XIA, Hexin LIN, Yanming ZHOU, Jiabian LIAN. ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma[J]. Journal of Zhejiang University Science B, 2022, 23(7): 587-596.

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author="Lu XIA, Hexin LIN, Yanming ZHOU, Jiabian LIAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma
%A Hexin LIN
%A Yanming ZHOU
%A Jiabian LIAN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 7
%P 587-596
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100939

T1 - ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma
A1 - Lu XIA
A1 - Hexin LIN
A1 - Yanming ZHOU
A1 - Jiabian LIAN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 7
SP - 587
EP - 596
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100939

The epidermal cell differentiation regulator zinc Finger Protein 750 (ZNF750) is a transcription factor containing the Cys2His2 (C2H2) domain, the zinc finger structure of which is located at the N-terminal 25‍‍–‍46 amino acids of ZNF750. It can promote the expression of differentiation-related factors while inhibiting the expression of progenitor cell-related genes. ZNF750 is directly regulated by p63 (encoded by the TP63 gene, belonging to the TP53 superfamily). The Krüppel-like factor 4 (KLF4), repressor element-1 (RE-1)‍-silencing transcription factor (REST) corepressor 1 (RCOR1), lysine demethylase 1A (KDM1A), and C-terminal-binding protein 1/2 (CTBP1/2) chromatin regulators cooperate with ZNF750 to repress epidermal progenitor genes and activate the expression of epidermal terminal differentiation genes (Sen et al., 2012; Boxer et al., 2014). Besides, ZNF750 and the regulatory network composed of bone morphogenetic protein (BMP) signaling pathway, long non-coding RNAs (lncRNAs) (anti-differentiation non-coding RNA (ANCR) and tissue differentiation-inducing non-protein coding RNA (TINCR)), musculoaponeurotic fibrosarcoma oncogene (MAF)/MAF family B (MAFB), grainy head-like 3 (GRHL3), and positive regulatory domain zinc finger protein 1 (PRDM1) jointly promote epidermal cell differentiation (Sen et al., 2012).


方法:在分子水平,利用蛋白质免疫印迹(western blotting)、染色质免疫沉淀-定量聚合酶链反应(ChIP-qPCR)、转录组测序(RNA-seq)、逆转录-定量聚合酶链反应(RT-qPCR)等技术手段对ZNF750和CYTOR在结肠腺癌细胞系中的表达进行分析;在细胞水平,通过使用克隆形成(colony formation)、小室迁移(transwell)等方法评估ZNF750对结肠腺癌增殖、侵袭、转移功能表型的影响;在动物水平,评估在免疫缺陷的裸鼠模型中,ZNF750对人结肠腺癌细胞系皮下移植瘤生长能力的影响;对TCGA和GEO数据库中结肠腺癌患者ZNF750-CYTOR的表达量、表达相关性进行生物信息学分析,并评估在对患者预后、肿瘤免疫浸润和常规化疗药物疗效预测中的价值。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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