Full Text:   <346>

Summary:  <41>

Suppl. Mater.: 

CLC number: 

On-line Access: 2022-07-06

Received: 2021-11-15

Revision Accepted: 2022-03-21

Crosschecked: 2022-07-06

Cited: 0

Clicked: 354

Citations:  Bibtex RefMan EndNote GB/T7714


Jiabian LIAN


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.7 P.587-596


ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma

Author(s):  Lu XIA, Hexin LIN, Yanming ZHOU, Jiabian LIAN

Affiliation(s):  Xiamen Cell Therapy Research Center, the First Affiliated Hospital of Xiamen University, Xiamen 361000, China; more

Corresponding email(s):   faster_lian@xmu.edu.cn

Key Words:  Zinc Finger Protein 750, Colon adenocarcinoma, Long non coding RNA, Chemotherapy

Lu XIA, Hexin LIN, Yanming ZHOU, Jiabian LIAN. ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma[J]. Journal of Zhejiang University Science B, 2022, 23(7): 587-596.

@article{title="ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma",
author="Lu XIA, Hexin LIN, Yanming ZHOU, Jiabian LIAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma
%A Hexin LIN
%A Yanming ZHOU
%A Jiabian LIAN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 7
%P 587-596
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100939

T1 - ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma
A1 - Lu XIA
A1 - Hexin LIN
A1 - Yanming ZHOU
A1 - Jiabian LIAN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 7
SP - 587
EP - 596
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100939

The epidermal cell differentiation regulator zinc Finger Protein 750 (ZNF750) is a transcription factor containing the Cys2His2 (C2H2) domain, the zinc finger structure of which is located at the N-terminal 25‍‍–‍46 amino acids of ZNF750. It can promote the expression of differentiation-related factors while inhibiting the expression of progenitor cell-related genes. ZNF750 is directly regulated by p63 (encoded by the TP63 gene, belonging to the TP53 superfamily). The Krüppel-like factor 4 (KLF4), repressor element-1 (RE-1)‍-silencing transcription factor (REST) corepressor 1 (RCOR1), lysine demethylase 1A (KDM1A), and C-terminal-binding protein 1/2 (CTBP1/2) chromatin regulators cooperate with ZNF750 to repress epidermal progenitor genes and activate the expression of epidermal terminal differentiation genes (Sen et al., 2012; Boxer et al., 2014). Besides, ZNF750 and the regulatory network composed of bone morphogenetic protein (BMP) signaling pathway, long non-coding RNAs (lncRNAs) (anti-differentiation non-coding RNA (ANCR) and tissue differentiation-inducing non-protein coding RNA (TINCR)), musculoaponeurotic fibrosarcoma oncogene (MAF)/MAF family B (MAFB), grainy head-like 3 (GRHL3), and positive regulatory domain zinc finger protein 1 (PRDM1) jointly promote epidermal cell differentiation (Sen et al., 2012).


方法:在分子水平,利用蛋白质免疫印迹(western blotting)、染色质免疫沉淀-定量聚合酶链反应(ChIP-qPCR)、转录组测序(RNA-seq)、逆转录-定量聚合酶链反应(RT-qPCR)等技术手段对ZNF750和CYTOR在结肠腺癌细胞系中的表达进行分析;在细胞水平,通过使用克隆形成(colony formation)、小室迁移(transwell)等方法评估ZNF750对结肠腺癌增殖、侵袭、转移功能表型的影响;在动物水平,评估在免疫缺陷的裸鼠模型中,ZNF750对人结肠腺癌细胞系皮下移植瘤生长能力的影响;对TCGA和GEO数据库中结肠腺癌患者ZNF750-CYTOR的表达量、表达相关性进行生物信息学分析,并评估在对患者预后、肿瘤免疫浸润和常规化疗药物疗效预测中的价值。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]BianZH, ZhangJW, LiM, et al., 2017. Long non-coding RNA LINC00152 promotes cell proliferation, metastasis, and confers 5-FU resistance in colorectal cancer by inhibiting miR-139-5p. Oncogenesis, 6(11):395.

[2]BoxerLD, BarajasB, TaoSY, et al., 2014. ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes. Genes Dev, 28(18):2013-2026.

[3]CaiJ, ZhangJ, WuP, et al., 2018. Blocking LINC00152 suppresses glioblastoma malignancy by impairing mesenchymal phenotype through the miR-612/AKT2/NF‍‍-‍‍κB pathway. J Neuro-Oncol, 140(2):225-236.

[4]CaiQ, WangZQ, WangSH, et al., 2016. Upregulation of long non-coding RNA LINC00152 by SP1 contributes to gallbladder cancer cell growth and tumor metastasis via PI3K/AKT pathway. Am J Transl Res, 8(10):‍4068-4081.

[5]CaiQ, WangZQ, WangSH, et al., 2017. Long non-coding RNA LINC00152 promotes gallbladder cancer metastasis and epithelial-mesenchymal transition by regulating HIF‍-‍‍1αvia miR-138. Open Biol, 7(1):160247.

[6]ChenPX, FangXL, XiaB, et al., 2018. Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR-125b with MCL-1 by regulating mitochondrial apoptosis pathways in ovarian cancer. Cancer Med, 7(9):4530-4541.

[7]ChenQN, ChenX, ChenZY, et al., 2017. Long intergenic non-coding RNA 00152 promotes lung adenocarcinoma proliferation via interacting with EZH2 and repressing IL24 expression. Mol Cancer, 16:17.

[8]ChenSA, ShenX, 2020. Long noncoding RNAs: functions and mechanisms in colon cancer. Mol Cancer, 19:167.

[9]DengX, ZhaoXF, LiangXQ, et al., 2017. Linc00152 promotes cancer progression in hepatitis B virus-associated hepatocellular carcinoma. Biomed Pharmacother, 90:100-108.

[10]HaoJJ, LinDC, DinhHQ, et al., 2016. Spatial intratumoral heterogeneity and temporal clonal evolution in esophageal squamous cell carcinoma. Nat Genet, 48(12):‍‍1500-1507.

[11]HazawaM, LinDC, HandralH, et al., 2017. ZNF750 is a lineage-specific tumour suppressor in squamous cell carcinoma. Oncogene, 36(16):2243-2254.

[12]JiJ, TangJW, DengL, et al., 2015. LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway. Oncotarget, 6(40):42813-42824.

[13]LuchiniC, BibeauF, LigtenbergMJL, et al., 2019. ESMO recommendations on microsatellite instability testing for immunotherapy in cancer, and its relationship with PD-1/PD-L1 expression and tumour mutational burden: a systematic review-based approach. Ann Oncol, 30(8):1232-1243.

[14]MaP, WangHT, SunJY, et al., 2018. LINC00152 promotes cell cycle progression in hepatocellular carcinoma via miR-193a/b-3p/CCND1 axis. Cell Cycle, 17(8):974-984.

[15]OuCL, SunZQ, HeXY, et al., 2020. Targeting YAP1/LINC00152/FSCN1 signaling axis prevents the progression of colorectal cancer. Adv Sci, 7(3):1901380.

[16]PardollDM, 2012. The blockade of immune checkpoints in cancer immunotherapy. Nat Rev Cancer, 12(4):252-264.

[17]QuJ, ZhangXQ, LvXY, 2020. Zinc finger protein 750 (ZNF750), negatively regulated by miR-17-5p, inhibits proliferation, motility and invasion of colonic cancer cells. J Gene Med, 22(8):e3195.

[18]SenGL, BoxerLD, WebsterDE, et al., 2012. ZNF750 is a p63 target gene that induces KLF4 to drive terminal epidermal differentiation. Dev Cell, 22(3):669-677.

[19]ShanYQ, YingRC, JiaZ, et al., 2017. LINC00052 promotes gastric cancer cell proliferation and metastasis via activating the Wnt/β‍-catenin signaling pathway. Oncol Res, 25(9):1589-1599.

[20]ShenX, ZhongJX, YuP, et al., 2019. YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein. Biochem Biophys Res Commun, 509(2):448-454.

[21]SunZH, GuoX, ZangMC, et al., 2019. Long non-coding RNA LINC00152 promotes cell growth and invasion of papillary thyroid carcinoma by regulating the miR-497/BDNF axis. J Cell Physiol, 234(2):1336-1345.

[22]WangHF, ChenWX, YangP, et al., 2019. Knockdown of linc00152 inhibits the progression of gastric cancer by regulating microRNA-193b-3p/ETS1 axis. Cancer Biol Ther, 20(4):461-473.

[23]WangX, YuHF, SunWJ, et al., 2018. The long non-coding RNA CYTOR drives colorectal cancer progression by interacting with NCL and Sam68. Mol Cancer, 17:110.

[24]WangYJ, LiuJZ, BaiHZ, et al., 2017. Long intergenic non-coding RNA 00152 promotes renal cell carcinoma progression by epigenetically suppressing P16 and negatively regulates miR-205. Am J Cancer Res, 7(2):312-322.

[25]WenRH, ChenC, ZhongXH, et al., 2021. PAX6 upstream antisense RNA (PAUPAR) inhibits colorectal cancer progression through modulation of the microRNA (miR)-17-5p/zinc finger protein 750 (ZNF750) axis. Bioengineered, 12(1):3886-3899.

[26]XiaT, LiaoQ, JiangXM, et al., 2014. Long noncoding RNA associated-competing endogenous RNAs in gastric cancer. Sci Rep, 4:6088.

[27]XuJS, GuoJJ, JiangYK, et al., 2019. Improved characterization of the relationship between long intergenic non-coding RNA Linc00152 and the occurrence and development of malignancies. Cancer Med, 8(10):4722-4731.

[28]XuSP, WanL, YinHZ, et al., 2017. Long noncoding RNA Linc00152 functions as a tumor propellant in pan-cancer. Cell Physiol Biochem, 44(6):2476-2490.

[29]YuY, YangJ, LiQP, et al., 2017. LINC00152: a pivotal oncogenic long non-coding RNA in human cancers. Cell Prolif, 50(4):e12349.

[30]YuMJ, XueYX, ZhengJ, et al., 2017. Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway. Mol Cancer, 16:110.

[31]YueB, CaiDL, LiuCC, et al., 2016. Linc00152 functions as a competing endogenous RNA to confer oxaliplatin resistance and holds prognostic values in colon cancer. Mol Ther, 24(12):2064-2077.

[32]YueB, LiuCC, SunHM, et al., 2018. A positive feed-forward loop between LncRNA-CYTOR and Wnt/β‍-catenin signaling promotes metastasis of colon cancer. Mol Ther, 26(5):1287-1298.

[33]ZhangPP, HeQP, LeiY, et al., 2018. m6A-mediated ZNF750 repression facilitates nasopharyngeal carcinoma progression. Cell Death Dis, 9(12):1169.

[34]ZhangYH, FuJ, ZhangZJ, et al., 2016. LncRNA-LINC00152 down-regulated by miR-376c-3p restricts viability and promotes apoptosis of colorectal cancer cells. Am J Transl Res, 8(12):5286-5297.

[35]ZhengLL, HuN, ZhouXZ, 2019. TCF3-activated LINC00152 exerts oncogenic role in osteosarcoma through regulating miR-1182/CDK14 axis. Pathol Res Pract, 215(2):373-380.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE