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On-line Access: 2023-04-10

Received: 2022-08-23

Revision Accepted: 2022-11-30

Crosschecked: 2023-04-14

Cited: 0

Clicked: 772

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Shuangquan WU

https://orcid.org/0000-0002-9352-8589

Qifa YE

https://orcid.org/0000-0002-2797-4804

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.4 P.345-351

http://doi.org/10.1631/jzus.B2200421


A novel ameliorated rat model of reversible obstructive jaundice


Author(s):  Yongkang ZOU, Pengpeng YUE, Hankun CAO, Liqin WU, Li XU, Zhongzhong LIU, Shuangquan WU, Qifa YE

Affiliation(s):  Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, National Quality Control Center for Donated Organ Procurement, Hubei Key Laboratory of Medical Technology on Transplantation, Hubei Clinical Research Center for Natural Polymer Biological Liver, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan 430071, China; more

Corresponding email(s):   yqf_china@163.com, shuangquanwu@whu.edu.cn

Key Words:  Animal model, Reversible obstructive jaundice, Bile duct ligation


Yongkang ZOU, Pengpeng YUE, Hankun CAO, Liqin WU, Li XU, Zhongzhong LIU, Shuangquan WU, Qifa YE. A novel ameliorated rat model of reversible obstructive jaundice[J]. Journal of Zhejiang University Science B, 2023, 24(4): 345-351.

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author="Yongkang ZOU, Pengpeng YUE, Hankun CAO, Liqin WU, Li XU, Zhongzhong LIU, Shuangquan WU, Qifa YE",
journal="Journal of Zhejiang University Science B",
volume="24",
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pages="345-351",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200421"
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Abstract: 
Obstructive jaundice is a common clinical symptom generally caused by bile duct stones, inflammatory hyperplasia, and tumors. It is characterized by hyperbilirubinemia and may trigger a variety of complications such as hypotension, kidney injury, endotoxemia, multiple organ dysfunction syndrome, and even death (Pavlidis and Pavlidis, 2018; Liu et al., 2021). Relieving bile duct obstruction and providing adequate drainage have been considered as the most effective therapies for obstructive jaundice. However, it has not yet been established whether it is beneficial to treat affected patients by pre-operative biliary drainage (Blacker et al., 2021). Moreover, the pathophysiological changes or mechanisms associated with the reversal of organ function following the relief of bile-duct obstruction are unclear (Huang et al., 2004). Therefore, it is necessary to establish an experimental model of reversible obstructive jaundice to simulate biliary drainage in clinical practice.

一种新型改良式可逆性梗阻性黄疸大鼠模型的建立

邹永康1,岳朋朋1,曹撼坤1,吴莉勤1,许力1,刘忠忠1,吴双全1,叶啟发1,2
1武汉大学中南医院,武汉大学肝胆疾病研究院,武汉大学移植医学中心,国家人体捐献器官获取质量控制中心,湖北省移植医疗技术重点实验室,湖北省天然高分子生物肝临床中心,湖北省天然高分子医用材料工程中心,中国武汉市,430071
2中南大学湘雅三院,国家卫生部移植医学工程技术研究中心,中国长沙市,410013
概要:现有的可逆性梗阻性黄疸动物模型存在许多缺陷,严重限制了其应用。一个简单、可靠的可逆性梗阻性黄疸动物模型值得进一步研究和探索。本研究利用改良式胆管结扎代替传统胆管结扎构建SD大鼠梗阻性黄疸模型,并基于改良式胆管结扎成功构建可逆性梗阻性黄疸模型。并通过肝功能、苏木精-伊红(HE)染色、Masson染色及天狼星红染色研究证实了胆道复通的有效性。进一步研究发现胆道复通7天后胆管细胞增殖和肝纤维化明显减轻,但其组织学病变尚未完全恢复正常;同时胆道复通7天后可显著降低CK19和衰老相关蛋白(p16及p21)的表达,但较正常组织仍高表达。因此,本研究成功建立了一种简单有效的可逆性梗阻性黄疸模型,其可有效地避免二次剖腹手术,显著降低死亡率,具有广泛的应用前景。

关键词:动物模型;可逆梗阻性黄疸;胆管结扎

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]AronsonDC, ChamuleauRAFM, FrederiksWM, et al., 1993. Reversibility of cholestatic changes following experimental common bile duct obstruction: fact or fantasy? J Hepatol, 18(1):85-95.

[2]BlackerS, LahiriRP, PhillipsM, et al., 2021. Which patients benefit from preoperative biliary drainage in resectable pancreatic cancer? Expert Rev Gastroenterol Hepatol, 15(8):855-863.

[3]DuYH, ChenH, XuanZF, et al., 2016. Bile deficiency induces changes in intestinal glucose absorption in mice. Surgery, 160(6):1496-1507.

[4]FrissenM, LiaoLJ, SchneiderKM, et al., 2021. Bidirectional role of NLRP3 during acute and chronic cholestatic liver injury. Hepatology, 73(5):1836-1854.

[5]HirataniS, MoriR, OtaY, et al., 2019. A simple and easily reproducible model of reversible obstructive jaundice in rats. In Vivo, 33(3):699-706.

[6]HuangLT, HsiehCS, ChouMH, et al., 2004. Obstructive jaundice in rats: cause of spatial memory deficits with recovery after biliary decompression. World J Surg, 28(3):283-287.

[7]HuangX, LiCH, ZhangAQ, et al., 2017. A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model. Ann Surg Treat Res, 92(6):389-395.

[8]JiCG, XieXL, YinJ, et al., 2017. Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice. Transl Res, 182:88-102.

[9]KahramansoyN, ErkolH, YilmazEE, et al., 2012. A new model of reversible obstructive jaundice using rapidly absorbable suture materials. Clin Invest Med, 35(6):E351-E357.

[10]LiW, ChungSCS, 2001. An improved rat model of obstructive jaundice and its reversal by internal and external drainage. J Surg Res, 101(1):4-15.

[11]LiuJY, QuJL, ChenHY, et al., 2021. The pathogenesis of renal injury in obstructive jaundice: a review of underlying mechanisms, inducible agents and therapeutic strategies. Pharmacol Res, 163:105311.

[12]LyuSC, WangJ, XuWL, et al., 2021. Therapeutic effect of combining anisodamine with neostigmine on local scar formation following Roux-en-Y choledochojejunostomy in a novel rat model. Front Pharmacol, 12:700050.

[13]OruçMT, ÖzmenMM, HanU, 2009. A new technique for inducing and releasing obstructive jaundice in rats. Eur Surg Res, 43(4):354-359.

[14]PavlidisET, PavlidisTE, 2018. Pathophysiological consequences of obstructive jaundice and perioperative management. Hepatobiliary Pancreat Dis Int, 17(1):17-21.

[15]WuLL, LiW, WangZK, et al., 2013. Bile acid-induced expression of farnesoid X receptor as the basis for superiority of internal biliary drainage in experimental biliary obstruction. Scand J Gastroenterol, 48(4):496-503.

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