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On-line Access: 2023-06-13

Received: 2022-09-08

Revision Accepted: 2023-01-27

Crosschecked: 2023-07-21

Cited: 0

Clicked: 640

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Chunmao HAN

https://orcid.org/0000-0002-2821-1323

Huawei SHAO

https://orcid.org/0000-0003-0310-9709

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.6 P.510-523

http://doi.org/10.1631/jzus.B2200447


Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization


Author(s):  Chuangang YOU, Zhikang ZHU, Shuangshuang WANG, Xingang WANG, Chunmao HAN, Huawei SHAO

Affiliation(s):  Department of Burns & Wound Care Center, the Second Affiliated Hospital, Zhejiang University School of Medicine,Hangzhou310009,China; more

Corresponding email(s):   2504131@zju.edu.cn, zrssk@zju.edu.cn

Key Words:  Nanosilver, Macrophages, Implants, Foreign body reaction, Wound repair


Chuangang YOU, Zhikang ZHU, Shuangshuang WANG, Xingang WANG, Chunmao HAN, Huawei SHAO. Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization[J]. Journal of Zhejiang University Science B, 2023, 24(6): 510-523.

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author="Chuangang YOU, Zhikang ZHU, Shuangshuang WANG, Xingang WANG, Chunmao HAN, Huawei SHAO",
journal="Journal of Zhejiang University Science B",
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pages="510-523",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200447"
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Abstract: 
foreign body reactions induced by macrophages often cause delay or failure of wound healing in the application of tissue engineering scaffolds. This study explores the application of nanosilver (NAg) to reduce foreign body reactions during scaffold transplantation. An NAg hybrid collagen-chitosan scaffold (NAg-CCS) was prepared using the freeze-drying method. The NAg-CCS was implanted on the back of rats to evaluate the effects on foreign body reactions. Skin tissue samples were collected for histological and immunological evaluation at variable intervals. Miniature pigs were used to assess the effects of NAg on skin wound healing. The wounds were photographed, and tissue samples were collected for molecular biological analysis at different time points post-transplantation. NAg-CCS has a porous structure and the results showed that it could release NAg constantly for two weeks. The NAg-CCS group rarely developed a foreign body reaction, while the blank-CCS group showed granulomas or necrosis in the subcutaneous grafting experiment. Both matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were reduced significantly in the NAg-CCS group. The NAg-CCS group had higher interleukin (IL)-10 and lower IL-6 than the blank CCS group. In the wound healing study, M1 macrophage activation and inflammatory-related proteins (inducible nitric oxide synthase (iNOS), IL-6, and interferon-‍γ (IFN-‍γ)) were inhibited by NAg. In contrast, M2 macrophage activation and proinflammatory proteins (arginase-1, major histocompatibility complex-II (MHC-II), and found in inflammatory zone-1 (FIZZ-1)) were promoted, and this was responsible for suppressing the foreign body responses and accelerating wound healing. In conclusion, dermal scaffolds containing NAg suppressed the foreign body reaction by regulating macrophages and the expression of inflammatory cytokines, thereby promoting wound healing.

纳米银通过调控巨噬细胞极化以减轻异物反应并促进创面修复

有传刚1,朱志康1,王双双1,2,王新刚1,韩春茂1,邵华伟1
1浙江大学医学院第二附属医院烧伤与创面修复科,中国杭州市,310009
2温岭市第一人民医院烧伤科,中国温岭市,317500
摘要:在组织工程支架的应用中,巨噬细胞引起的异物反应常导致伤口愈合延迟或失败。本研究探讨了纳米银(NAg)在支架移植过程中减少异物反应的作用。首先采用冷冻干燥法制备了包覆NAg的胶原-壳聚糖支架(NAg-CCS)。将Nag-CCS植入大鼠背部以评估对异物反应的影响,并在不同的时间间隔收集皮肤组织样本用于组织学和免疫学评估。小型猪创面愈合模型被用来评估NAg对皮肤伤口愈合的影响。在不同时间点对创面进行拍照,并收集组织样本用于移植后不同时间点的分子生物学分析。结果表明,NAg-CCS具有多孔结构,可以持续释放NAg超过2周。NAg-CCS组很少发生异物反应,而空白CCS组在皮下移植实验中出现明显的肉芽肿或坏死。NAg-CCS组的基质金属蛋白酶-1(MMP-1)和组织金属蛋白酶抑制剂-1(TIMP-1)均显着降低。Nag-CCS组比空白CCS组具有更高的白细胞介素(IL)-10和更低的IL-6。在创面愈合实验中,NAg显著抑制了M1巨噬细胞的活化和炎症相关蛋白(一氧化氮合成酶(iNOS)、IL-6和干扰素-γ(IFN-γ))的表达,而促进了M2巨噬细胞的活化和抗炎相关蛋白(精氨酸酶-1、主要组织相容性复合体-Ⅱ(MHC-Ⅱ)和抵抗素样分子-1(FIZZ-1))的表达,这些改变均有助于抑制创面的异物反应并加速创面的愈合。总之,含有NAg的真皮支架通过调节巨噬细胞极化和炎性细胞因子的表达来抑制异物反应,从而促进皮肤创面的修复。

关键词:纳米银;巨噬细胞;移植物;异物反应;创面修复

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]AlsalehNB,MinarchickVC,MendozaRP,et al.,2019.Silver nanoparticle immunomodulatory potential in absence of direct cytotoxicity in RAW 264.7 macrophages and MPRO 2.1 neutrophils.J Immunotoxicol,16(1):63-73.

[2]AndersonJM,JiangSR,2017.Implications of the acute and chronic inflammatory response and the foreign body reaction to the immune response of implanted biomaterials. In: Corradetti B (Ed.),The Immune Response to Implanted Materials and Devices: the Impact of the Immune System on the Success of an Implant.Springer,Cham, p.15-36.

[3]AndersonJM,RodriguezA,ChangDT,2008.Foreign body reaction to biomaterials.Semin Immunol,20(2):86-100.

[4]BenayahuD,PomeraniecL,ShemeshS,et al.,2020.Biocompatibility of a marine collagen-based scaffold in vitro and in vivo.Mar Drugs,18(8):420.

[5]BerganJJ,2005.Chronic venous insufficiency and the therapeutic effects of Daflon 500 mg.Angiology,56(6_suppl):S21-S24.

[6]ChiangYZ,PieroneG,Al-NiaimiF,2017.Dermal fillers: pathophysiology, prevention and treatment of complications.J Eur Acad Dermatol Venereol,31(3):405-413.

[7]ChuCY,LiuL,RungS,et al.,2020.Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment.J Biomed Mater Res Part A,108(1):127-135.

[8]ForbesJM,CooperME,2013.Mechanisms of diabetic complications.Physiol Rev,93(1):137-188.

[9]FurtadoM,ChenL,ChenZH,et al.,2022.Development of fish collagen in tissue regeneration and drug delivery.Eng Regener,3(3):217-231.

[10]GomesA,LeiteF,RibeiroL,2021.Adipocytes and macrophages secretomes coregulate catecholamine-synthesizing enzymes.Int J Med Sci,18(3):582-592.

[11]HeskethM,SahinKB,WestZE,et al.,2017.Macrophage phenotypes regulate scar formation and chronic wound healing.Int J Mol Sci,18(7):1545.

[12]HuangYJ,HungKC,HungHS,et al.,2018.Modulation of macrophage phenotype by biodegradable polyurethane nanoparticles: possible relation between macrophage polarization and immune response of nanoparticles.ACS Appl Mater Interfaces,10(23):19436-19448.

[13]JainN,VogelV,2018.Spatial confinement downsizes the inflammatory response of macrophages.Nat Mater,17(12):1134-1144.

[14]KimH,WangSY,KwakG,et al.,2019.Exosome-guided phenotypic switch of M1 to M2 macrophages for cutaneous wound healing.Adv Sci,6(20):1900513.

[15]LiCD,CuiWG,2021.3D bioprinting of cell-laden constructs for regenerative medicine.Eng Regener,2:195-205.

[16]LiuC,XuXY,CuiWG,et al.,2021.Metal-organic framework (MOF)‍-‍based biomaterials in bone tissue engineering. Eng Regener,2:105-108.

[17]LocatiM,CurtaleG,MantovaniA,2020.Diversity, mechanisms, and significance of macrophage plasticity.Annu Rev Pathol Mech Dis,15:123-147.

[18]MotzK,LinaI,MurphyMK,et al.,2021.M2 macrophages promote collagen expression and synthesis in laryngotracheal stenosis fibroblasts.Laryngoscope,131(2):E346-E353.

[19]OrecchioniM,GhoshehY,PramodAB,et al.,2019.Macrophage polarization: different gene signatures in M1(LPS+) vs. classically and M2(LPS-) vs. alternatively activated macrophages.Front Immunol,10:1084.

[20]O'SheaTM,WollenbergAL,KimJH,et al.,2020.Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions.Nat Commun,11:6203.

[21]PaulS,ChhatarS,MishraA,et al.,2019.Natural killer T cell activation increases iNOS+CD206- M1 macrophage and controls the growth of solid tumor.J ImmunoTher Cancer,7(1):208.

[22]SeoSY,LeeGH,LeeSG,et al.,2012.Alginate-based composite sponge containing silver nanoparticles synthesized in situ.Carbohydr Polym,90(1):109-115.

[23]SheikhZ,BrooksPJ,BarzilayO,et al.,2015.Macrophages, foreign body giant cells and their response to implantable biomaterials.Materials,8(9):5671-5701.

[24]ShiCY,WangCY,LiuH,et al.,2020.Selection of appropriate wound dressing for various wounds.Front Bioeng Biotechnol,8:182.

[25]ShiK,QiuX,ZhengW,et al.,2018.MiR-203 regulates keloid fibroblast proliferation, invasion, and extracellular matrix expression by targeting EGR1 and FGF2.Biomed Pharmacother,108:1282-1288.

[26]SnyderRJ,LantisJ,KirsnerRS,et al.,2016.Macrophages: a review of their role in wound healing and their therapeutic use.Wound Repair Regen,24(4):613-629.

[27]TanRZ,LiuJ,ZhangYY,et al.,2019.Curcumin relieved cisplatin-induced kidney inflammation through inhibiting Mincle-maintained M1 macrophage phenotype.Phytomedicine,52:284-294.

[28]VannellaKM,WynnTA,2017.Mechanisms of organ injury and repair by macrophages.Annu Rev Physiol,79:593-617.

[29]Villarreal-LealRA,HealeyGD,CorradettiB,2021.Biomimetic immunomodulation strategies for effective tissue repair and restoration.Adv Drug Deliv Rev,179:113913.

[30]WengTT,WuP,ZhangW,et al.,2020.Regeneration of skin appendages and nerves: current status and further challenges.J Transl Med,18:53.

[31]WicksK,TorbicaT,MaceKA,2014.Myeloid cell dysfunction and the pathogenesis of the diabetic chronic wound.Semin Immunol,26(4):341-353.

[32]WitherelCE,SaoK,BrissonBK,et al.,2021.Regulation of extracellular matrix assembly and structure by hybrid M1/M2 macrophages.Biomaterials,269:120667.

[33]WynnTA,VannellaKM,2016.Macrophages in tissue repair, regeneration, and fibrosis.Immunity,44(3):450-462.

[34]YangYX,ZhaoXD,YuJ,et al.,2021.Bioactive skin-mimicking hydrogel band-aids for diabetic wound healing and infectious skin incision treatment.Bioact Mater,6(11):‍3962-3975.

[35]YouCG,HanCM,WangXG,et al.,2012.The progress of silver nanoparticles in the antibacterial mechanism, clinical application and cytotoxicity.Mol Biol Rep,39(9):9193-9201.

[36]YouCG,LiQ,WangXG,et al.,2017.Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation.Sci Rep,7:10489.

[37]YunnaC,MengruH,LeiW,et al.,2020.Macrophage M1/M2 polarization.Eur J Pharmacol,877:173090.

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