CLC number:
On-line Access: 2023-04-10
Received: 2022-08-22
Revision Accepted: 2022-12-30
Crosschecked: 2023-04-14
Cited: 0
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Citations: Bibtex RefMan EndNote GB/T7714
Pan LIU, Mingfu LIU, Deshuang XI, Yiguang BAI, Ruixin MA, Yaomin MO, Gaofeng ZENG, Shaohui ZONG. Short-chain fatty acids ameliorate spinal cord injury recovery by regulating the balance of regulatory T cells and effector IL-17+ γδ T cells[J]. Journal of Zhejiang University Science B, 2023, 24(4): 312-325.
@article{title="Short-chain fatty acids ameliorate spinal cord injury recovery by regulating the balance of regulatory T cells and effector IL-17+ γδ T cells",
author="Pan LIU, Mingfu LIU, Deshuang XI, Yiguang BAI, Ruixin MA, Yaomin MO, Gaofeng ZENG, Shaohui ZONG",
journal="Journal of Zhejiang University Science B",
volume="24",
number="4",
pages="312-325",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200417"
}
%0 Journal Article
%T Short-chain fatty acids ameliorate spinal cord injury recovery by regulating the balance of regulatory T cells and effector IL-17+ γδ T cells
%A Pan LIU
%A Mingfu LIU
%A Deshuang XI
%A Yiguang BAI
%A Ruixin MA
%A Yaomin MO
%A Gaofeng ZENG
%A Shaohui ZONG
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 4
%P 312-325
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200417
TY - JOUR
T1 - Short-chain fatty acids ameliorate spinal cord injury recovery by regulating the balance of regulatory T cells and effector IL-17+ γδ T cells
A1 - Pan LIU
A1 - Mingfu LIU
A1 - Deshuang XI
A1 - Yiguang BAI
A1 - Ruixin MA
A1 - Yaomin MO
A1 - Gaofeng ZENG
A1 - Shaohui ZONG
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 4
SP - 312
EP - 325
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200417
Abstract: spinal cord injury (SCI) causes motor, sensory, and autonomic dysfunctions. The gut microbiome has an important role in SCI, while short-chain fatty acids (SCFAs) are one of the main bioactive mediators of microbiota. In the present study, we explored the effects of oral administration of exogenous SCFAs on the recovery of locomotor function and tissue repair in SCI. Allen’s method was utilized to establish an SCI model in Sprague-Dawley (SD) rats. The animals received water containing a mixture of 150 mmol/L SCFAs after SCI. After 21 d of treatment, the Basso, Beattie, and Bresnahan (BBB) score increased, the regularity index improved, and the base of support (BOS) value declined. Spinal cord tissue inflammatory infiltration was alleviated, the spinal cord necrosis cavity was reduced, and the numbers of motor neurons and Nissl bodies were elevated. Enzyme-linked immunosorbent assay (ELISA), real-time quantitative polymerase chain reaction (qPCR), and immunohistochemistry assay revealed that the expression of interleukin (IL)-10 increased and that of IL-17 decreased in the spinal cord. SCFAs promoted gut homeostasis, induced intestinal t cells to shift toward an anti-inflammatory phenotype, and promoted regulatory T (Treg) cells to secrete IL-10, affecting Treg cells and IL-17+ γ;δ; t cells in the spinal cord. Furthermore, we observed that Treg cells migrated from the gut to the spinal cord region after SCI. The above findings confirm that SCFAs can regulate Treg cells in the gut and affect the balance of Treg and IL-17+ γ;δ; t cells in the spinal cord, which inhibits the inflammatory response and promotes the motor function in SCI rats. Our findings suggest that there is a relationship among gut, spinal cord, and immune cells, and the “gut-spinal cord-immune” axis may be one of the mechanisms regulating neural repair after SCI.
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