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Weidong WANG


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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.9 P.779-795


Detection and quantitative analysis of tumor-associated tertiary lymphoid structures

Author(s):  Man YANG, Yurou CHE, Kezhen LI, Zengyi FANG, Simin LI, Mei WANG, Yiyao ZHANG, Zhu XU, Liping LUO, Chuan WU, Xin LAI, Weidong WANG

Affiliation(s):  Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China; more

Corresponding email(s):   wwdwyl@sina.com

Key Words:  Tertiary lymphoid structure, Tumor microenvironment, Chemokine signature, Spatial omics, Artificial intelligence, Radiomics

Man YANG, Yurou CHE, Kezhen LI, Zengyi FANG, Simin LI, Mei WANG, Yiyao ZHANG, Zhu XU, Liping LUO, Chuan WU, Xin LAI, Weidong WANG. Detection and quantitative analysis of tumor-associated tertiary lymphoid structures[J]. Journal of Zhejiang University Science B, 2023, 24(9): 779-795.

@article{title="Detection and quantitative analysis of tumor-associated tertiary lymphoid structures",
author="Man YANG, Yurou CHE, Kezhen LI, Zengyi FANG, Simin LI, Mei WANG, Yiyao ZHANG, Zhu XU, Liping LUO, Chuan WU, Xin LAI, Weidong WANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Detection and quantitative analysis of tumor-associated tertiary lymphoid structures
%A Yurou CHE
%A Kezhen LI
%A Zengyi FANG
%A Simin LI
%A Yiyao ZHANG
%A Zhu XU
%A Liping LUO
%A Chuan WU
%A Xin LAI
%A Weidong WANG
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 9
%P 779-795
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200605

T1 - Detection and quantitative analysis of tumor-associated tertiary lymphoid structures
A1 - Man YANG
A1 - Yurou CHE
A1 - Kezhen LI
A1 - Zengyi FANG
A1 - Simin LI
A1 - Mei WANG
A1 - Yiyao ZHANG
A1 - Zhu XU
A1 - Liping LUO
A1 - Chuan WU
A1 - Xin LAI
A1 - Weidong WANG
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 9
SP - 779
EP - 795
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200605

Tumor-associated tertiary lymphoid structures (TLSs) are ectopic lymphoid formations within tumor tissue, with mainly B and T cell populations forming the organic aggregates. The presence of TLSs in tumors has been strongly associated with patient responsiveness to immunotherapy regimens and improving tumor prognosis. Researchers have been motivated to actively explore TLSs due to their bright clinical application prospects. Various studies have attempted to decipher TLSs regarding their formation mechanism, structural composition, induction generation, predictive markers, and clinical utilization. Meanwhile, the scientific approaches to qualitative and quantitative descriptions are crucial for TLS studies. In terms of detection, hematoxylin and eosin (H&E), multiplex immunohistochemistry (mIHC), multiplex immunofluorescence (mIF), and 12-chemokine gene signature have been the top approved methods. However, no standard methods exist for the quantitative analysis of TLSs, such as absolute TLS count, analysis of TLS constituent cells, structural features, TLS spatial location, density, and maturity. This study reviews the latest research progress on TLS detection and quantification, proposes new directions for TLS assessment, and addresses issues for the quantitative application of TLSs in the clinic.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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