JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE A

Accepted manuscript available online (unedited version)

Random amplified polymorphic DNA reveals that TiO nanoparticles are genotoxic to

Author(s): Fabiola Moreno-Olivas^1;4, Vincent U. Gant Jr.², Kyle L. Johnson², Jose R. Peralta-Videa^1;3;4, Jorge L. Gardea-Torresdey^1;3;4
Affiliation(s): 1. Depertment of Chemistry, The University of Texas at El Paso, El Paso, TX 79968, USA;2. Department of Biological Sciences and Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA;3. Environmental Science and Engineering PhD Program, The University of Texas at El Paso, El Paso, TX 79968, USA;4. University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, El Paso, TX 79968, USA
Corresponding email(s): fmoreno2@miners.utep.edu
Key Words: Random amplified polymorphic DNA (RAPD), Titanium dioxide (TiO), Nanoparticles (NPs), Genomic DNA, Zucchini

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Fabiola Moreno-Olivas, Vincent U. Gant Jr. , Kyle L. Johnson, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey. Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo[J]. Journal of Zhejiang University Science A,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.A1400159

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author="Fabiola Moreno-Olivas, Vincent U. Gant Jr. , Kyle L. Johnson, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey",
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year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.A1400159"
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%T Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo
%A Fabiola Moreno-Olivas
%A Vincent U. Gant Jr.
%A Kyle L. Johnson
%A Jose R. Peralta-Videa
%A Jorge L. Gardea-Torresdey
%J Journal of Zhejiang University SCIENCE A
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doi="https://doi.org/10.1631/jzus.A1400159"

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A1 - Jose R. Peralta-Videa
A1 - Jorge L. Gardea-Torresdey
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doi="https://doi.org/10.1631/jzus.A1400159"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Titanium dioxide nanoparticles (TiO₂ NPs) are used in cosmetics, sunscreens, paints, and toothpaste, among other applications. These NPs are very stable and can be transported and dispersed in wastewater and biosolids. Animal species have shown negative reactions to TiO₂ NPs. However, little is known about their toxicity in plants, specifically the possibility of genotoxic effects. In this study, we used a random amplified polymorphic DNA (RAPD) technique to study the genotoxic effects of TiO₂ NPs on hydroponically cultivated zucchini (Cucurbita pepo) plants. Seeds were allowed to germinate for 7 d and plants were selected at random for individual and population studies. Four plants were selected for the individual study and 18 for the population study. RAPD profiles of TiO₂ NPs treated plants showed differences in band intensity, loss of bands, or appearance of new bands, compared to untreated plants. To the authors’ knowledge, this is the first report of the genotoxic potential of TiO₂ NPs in zucchini.

随机扩增多态性DNA技术研究发现二氧化钛纳米颗粒对西葫芦具有基因毒性

研究目的：二氧化钛（TiO2）纳米颗粒已经广泛应用于化妆品、防晒霜、涂料和牙膏等。这些纳米颗粒性质非常稳定，能在废水和生物固体中转移和分散。现有研究表明，TiO2纳米颗粒对动物正常生理活动具有毒性等负面作用。但是，它们对植物是否具有毒性特别是是否会产生植物基因毒性至今尚不清楚。因此，本文使用随机扩增多态性DNA技术研究TiO2纳米颗粒是否对西葫芦具有基因毒性，为TiO2纳米颗粒排放进入环境后的潜在植物毒性风险评价提供依据。
创新要点：首次发现了TiO2纳米颗粒对西葫芦具有基因毒性。
重要结论：采用随机扩增多态性DNA技术，发现TiO2纳米颗粒污染处理的西葫芦样品与未处理样品的基因组DNA图谱相比，不仅在谱带强度有明显差异，而且存在谱带消失和新谱带产生现象，表明TiO2纳米颗粒对西葫芦具有基因毒性。
随机扩增多态性DNA技术；TiO2；纳米颗粒；基因组DNA；西葫芦；毒性

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References

[1] Amaral,M.J, Carretero,M.A, Bicho,R.C, 2012, The use of a lacertid lizard as a model for reptile ecotoxicology studiespart 1 field demographics and morphology Chemosphere, 87(7):757-764.

[2] Cenkci,S, Yildiz,M, Ciğerci,�.�.H, 2009, Toxic chemicals-induced genotoxicity detected by random amplified polymorphic DNA (RAPD) in bean ( L.) seedlings Chemosphere, 76(7):900-906.

[3] Darlington,T.K, Neigh,A.M, Spencer,M.T, 2009, Nanoparticle characteristics affecting environmental fate and transport through soil Environmental Toxicology and Chemistry, 28(6):1191-1199.

[4] Gottschalk,F, Sonderer,T, Scholz,R.W, 2009, Modeled environmental concentrations of engineered nanomaterials (TiO, ZnO, Ag, CNT, fullerenes) for different regions Environonmental Science & Technology, 43(24):9216-9222.

[5] Hong,J, Peralta-Videa,J.R, Gardea-Torresdey,J.L, 2013, Nanomaterials in agricultural production: benefits and possible threats Sustainable Nanotechnology and the Environment: Advances and Achievements. ACS Symposium Series, 1124():73-90.

[6] Johnson,A.C, Bowes,M.J, Crossley,A, 2011, An assessment of the fate, behaviour and environmental risk associated with sunscreen TiO nanoparticles in UK field scenarios Science of the Total Environment, 409(13):2503-2510.

[7] Kang,S.J, Kim,B.M, Lee,Y.J, 2008, Titanium dioxide nanoparticles trigger p53-mediated damage response in peripheral blood lymphocytes Environmental and Molecular Mutagenesis, 49(5):399-405.

[8] Keller,A.A, Wang,H, Zhou,D, 2010, Stability and aggregation of metal oxide nanoparticles in natural aqueous media Environmental Science and Technology, 44(6):1962-1967.

[9] Keller,A.A, McFerran,S, Lazareva,A, 2013, Global life cycle releases of engineered nanomaterials Journal of Nanoparticle Research, 15(6):1692-.

[10] Kumari,M, Mukherjee,A, Chandrasekaran,N, 2009, Genotoxicity of silver nanoparticles in Science of the Total Environment, 407(19):5243-5246.

[11] Landa,P, Vankova,R, Andrlova,J, 2012, Nanoparticle-specific changes in gene expression after exposure to ZnO, TiO, and fullerene soot Journal of Hazardous Materials, 241-242():55-62.

[12] Liu,W, Li,P.J, Qi,X.M, 2005, DNA changes in barley () seedlings induced by cadmium pollution using RAPD analysis Chemosphere, 61(2):158-167.

[13] Liu,W, Yang,Y.S, Li,P.J, 2009, Risk assesment of cadmium-contaminated soil on plant DNA damage using RAPD and physiological indices Journal of Hazardous Materials, 161(2-3):878-883.

[14] Lpez-Moreno,M.L, de la Rosa,G, Hernndez-Viezcas,J, 2010, Evidence of the differential biotransformation and genotoxicity of ZnO and CeO nanoparticles on soybean () plants Environmental Science and Technology, 44(19):7315-7320.

[15] MacPherson,J.M, Gajadhar,A.A, 1992, Random amplified polymorphic DNA Parasitology Today, 8(7):235-.

[16] Peralta-Videa,J.R, Gardea-Torresdey,J.L, Gomez,E, 2002, Effect of mixed cadmium, copper, nickel and zinc at different pHs upon alfalfa growth and heavy metal uptake Environmental Pollution, 119(3):291-301.

[17] Petković,J, Zegura,B, Stevanović,M, 2011, DNA damage and alterations in expression of DNA damage responsive genes induced by TiO nanoparticles in human hepatoma HepG2 cells Nanotoxicology, 5(3):341-353.

[18] Robichaud,C.O, Uyar,A.E, Darby,M.R, 2009, Estimates of upper bounds and trends in nano-TiO production as basis for exposure assessment Environmental Science and Technology, 43(12):4227-4233.

[19] Roco,M.C, 2011, The long view of nanotechnology development: the national nanotechnology initiative at 10 years Journal of Nanoparticle Research, 13(2):427-445.

[20] Roco,M.C, Mirkin,C.A, Hersam,M.C, 2010, Nanotechnology research directions for societal needs in 2020: retrospective and outlook , ():-.

[21] Servin,A.D, Morales,M.I, Castillo-Michel,H, 2013, Synchrotron verification of TiO accumulation in cucumber fruit: a possible pathway of TiO nanoparticle transfer from soil into the food chain Environmental Science and Technology, 47(20):11592-11598.

[22] Williams,L, Adams,W, 2007, Nanotechnology Demystified. McGraw-Hill,New York, USA.

[23] Xue,C, Wu,J, Lan,F, 2010, Nano titanium dioxide induces the generation of ROS and potential damage in HaCaT cells under UVA irradiation Journal of Nanoscience and Nanotechnology, 10(12):8500-8507.

[24] Zheng,L, Su,M, Wu,X, 2008, Antioxidant stress is promoted by nano-anatase in spinach chloroplasts under UV-B radiation Biological Trace Element Research, 121(1):69-79.

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随机扩增多态性DNA技术研究发现二氧化钛纳米颗粒对西葫芦具有基因毒性

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References