JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

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Effect of vitamin B on cleft palate induced by 2,3,7,8-tetrachlorodibenzo--dioxin and dexamethasone in mice

Author(s): Shu-fan Zhao^1;2, Mao-zhou Chai^1;2, Min Wu³, Yong-hong He¹, Tian Meng^1;2, Bing Shi^1;2
Affiliation(s): 1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;2. Department of Cleft Lip and Palate Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;3. Department of Biochemistry & Molecular Biology, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58203, USA
Corresponding email(s): menglj123@126.com
Key Words: Cleft palate, Transforming growth factor-3 (TGF-3), Activin receptor-like kinase 5 (ALK5), Vitamin B , 2, 3, 7, 8-Tetrachlorodibenzo--dioxin, Dexamethasone

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Shu-fan Zhao, Mao-zhou Chai, Min Wu, Yong-hong He, Tian Meng, Bing Shi. Effect of vitamin B₁₂ on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1300083

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author="Shu-fan Zhao, Mao-zhou Chai, Min Wu, Yong-hong He, Tian Meng, Bing Shi",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B1300083"
}

%0 Journal Article
%T Effect of vitamin B₁₂ on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice
%A Shu-fan Zhao
%A Mao-zhou Chai
%A Min Wu
%A Yong-hong He
%A Tian Meng
%A Bing Shi
%J Journal of Zhejiang University SCIENCE B
%P 289-294
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B1300083"

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T1 - Effect of vitamin B₁₂ on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice
A1 - Shu-fan Zhao
A1 - Mao-zhou Chai
A1 - Min Wu
A1 - Yong-hong He
A1 - Tian Meng
A1 - Bing Shi
J0 - Journal of Zhejiang University Science B
SP - 289
EP - 294
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B1300083"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The purpose of this study was to investigate the effect of 2f4a8b>vitamin B₁₂ on palatal development by co-administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and dexamethasone (DEX). We examined the morphological and histological features of the palatal shelf and expression levels of key signaling molecules (transforming growth factor-β3 (TGF-β3) and TGF-β type I receptor (activin receptor-like kinase 5, ALK5)) during palatogenesis among a control group (Group A), TCDD+DEX exposed group (Group B), and TCDD+DEX+2f4a8b>vitamin B₁₂ exposed group (Group C). While we failed to find that 2f4a8b>vitamin B₁₂ decreased the incidence of 2f4a8b>cleft palate induced by TCDD+DEX treatment, the expression levels of key signaling molecules (TGF-β3 and ALK5) during palatogenesis were significantly modulated. In TCDD+DEX exposed and TCDD+DEX+2f4a8b>vitamin B₁₂ exposed groups, palatal shelves could not contact in the midline due to their small sizes. Our results suggest that 2f4a8b>vitamin B₁₂ may inhibit the expression of some 2f4a8b>cleft palate inducers such as TGF-β3 and ALK5 in DEX+TCDD exposed mice, which may be beneficial against palatogenesis to some degree, even though we were unable to observe a protective role of 2f4a8b>vitamin B₁₂ in morphological and histological alterations of palatal shelves induced by DEX and TCDD.

维生素B12对四氯二苯并对二恶英和地塞米松联合诱导腭裂小鼠拮抗性的初步研究

研究目的：通过四氯二苯并对二恶英（TCDD）和地塞米松（DEX）联合致畸构建更稳定的腭裂动物模型，初步研究维生素B₁₂拮抗剂对腭突发育的影响，检测信号分子变化，为后续研究提供参考。
创新要点：TCDD和DEX联合致畸可建立更稳定、更具研究价值的腭裂小鼠模型。从腭裂发生率、腭突发育形态大小、中嵴上皮细胞形态以及转化生长因子-β3（TGF-β3）、受体活化样激酶5（ALK5）分子表达等不同层次初步探讨了维生素B₁₂的拮抗作用。
研究方法：在小鼠腭发育关键时期，采用高剂量TCDD和DEX一次给药建立模型，并观察维生素B₁₂的拮抗作用（见图3）。于胚胎期17.5天（GD 17.5）体视显微镜下检测各组腭裂发生率（见表1和图2），并于GD 13.5、GD 14.5、GD 15.5 分别剪取胎鼠腭突提取RNA，采用实时荧光定量聚合酶链反应检测TGF-β3和ALK5基因表达（见图4、5）。
重要结论：TCDD和DEX联合作用可诱导C57BL/6J胎鼠形成稳定腭裂。维生素B₁₂对联合致畸诱导的TGF-β3及ALK5表达的改变及腭突中嵴上皮细胞形态的改变有一定程度的抑制作用，对腭裂发生率及腭突发育形态大小改变尚无明显抑制作用。

关键词组：腭裂；转化生长因子-β3；受体活化样激酶5；四氯二苯并对二恶英；地塞米松

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References

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维生素B12对四氯二苯并对二恶英和地塞米松联合诱导腭裂小鼠拮抗性的初步研究

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References