Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Author(s): Yixian Quah, Nor Ismaliza Mohd Ismail, Jillian Lean Sim Ooi, Yang Amri Affendi, Fazilah Abd Manan, Lai-Kuan Teh, Fai-Chu Wong, Tsun-Thai Chai
Affiliation(s): Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia; more
Corresponding email(s): chaitt@utar.edu.my
Key Words: Anticancer therapy; Bioactive peptide; Cytotoxicity; HeLa cells; Sarcophyton glaucum; Soft coral

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Yixian Quah, Nor Ismaliza Mohd Ismail, Jillian Lean Sim Ooi, Yang Amri Affendi, Fazilah Abd Manan, Lai-Kuan Teh, Fai-Chu Wong, Tsun-Thai Chai. Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1700586

@article{title="Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum",
author="Yixian Quah, Nor Ismaliza Mohd Ismail, Jillian Lean Sim Ooi, Yang Amri Affendi, Fazilah Abd Manan, Lai-Kuan Teh, Fai-Chu Wong, Tsun-Thai Chai",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B1700586"
}

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%T Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
%A Yixian Quah
%A Nor Ismaliza Mohd Ismail
%A Jillian Lean Sim Ooi
%A Yang Amri Affendi
%A Fazilah Abd Manan
%A Lai-Kuan Teh
%A Fai-Chu Wong
%A Tsun-Thai Chai
%J Journal of Zhejiang University SCIENCE B
%P 59-70
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%D in press
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doi="https://doi.org/10.1631/jzus.B1700586"

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T1 - Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum
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A1 - Nor Ismaliza Mohd Ismail
A1 - Jillian Lean Sim Ooi
A1 - Yang Amri Affendi
A1 - Fazilah Abd Manan
A1 - Lai-Kuan Teh
A1 - Fai-Chu Wong
A1 - Tsun-Thai Chai
J0 - Journal of Zhejiang University Science B
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doi="https://doi.org/10.1631/jzus.B1700586"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC₅₀) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC₅₀, AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.