JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Potential relationship between circulating gene expression of ACE2, TLR4, and Il-17 and disease severity and out-come of COVID-19 hospitalized patients

Author(s): Marwa A. DAHPY^1, ², Ragaa H. SALAMA¹, Abdel Raheim M.A. MEKI^1, ³, Ashraf Zein El-ABEDEEN⁴, Maiada K. HASHEM⁴, Ebtsam S. ABDULKAREEM⁵, Mohamed MOHANY⁶, Sinisa DJURASEVIC⁷, Amal N. IBRAHIM⁸, Nourhan M. HUSSEIN⁸ , Shima Gafar MANSOR⁹, Mohamed Ramadan IZZALDIN¹⁰, Marwa K. KHAIRALLAH¹¹, Suzan Eid Elshishtawy IBRAHIM¹², Alzahra ABDELBADEA¹³, Islam Khaled ali HARBY¹⁴, Fatma Y.A.ABBAS¹⁵, Rasha M. ALI¹⁵, Marwa A. SABET¹⁶, Salwa Seif ElDIN¹⁷, Abdelraouf M.S. ABDELRAOUF¹⁸, Amira A. KAMEL¹
Affiliation(s): ¹Department of Medical Biochemistry Faculty of Medicine-Assiut University, Assiut 71515, Egypt; more
Corresponding email(s): Marwadahpy@aun.edu.eg
Key Words: Acute respiratory distress syndrome (ARDS); COVID-19; Intensive Care Unit; Inter Leukin-17; Polymerase Chain Reaction; Toll Like Receptors-4

Share this article to： More <<< Previous Paper \|Next Paper >>>

Marwa A. DAHPY^1,², Ragaa H. SALAMA¹, Abdel Raheim M.A. MEKI^1,³, Ashraf Zein El-ABEDEEN⁴, Maiada K. HASHEM⁴, Ebtsam S. ABDULKAREEM⁵, Mohamed MOHANY⁶, Sinisa DJURASEVIC⁷, Amal N. IBRAHIM⁸, Nourhan M. HUSSEIN⁸ , Shima Gafar MANSOR⁹, Mohamed Ramadan IZZALDIN¹⁰, Marwa K. KHAIRALLAH¹¹, Suzan Eid Elshishtawy IBRAHIM¹², Alzahra ABDELBADEA¹³, Islam Khaled ali HARBY¹⁴, Fatma Y.A.ABBAS¹⁵, Rasha M. ALI¹⁵, Marwa A. SABET¹⁶, Salwa Seif ElDIN¹⁷, Abdelraouf M.S. ABDELRAOUF¹⁸, Amira A. KAMEL¹. Potential relationship between circulating gene expression of ACE2, TLR4, and Il-17 and disease severity and out-come of COVID-19 hospitalized patients[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400345

@article{title="Potential relationship between circulating gene expression of ACE2, TLR4, and Il-17 and disease severity and out-come of COVID-19 hospitalized patients",
author="Marwa A. DAHPY^1,², Ragaa H. SALAMA¹, Abdel Raheim M.A. MEKI^1,³, Ashraf Zein El-ABEDEEN⁴, Maiada K. HASHEM⁴, Ebtsam S. ABDULKAREEM⁵, Mohamed MOHANY⁶, Sinisa DJURASEVIC⁷, Amal N. IBRAHIM⁸, Nourhan M. HUSSEIN⁸ , Shima Gafar MANSOR⁹, Mohamed Ramadan IZZALDIN¹⁰, Marwa K. KHAIRALLAH¹¹, Suzan Eid Elshishtawy IBRAHIM¹², Alzahra ABDELBADEA¹³, Islam Khaled ali HARBY¹⁴, Fatma Y.A.ABBAS¹⁵, Rasha M. ALI¹⁵, Marwa A. SABET¹⁶, Salwa Seif ElDIN¹⁷, Abdelraouf M.S. ABDELRAOUF¹⁸, Amira A. KAMEL¹",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2400345"
}

%0 Journal Article
%T Potential relationship between circulating gene expression of ACE2, TLR4, and Il-17 and disease severity and out-come of COVID-19 hospitalized patients
%A Marwa A. DAHPY^1
%A²
%A Ragaa H. SALAMA¹
%A Abdel Raheim M.A. MEKI^1
%A³
%A Ashraf Zein El-ABEDEEN⁴
%A Maiada K. HASHEM⁴
%A Ebtsam S. ABDULKAREEM⁵
%A Mohamed MOHANY⁶
%A Sinisa DJURASEVIC⁷
%A Amal N. IBRAHIM⁸
%A Nourhan M. HUSSEIN⁸
%A Shima Gafar MANSOR⁹
%A Mohamed Ramadan IZZALDIN¹⁰
%A Marwa K. KHAIRALLAH¹¹
%A Suzan Eid Elshishtawy IBRAHIM¹²
%A Alzahra ABDELBADEA¹³
%A Islam Khaled ali HARBY¹⁴
%A Fatma Y.A.ABBAS¹⁵
%A Rasha M. ALI¹⁵
%A Marwa A. SABET¹⁶
%A Salwa Seif ElDIN¹⁷
%A Abdelraouf M.S. ABDELRAOUF¹⁸
%A Amira A. KAMEL¹
%J Journal of Zhejiang University SCIENCE B
%P
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2400345"

TY - JOUR
T1 - Potential relationship between circulating gene expression of ACE2, TLR4, and Il-17 and disease severity and out-come of COVID-19 hospitalized patients
A1 - Marwa A. DAHPY^{1
A1 -}²
A1 - Ragaa H. SALAMA¹
A1 - Abdel Raheim M.A. MEKI^{1
A1 -}³
A1 - Ashraf Zein El-ABEDEEN⁴
A1 - Maiada K. HASHEM⁴
A1 - Ebtsam S. ABDULKAREEM⁵
A1 - Mohamed MOHANY⁶
A1 - Sinisa DJURASEVIC⁷
A1 - Amal N. IBRAHIM⁸
A1 - Nourhan M. HUSSEIN⁸
A1 - Shima Gafar MANSOR⁹
A1 - Mohamed Ramadan IZZALDIN¹⁰
A1 - Marwa K. KHAIRALLAH¹¹
A1 - Suzan Eid Elshishtawy IBRAHIM¹²
A1 - Alzahra ABDELBADEA¹³
A1 - Islam Khaled ali HARBY¹⁴
A1 - Fatma Y.A.ABBAS¹⁵
A1 - Rasha M. ALI¹⁵
A1 - Marwa A. SABET¹⁶
A1 - Salwa Seif ElDIN¹⁷
A1 - Abdelraouf M.S. ABDELRAOUF¹⁸
A1 - Amira A. KAMEL¹
J0 - Journal of Zhejiang University Science B
SP -
EP -
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2400345"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Acute respiratory distress syndrome (ARDS) is a form of progressive hypoxemia that can be brought on by a variety of cardiorespiratory or systemic disorders, such as COVID-19. The binding of a SARS-Cov-2 virus spike protein to the cell membrane is mediated through its binding to the ACE2 receptors, resulting in viral entry, replication, and induction of a signaling cascade inducing pro-inflammatory responses that are linked to a higher mortality rate and the progression of ARDS, leading to multi-organ failure in these patients. We aimed to analyze the relationship between ACE2, TLR4, and Il-17 circulating gene-expression levels and the clinical severity of COVID-19 disease, as well as the associated pathogenic condition of hospitalized patients. Sixty COVID-19 patients (34 mild/moderate and 26 with severe ARDS manifestation) and 60 healthy controls were included. The patient group was also subdivided according to outcomes into 32 recoveries and 28 deaths. ACE2, TLR-4, and IL-17 levels were assessed by qPCR in addition to all routine baseline laboratory investigations, including CBC with differential analysis, CRP, ferritin, and D-dimer. ACE2, TLR-4, and IL-17 serum expression levels were significantly higher in the COVID-19 group and subgroups and correlated with different laboratory and clinical parameters. The serum expression levels of ACE2, TLR-4, and IL-17 were accurate in differentiating between the patient groups and controls with 86.7%, 91.7%, and 95% sensitivity and 96.7%, 98.3%, and 98.3% specificity, respectively, and correlate to more severe disease courses in COVID-19 patients. Higher levels are associated with overwhelmingly distressing outcomes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

Open peer comments: Debate/Discuss/Question/Opinion

<1>

- Go to

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference