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CLC number: O657.3

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.452-458

http://doi.org/10.1631/jzus.2006.B0452


Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin


Author(s):  FAN Ji-cai, CHEN Xiang, WANG Yun, FAN Cheng-ping, SHANG Zhi-cai

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   shangzc@mail.hz.zj.cn

Key Words:  Pefloxacin mesylate (PFLX), Bovine lactoferrin (BLf), Human serum albumin (HSA), Fluorescence spectra, Energy-transfer efficiency



Abstract: 
The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.

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