Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.2 P.194-200

http://doi.org/10.1631/jzus.B1200297

Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss

Author(s): S. M. Rahim^1,2,A. G. Mazlan³,K. D. Simon^2,3,J. P. Delaunoy⁴,P. Laurent⁵
Affiliation(s): 1. 1. Department of Biology, Faculty of Education, University of Tikrit, 34001 Tikrit, Salah Al Deen, Iraq more
Corresponding email(s): saleh_mr57@yahoo.com
Key Words: Pseudobranch, Carbonic anhydrase, Rainbow trout, Immunohistochemistry, Immunocytochemistry, Physiology

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S. M. Rahim, A. G. Mazlan, K. D. Simon, J. P. Delaunoy, P. Laurent. Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss[J]. Journal of Zhejiang University Science B, 2014, 15(2): 194-200.

@article{title="Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss",
author="S. M. Rahim, A. G. Mazlan, K. D. Simon, J. P. Delaunoy, P. Laurent",
journal="Journal of Zhejiang University Science B",
volume="15",
number="2",
pages="194-200",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200297"
}

%0 Journal Article
%T Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
%A S. M. Rahim
%A A. G. Mazlan
%A K. D. Simon
%A J. P. Delaunoy
%A P. Laurent
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 194-200
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200297

TY - JOUR
T1 - Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
A1 - S. M. Rahim
A1 - A. G. Mazlan
A1 - K. D. Simon
A1 - J. P. Delaunoy
A1 - P. Laurent
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 194
EP - 200
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1200297

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: pseudobranch function has long interested scientists, but its role has yet to be elucidated. Several studies have suggested that pseudobranchs serve respiratory, osmoregulatory, and sensory functions. This work investigated the immunolocalization of pseudobranch carbonic anhydrase (CA) in the teleost fish species rainbow trout (Oncorhynchus mykiss) to clarify its physiological function. CA was purified from rainbow trout gills O. mykiss and specific antibodies were raised. Immunoblotting between tissue homogenates of pseudobranch and gill CA antibodies showed specific immunostaining with only one band corresponding to CA in the pseudobranch homogenate. Results of immunohistochemical technique revealed that CA was distributed within pseudobranch cells and more precisely in the apical parts (anti-vascular) of cells. The basal (vascular) parts of cells, tubular system, blood capillaries, and pillar cells were not immunostained. immunocytochemistry confirmed these results and showed that some CA enzyme was cytoplasmic and the remainder was linked to membranous structures. The results also showed that the lacunar tissue layers did not display immunoperoxidase activity. Our results indicated that pseudobranch CA may have a function related to the extracellular medium wherein CA intervenes with the mechanism of stimulation of afferent nerve fibers.

虹鳟假鳃组织中的碳酸酐酶免疫细胞化学定位

研究目的：假腮的功能早已引起科学家兴趣，但还有待阐明。本文通过研究硬骨鱼类品种虹鳟鱼（Oncorhynchus mykiss）的假腮碳酸酐酶的免疫定位，来探讨假腮碳酸酐酶的生理功能。
研究方法：免疫组织化学染色技术。
重要结论：免疫组化结果显示碳酸酐酶分布在假腮细胞中，更精确地说是在其细胞顶端分布。细胞基底端、管状系统、毛细血管和柱细胞均无免疫染色。免疫细胞化学定位进一步验证了这些结果，并显示一部分是细胞质碳酸酐酶，其余的与细胞膜结构连接。此外，腔隙层未显示出免疫过氧化物酶的活性。本研究揭示了假腮碳酸酐酶的功能与细胞外介质有关，碳酸酐酶能干预传入神经纤维刺激机制。

关键词：碳酸酐酶；假鳃；虹鳟；免疫组化；免疫细胞化学；生理学