Full Text:   <3165>

Summary:  <1637>

CLC number: Q955

On-line Access: 2014-01-28

Received: 2012-11-02

Revision Accepted: 2013-03-05

Crosschecked: 2014-01-16

Cited: 5

Clicked: 9990

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.2 P.194-200

http://doi.org/10.1631/jzus.B1200297


Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss


Author(s):  S. M. Rahim1,2, A. G. Mazlan3, K. D. Simon2,3, J. P. Delaunoy4, P. Laurent5

Affiliation(s):  1. Department of Biology, Faculty of Education, University of Tikrit, 34001 Tikrit, Salah Al Deen, Iraq; more

Corresponding email(s):   saleh_mr57@yahoo.com

Key Words:  Pseudobranch, Carbonic anhydrase, Rainbow trout, Immunohistochemistry, Immunocytochemistry, Physiology


Share this article to: More <<< Previous Article|

S. M. Rahim, A. G. Mazlan, K. D. Simon, J. P. Delaunoy, P. Laurent. Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss[J]. Journal of Zhejiang University Science B, 2014, 15(2): 194-200.

@article{title="Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss",
author="S. M. Rahim, A. G. Mazlan, K. D. Simon, J. P. Delaunoy, P. Laurent",
journal="Journal of Zhejiang University Science B",
volume="15",
number="2",
pages="194-200",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200297"
}

%0 Journal Article
%T Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
%A S. M. Rahim
%A A. G. Mazlan
%A K. D. Simon
%A J. P. Delaunoy
%A P. Laurent
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 194-200
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200297

TY - JOUR
T1 - Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
A1 - S. M. Rahim
A1 - A. G. Mazlan
A1 - K. D. Simon
A1 - J. P. Delaunoy
A1 - P. Laurent
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 194
EP - 200
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200297


Abstract: 
pseudobranch function has long interested scientists, but its role has yet to be elucidated. Several studies have suggested that pseudobranchs serve respiratory, osmoregulatory, and sensory functions. This work investigated the immunolocalization of pseudobranch carbonic anhydrase (CA) in the teleost fish species rainbow trout (Oncorhynchus mykiss) to clarify its physiological function. CA was purified from rainbow trout gills O. mykiss and specific antibodies were raised. Immunoblotting between tissue homogenates of pseudobranch and gill CA antibodies showed specific immunostaining with only one band corresponding to CA in the pseudobranch homogenate. Results of immunohistochemical technique revealed that CA was distributed within pseudobranch cells and more precisely in the apical parts (anti-vascular) of cells. The basal (vascular) parts of cells, tubular system, blood capillaries, and pillar cells were not immunostained. immunocytochemistry confirmed these results and showed that some CA enzyme was cytoplasmic and the remainder was linked to membranous structures. The results also showed that the lacunar tissue layers did not display immunoperoxidase activity. Our results indicated that pseudobranch CA may have a function related to the extracellular medium wherein CA intervenes with the mechanism of stimulation of afferent nerve fibers.

虹鳟假鳃组织中的碳酸酐酶免疫细胞化学定位

研究目的:假腮的功能早已引起科学家兴趣,但还有待阐明。本文通过研究硬骨鱼类品种虹鳟鱼(Oncorhynchus mykiss)的假腮碳酸酐酶的免疫定位,来探讨假腮碳酸酐酶的生理功能。
研究方法:免疫组织化学染色技术。
重要结论:免疫组化结果显示碳酸酐酶分布在假腮细胞中,更精确地说是在其细胞顶端分布。细胞基底端、管状系统、毛细血管和柱细胞均无免疫染色。免疫细胞化学定位进一步验证了这些结果,并显示一部分是细胞质碳酸酐酶,其余的与细胞膜结构连接。此外,腔隙层未显示出免疫过氧化物酶的活性。本研究揭示了假腮碳酸酐酶的功能与细胞外介质有关,碳酸酐酶能干预传入神经纤维刺激机制。

关键词:碳酸酐酶;假鳃;虹鳟;免疫组化;免疫细胞化学;生理学

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

References

[1] Berenbrink, M., 2007. Historical reconstructions of evolving physiological complexity: O2 secretion in the eye and swim bladder of fishes. J Exp Biol, 210(9):1641-1652. 


[2] Bridges, C.R., Berenbrink, M., Mller, R., 1998. Physiology and biochemistry of the pseudobranch: an unanswered question?. Comp Biochem Physiol Part A Mol & Int Physiol, 119(1):67-77. 


[3] Broussonet, P.M.A., 1785. Mémoire pour servir à l′histoire de la respiration des Poissons. Mem Acad R Sci Paris, (in French),:174-196. 

[4] Cammer, W., Tansey, F.A., 1987. Immunocytochemical localization of carbonic anhydrase in myelinated fiber in peripheral nerves of rat and mouse. J Histochem Cytochem, 35(8):865-870. 


[5] Churg, A., 1973. Carbonic anhydrase histochemistry: evidence for non-enzymatic reaction and artifact production. Histochimie, 36(4):293-302. 


[6] Delaunoy, J.P., 1983.  Etude du I' Anhydrase Carbonique (EC4.2.II) Dans le System Nerveux Central du Rat. (in French), PhD Thesis, University of Louis-Pasteur,Strasbourg, France :

[7] Dimberg, K., 1995. Investigation of pseudobranch organ in rainbow trout (Oncorhyncus mykiss): endogenous substrates and activities of carbonic anhydrase, lactate dehydrogenase and 3-hydroxy-acyl CoA dehydrogenase. Fish Physiol Biochem, 14(4):323-327. 


[8] Ekinci, D., Ceyhun, S.B., Sentrk, M., 2011. Characterization and anions inhibition studies of an α-carbonic anhydrase from the teleost fish Dicentrarchus labraxBioorg Med Chem, 19(2):744-748. 


[9] Esbaugh, A.J., Tufts, B.L., 2006. The structure and function of carbonic anhydrase isoenzymes in the respiratory system of vertebrates. Respir Physiol Neurobiol, 154(1):185-198. 


[10] Esbaugh, A.J., Perry, S.F., Bayaa, M., 2005. Cytoplasmic carbonic anhydrase isozymes in rainbow trout Oncorhynchus mykiss: comparative physiology and molecular evolution. J Exp Biol, 208(10):1951-1961. 


[11] Filippi, D., Sciaky, M., Limozin, N., 1978. Anhydrase carbonique de system nerveux central du rat. Isolement et proprieties. Biochimie, (in French),60:99-102. 


[12] Georgalis, T., Perry, S.F., Gilmour, K.M., 2006. The role of branchial carbonic anhydrase in acid-base regulation in rainbow trout (Oncorhynchus mykiss). J Exp Biol, 209(3):518-530. 


[13] Georgalis, T., Gilmour, K.M., Yorston, J., 2006. Roles of cytosolic and membrane-bound carbonic anhydrase in renal control of acid-base balance in rainbow trout (Oncorhynchus mykiss). Am J Physiol Renal Physiol, 291(2):F407-F421. 


[14] Gilmour, K.M., Perry, S.F., 2009. Carbonic anhydrase and acid-base regulation in fish. J Exp Biol, 212(11):1647-1661. 


[15] Graham, R., Karnovsky, M.J., 1966. The early stage of adsorption of injected horse radish peroxidase in the proximal tubules of mouse kidney: ultrstructurale cytochemistry by a new technique. J Histochem Cytochem, 14(4):291-302. 


[16] Hansson, H.P.J., 1967. Histochemical demonstration of carbonic anhydrase activity. Histochimie, 11(2):112-128. 


[17] Hassan, I., Shajee, B., Waheed, A., 2012. Structure, function and application of carbonic anhydrase isozymes. Bioorg Med Chem, 21(6):1570-1582. 


[18] Hyrtl, J., 1838. Beobachtungen aus dem gebiete der vergleichenden gefasslehere. II. Uber den bau der kiemen der fische. Med Jahrb, (in German),15:232-248. 

[19] Kumpulainen, T., 1981. Human carbonic anhydrase isozyme C. Histochemistry, 72(3):425-431. 


[20] Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259):680-685. 


[21] Laurent, P., 1967. La pseudobranchie des téléostéens: preuves electrophsiologiques de ses fonctions chémoréceptrice et baroréceptrice. CR Hebd Sances Acad Sci, (in French),264:1879-1882. 

[22] Laurent, P., 1974. Pseudobranchial receptors in teleosts. Electroreceptors and Other Specialized Receptors in Lower Vertrebrates, Springer Verlag,:279-296. 


[23] Laurent, P., Rouzeau, J., 1972. Afferent neural activity from pseudobranch of teleost. Effect of P O2 , pH, osmotic pressure and Na+ ions. Respir Physiol, 14(3):307-331. 


[24] Laurent, P., Dunel-Erb, S., 1984. The pseudobranch: morphology and function.  Fish Physiology. Academic Press,New York Vol. X:285-325. 

[25] Laurent, P., Dunel, S., Barets, A., 1969. Localisation histochimique de l’anhydrase carbonique au niveau de chémorécepteurs artériels de mammifères, des batraciens et des poisons. Histochimie, (in French),17(2):99-107. 


[26] Lin, T.Y., Liao, B.K., Horng, J.L., 2008. Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells. Am J Physiol Cell Physiol, 294(5):C1250-C1260. 


[27] Maetz, J., 1956. Le rôle biologique de l'anhydrase carbonique chez quelques téléostéens. Bull Biol France-Belgique Suppl, (in French),XL:1-129. 

[28] Mlich, A., Waser, W., Heisler, N., 2009. The teleost pseudobranch: a role for preconditioning of ocular blood supply?. Fish Physiol Biochem, 35(2):273-286. 


[29] Muther, T.F., 1977. On the lack of specificity of the cobalt bicarbonate method for carbonic anhydrase. J Histochem Cytochem, 25(9):1043-1050. 


[30] Quinn, M.C.J., Veillette, P.A., Young, G., 2003. Pseudobranch and gill Na+, K+-ATPase activity in juvenile Chinook salmon, Oncorhynchus tshaytscha: developmental changes and effect of growth hormone, cortisol and seawater transfer. Comp Biochem Physiol Part A Mol Int Physiol, 135(2):249-262. 


[31] Rahim, S.M., 1988.  Contribution l' tude de l' Anhydrase Carbonique des Poissons: Mise en Evidence de Deux Isoenzymes rythrocytaire et Branchiale. (in French), PhD Thesis, University Louis Pasteur,Strasbourg, France :

[32] Rahim, S.M., Delaunoy, J.P., Laurent, P., 1988. Identification and immunocytochemical localization of two carbonic anhydrase isoenzymes in teleostean fish erythrocytes and gill epithelia. Histochemistry, 89(5):451-459. 


[33] Rummer, J.L., Brauner, C.J., 2011. Plasma-accessible carbonic anhydrase at the tissue of a teleost fish may greatly enhance oxygen delivery: in vitro evidence in rainbow trout, Oncorhynchus mykissJ Exp Biol, 214(14):2319-2328. 


[34] Sethi, K.K., Verma, S.M., Kumar, P.M., 2011. Carbonic anhydrase I and II inhibition with natural products: Leucas cephalotesPharmacogn Commun, 1(2):41-46. 


[35] Supuran, C.T., 2008. Carbonic anhydrase—an overview. Curr Pharm Des, 14(7):603-614. 


[36] Towbin, H., Staekelin, T., Gordon, T., 1979. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. PNAS, 76(9):4350-4354. 


[37] Waser, W., Heisler, N., 2005. Oxygen delivery to the fish eye: root effect as crucial factor for elevated retinal P O2 J Exp Biol, 208(21):4035-4047. 



Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE