JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.6 P.548-555

Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats*

Author(s): Sitthichai Iamsaard^1,2, Jaturon Burawat^1,2, Pipatpong Kanla¹, Supatcharee Arun^1,2, Wannisa Sukhorum^1,2, Bungorn Sripanidkulchai², Nongnut Uabundit¹, Jintanaporn Wattathorn¹, Wiphawi Hipkaeo¹, Duriya Fongmoon³, Hisatake Kondo¹
Affiliation(s): 1. Department of Anatomy and Integrative Complementary Alternative Medicine Research and Development Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; more
Corresponding email(s): iamsaard_sitt@yahoo.com
Key Words: Clitoria ternatea (CT) flower extracts, Antioxidant activity, Ketoconazole (KET), Testicular damage, Testicular tyrosine phosphorylation

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Sitthichai Iamsaard, Jaturon Burawat, Pipatpong Kanla, Supatcharee Arun, Wannisa Sukhorum, Bungorn Sripanidkulchai, Nongnut Uabundit, Jintanaporn Wattathorn, Wiphawi Hipkaeo, Duriya Fongmoon, Hisatake Kondo. Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats[J]. Journal of Zhejiang University Science B, 2014, 15(6): 548-555.

@article{title="Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats",
author="Sitthichai Iamsaard, Jaturon Burawat, Pipatpong Kanla, Supatcharee Arun, Wannisa Sukhorum, Bungorn Sripanidkulchai, Nongnut Uabundit, Jintanaporn Wattathorn, Wiphawi Hipkaeo, Duriya Fongmoon, Hisatake Kondo",
journal="Journal of Zhejiang University Science B",
volume="15",
number="6",
pages="548-555",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300299"
}

%0 Journal Article
%T Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats
%A Sitthichai Iamsaard
%A Jaturon Burawat
%A Pipatpong Kanla
%A Supatcharee Arun
%A Wannisa Sukhorum
%A Bungorn Sripanidkulchai
%A Nongnut Uabundit
%A Jintanaporn Wattathorn
%A Wiphawi Hipkaeo
%A Duriya Fongmoon
%A Hisatake Kondo
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 6
%P 548-555
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300299

TY - JOUR
T1 - Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats
A1 - Sitthichai Iamsaard
A1 - Jaturon Burawat
A1 - Pipatpong Kanla
A1 - Supatcharee Arun
A1 - Wannisa Sukhorum
A1 - Bungorn Sripanidkulchai
A1 - Nongnut Uabundit
A1 - Jintanaporn Wattathorn
A1 - Wiphawi Hipkaeo
A1 - Duriya Fongmoon
A1 - Hisatake Kondo
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 6
SP - 548
EP - 555
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300299

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Background: ketoconazole (KET), an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria ternatea (CT) are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported. Objective: To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET. Methods: The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Male rats were treated with CT flower extracts (10, 50, or 100 mg/kg BW) or distilled water via a gastric tube for 28 d (preventive period: Days 1–21) and induced by KET (100 mg/kg BW) via intraperitoneal injection for 7 d (induction period: Days 22–28). After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting. Results: The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT+KET groups, CT flower extracts (50 and 100 mg/kg BW) alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100+KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups. Conclusions: C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats.

蝶豆花提取物的抗氧化活性及其对酮康唑诱导大鼠睾丸损伤的保护作用

研究目的：研究蝶豆花提取物的抗氧化活性及其对酮康唑（KET）诱导雄性大鼠睾丸损伤的保护作用。
创新要点：对蝶豆花提取物保护酮康唑引起的雄性动物睾丸损伤进行研究，为减轻抗真菌药物酮康唑的生殖毒性提供理论依据和解决途径。
研究方法：采用2,2-二苯基-1-苦肼基自由基（DPPH）分析法和亚铁还原能力实验法（FRAP）来测定蝶豆花提取物的抗氧化活性。在为期28天的动物试验中，前21天为预防期，第21至28天为KET诱导期。整个试验期中，对雄性大鼠进行蝶豆花提取物（0、50、100 mg/kg BW）灌胃饲养；在诱导期，同时腹腔注射KET (100 mg/kg BW)。试验结束后，测定睾丸、附睾和输精管以及精囊的重量、血清睾酮水平、精子浓度、组织结构、睾丸的直径和睾丸酪氨酸磷酸化水平。
重要结论：蝶豆花提取物具有清除DPPH自由基能力和较高的还原能力，其在100 mg/kg BW下对雄性大鼠生殖系统无毒。50、100 mg/kg BW蝶豆花提取物能改善KET引起的生殖器官重量下降、血清睾酮水平和精子浓度，降低KET引起的睾丸损伤。与其他组相比，100 mg/kg BW蝶豆花提取物能显著提高睾丸中50-kDa的酪氨酸磷酸化蛋白的表达。由此可见，具有抗氧化活性的蝶豆花提取物不会损伤雄性生殖系统，而且具有保护KET诱导大鼠睾丸损伤的作用。

关键词：蝶豆花提取物；抗氧化活性；酮康唑（KET）；睾丸损伤；酪氨酸磷酸化

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蝶豆花提取物的抗氧化活性及其对酮康唑诱导大鼠睾丸损伤的保护作用

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References