Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.6 P.436-444

http://doi.org/10.1631/jzus.B1700277


Protection of plasma transfusion against lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure through inhibiting apoptosis of hepatic cells in mice


Author(s):  Bing-yu Chen, Lu-xi Jiang, Ke Hao, Lu Wang, Ying Wang, Yi-wei Xie, Jian Shen, Meng-hua Zhu, Xiang-ming Tong, Kai-qiang Li, Zhen Wang

Affiliation(s):  1. Research Center of Blood Transfusion Medicine, Ministry of Education Key Laboratory of Laboratory Medicine, Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou 310014, China more

Corresponding email(s):   lkq252526@163.com, zhenwangzjpph@163.com

Key Words:  Fulminant hepatic failure, Plasma, Inflammation, Apoptosis


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Bing-yu Chen, Lu-xi Jiang, Ke Hao, Lu Wang, Ying Wang, Yi-wei Xie, Jian Shen, Meng-hua Zhu, Xiang-ming Tong, Kai-qiang Li, Zhen Wang. Protection of plasma transfusion against lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure through inhibiting apoptosis of hepatic cells in mice[J]. Journal of Zhejiang University Science B, 2018, 19(6): 436-444.

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author="Bing-yu Chen, Lu-xi Jiang, Ke Hao, Lu Wang, Ying Wang, Yi-wei Xie, Jian Shen, Meng-hua Zhu, Xiang-ming Tong, Kai-qiang Li, Zhen Wang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="6",
pages="436-444",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700277"
}

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%T Protection of plasma transfusion against lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure through inhibiting apoptosis of hepatic cells in mice
%A Bing-yu Chen
%A Lu-xi Jiang
%A Ke Hao
%A Lu Wang
%A Ying Wang
%A Yi-wei Xie
%A Jian Shen
%A Meng-hua Zhu
%A Xiang-ming Tong
%A Kai-qiang Li
%A Zhen Wang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 6
%P 436-444
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700277

TY - JOUR
T1 - Protection of plasma transfusion against lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure through inhibiting apoptosis of hepatic cells in mice
A1 - Bing-yu Chen
A1 - Lu-xi Jiang
A1 - Ke Hao
A1 - Lu Wang
A1 - Ying Wang
A1 - Yi-wei Xie
A1 - Jian Shen
A1 - Meng-hua Zhu
A1 - Xiang-ming Tong
A1 - Kai-qiang Li
A1 - Zhen Wang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 6
SP - 436
EP - 444
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700277


Abstract: 
fulminant hepatic failure is a severe clinical condition associated with extremely poor outcomes and high mortality. A number of studies have demonstrated the ability of plasma transfusion to successfully treat fulminant hepatic failure, but the underlying mechanisms are not well understood. The aim of the present study is to define the mechanisms of plasma transfusion treatment in lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced mice. LPS/D-GalN treatment in mice causes significant hepatic failure, including increasing serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, histopathological changes in centrilobular necrosis and inflammatory cells, and the up-regulation of inflammation (tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)). When LPS/D-GaIN-induced mice were treated with plasma, these changes were halted. Results showed that plasma transfusion significantly reduced mortality, and decreased the levels of AST, ALT, and inflammation factors such as TNF-α and IL-6. The expression levels of cleaved Caspase-3, BAX, and p53 were down-regulated and Bcl-2 was up-regulated, suggesting that plasma can reduce LPS/D-GalN-induced apoptosis. The protective mechanism of plasma against LPS/D-GalN-induced fulminant hepatic failure is related to the inhibition of the inflammatory response and the reduction in apoptosis through the down-regulation of the p53-induced apoptotic pathway.

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Full Text:   <4652>

Summary:  <3109>

CLC number: Q291

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2018-05-14

Cited: 0

Clicked: 8044

Citations:  Bibtex RefMan EndNote GB/T7714

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