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Received: 2004-02-05

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.2 P.125-131


Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+

Author(s):  YU Hai-ning, SHEN Sheng-rong, XIONG Yao-kang

Affiliation(s):  Department of Tea Science, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Copper, Epigallocatechin-3-gallate, LNCaP cells, Tea

YU Hai-ning, SHEN Sheng-rong, XIONG Yao-kang. Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+[J]. Journal of Zhejiang University Science B, 2005, 6(2): 125-131.

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author="YU Hai-ning, SHEN Sheng-rong, XIONG Yao-kang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+
%A YU Hai-ning
%A SHEN Sheng-rong
%A XIONG Yao-kang
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 2
%P 125-131
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0125

T1 - Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+
A1 - YU Hai-ning
A1 - SHEN Sheng-rong
A1 - XIONG Yao-kang
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 2
SP - 125
EP - 131
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0125

epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions, such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu2+. EGCG was incubated with prostate cancer cells, LNCaP, pretreated with or without Cu2+. EGCG in F-12 medium was quantified using HPLC and the viability of cells was assessed by gel electrophoresis, flow cytometry, and electron microscope. The results of HPLC showed that EGCG degraded completely within 12 h in F-12 medium with or without Cu2+. Gel electrophoresis and flow cytometry did not detect apoptosis of LNCaP cells when they were incubated with EGCG. Electron microscopy examination revealed that EGCG-Cu2+ complex led to damage of cytoplasm membrane in LNCaP cells. It was speculated that not EGCG, but its oxide and complex with Cu2+, are the bioactive components responsible for its cytotoxicity to LNCaP prostate cancer cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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