Similar articles

Abstract: cucurbitaceae is an important family of flowering plants containing multiple species of important food plants, such as melons, cucumbers, squashes, and pumpkins. However, a highly efficient genetic transformation system has not been established for most of these species (Nanasato and Tabei, 2020). watermelon (Citrullus lanatus), an economically important and globally cultivated fruit crop, is a model species for fruit quality research due to its rich diversity of fruit size, shape, flavor, aroma, texture, peel and flesh color, and nutritional composition (Guo et al., 2019). Through pan-genome sequencing, many candidate loci associated with fruit quality traits have been identified (Guo et al., 2019). However, few of these loci have been validated. The major barrier is the low transformation efficiency of the species, with only few successful cases of genetic transformation reported so far (Tian et al., 2017; Feng et al., 2021; Wang JF et al., 2021; Wang YP et al., 2021). For example, Tian et al. (2017) obtained only 16 transgenic lines from about 960 cotyledon fragments, yielding a transformation efficiency of 1.67%. Therefore, efficient genetic transformation could not only facilitate the functional genomic studies in watermelon as well as other horticultural species, but also speed up the transgenic and genome-editing breeding.

生长调节基因介导的西瓜高效遗传转化与CRISPR/Cas9基因编辑体系

[1]DebernardiJM, TricoliDM, ErcoliMF, et al., 2020. A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nat Biotechnol, 38(11):1274-1279.

[2]FengQ, XiaoL, HeYZ, et al., 2021. Highly efficient, genotype-independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric CLGRF4-GIF1 gene. J Integr Plant Biol, 63(12):2038-2042.

[3]GuoSG, ZhaoSJ, SunHH, et al., 2019. Resequencing of 414 cultivated and wild watermelon accessions identifies selection for fruit quality traits. Nat Genet, 51(11):1616-1623.

[4]KongJX, Martin-OrtigosaS, FinerJ, et al., 2020. Overexpression of the transcription factor GROWTH-REGULATING FACTOR5 improves transformation of dicot and monocot species. Front Plant Sci, 11:572319.

[5]LoweK, WuE, WangN, et al., 2016. Morphogenic regulators Baby boom and Wuschel improve monocot transformation. Plant Cell, 28(9):1998-2015.

[6]Méndez-Hernández1 HA, Ledezma-Rodríguez M, Avilez-Montalvo RN,et al., 2019. Signaling overview of plant somatic embryogenesis. Front Plant Sci, 10:77.

[7]NanasatoY, TabeiY, 2020. A method of transformation and current progress in transgenic research on cucumbers and Cucurbita species. Plant Biotechnol (Tokyo), 37(2):141-146.

[8]ParkSM, LeeJS, JegalS, et al., 2005. Transgenic watermelon rootstock resistant to CGMMV (cucumber green mottle mosaic virus) infection. Plant Cell Rep, 24(6):350-356.

[9]TanYY, DuH, WuX, et al., 2020. Gene editing: an instrument for practical application of gene biology to plant breeding. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 21(6):460-473. http://doi.org/10.1631/jzus.B1900633

[10]TianSW, JiangLJ, GaoQ, et al., 2017. Efficient CRISPR/Cas9-based gene knockout in watermelon. Plant Cell Rep, 36(3):399-406.

[11]WangJF, WangYP, ZhangJ, et al., 2021. The NAC transcription factor CLNAC68 positively regulates sugar content and seed development in watermelon by repressing CLINV and CLGH3.6. Hortic Res, 8:214.

[12]WangYP, WangJF, GuoSG, et al., 2021. CRISPR/Cas9-mediated mutagenesis of CLBG1 decreased seed size and promoted seed germination in watermelon. Hortic Res, 8:70.

[13]XingHL, DongL, WangZP, et al., 2014. A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol, 14:327.

[14]ZhangXY, XuGC, ChengCH, et al., 2021. Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated targeted mutagenesis in hemp (Cannabis sativa L.). Plant Biotechnol J, 19(10):1979-1987.