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Anyong XIE


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.1 P.73-86


Target binding and residence: a new determinant of DNA double-strand break repair pathway choice in CRISPR/Cas9 genome editing

Author(s):  Yili FENG, Sicheng LIU, Ruodan CHEN, Anyong XIE

Affiliation(s):  Innovation Center for Minimally Invasive Technique and Device, Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310019, China; more

Corresponding email(s):   eric_feng@zju.edu.cn, anyongxie@zju.edu.cn

Key Words:  CRISPR/Cas9 genome editing, Double-strand break (DSB) repair pathway choice, Target binding affinity, Target residence

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Yili FENG, Sicheng LIU, Ruodan CHEN, Anyong XIE. Target binding and residence: a new determinant of DNA double-strand break repair pathway choice in CRISPR/Cas9 genome editing[J]. Journal of Zhejiang University Science B, 2021, 22(1): 73-86.

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author="Yili FENG, Sicheng LIU, Ruodan CHEN, Anyong XIE",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Target binding and residence: a new determinant of DNA double-strand break repair pathway choice in CRISPR/Cas9 genome editing
%A Yili FENG
%A Sicheng LIU
%A Ruodan CHEN
%A Anyong XIE
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 1
%P 73-86
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000282

T1 - Target binding and residence: a new determinant of DNA double-strand break repair pathway choice in CRISPR/Cas9 genome editing
A1 - Yili FENG
A1 - Sicheng LIU
A1 - Ruodan CHEN
A1 - Anyong XIE
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 1
SP - 73
EP - 86
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000282

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is widely used for targeted genomic and epigenomic modifications and imaging in cells and organisms, and holds tremendous promise in clinical applications. The efficiency and accuracy of the technology are partly determined by the target binding affinity and residence time of Cas9-single-guide RNA (sgRNA) at a given site. However, little attention has been paid to the effect of target binding affinity and residence duration on the repair of Cas9-induced DNA double-strand breaks (DSBs). We propose that the choice of DSB repair pathway may be altered by variation in the binding affinity and residence duration of Cas9-sgRNA at the cleaved target, contributing to significantly heterogeneous mutations in CRISPR/Cas9 genome editing. Here, we discuss the effect of Cas9-sgRNA target binding and residence on the choice of DSB repair pathway in CRISPR/Cas9 genome editing, and the opportunity this presents to optimize Cas9-based technology.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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