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Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing
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Qiongxi Pan, Chenyuan Gao, Yingying Wang, Yili Wang, Cong Mao, Quan Wang, Sophia N. Economidou, Dennis Douroumis, Feng Wen, Lay Poh Tan & Huaqiong Li . Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing[J]. Journal of Zhejiang University Science D, 2020, 3(4): 396-409.
@article{title="Investigation of bone reconstruction using an attenuated
immunogenicity xenogenic composite scafold fabricated by 3D
printing",
author="Qiongxi Pan, Chenyuan Gao, Yingying Wang, Yili Wang, Cong Mao, Quan Wang, Sophia N. Economidou, Dennis Douroumis, Feng Wen, Lay Poh Tan & Huaqiong Li ",
journal="Journal of Zhejiang University Science D",
volume="3",
number="4",
pages="396-409",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00086-4"
}
%0 Journal Article
%T Investigation of bone reconstruction using an attenuated
immunogenicity xenogenic composite scafold fabricated by 3D
printing
%A Qiongxi Pan
%A Chenyuan Gao
%A Yingying Wang
%A Yili Wang
%A Cong Mao
%A Quan Wang
%A Sophia N. Economidou
%A Dennis Douroumis
%A Feng Wen
%A Lay Poh Tan & Huaqiong Li
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 4
%P 396-409
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00086-4
TY - JOUR
T1 - Investigation of bone reconstruction using an attenuated
immunogenicity xenogenic composite scafold fabricated by 3D
printing
A1 - Qiongxi Pan
A1 - Chenyuan Gao
A1 - Yingying Wang
A1 - Yili Wang
A1 - Cong Mao
A1 - Quan Wang
A1 - Sophia N. Economidou
A1 - Dennis Douroumis
A1 - Feng Wen
A1 - Lay Poh Tan & Huaqiong Li
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 4
SP - 396
EP - 409
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00086-4
Abstract: Bone is known to have a natural function to heal itself. However, if the bone damage is beyond a critical degree, intervention
such as bone grafting may be imperative. In this work, the fabrication of a novel bone scafold composed of natural bone
components and polycaprolactone (PCL) using 3D printing is put forward. α1, 3-galactosyltransferase defcient pigs were
used as the donor source of a xenograft. decellularized porcine bone (DCB) with attenuated immunogenicity was used as
the natural component of the scafold with the aim to promote bone regeneration. The 3D printed DCB-PCL scafolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive
strength. The biological properties of the DCB-PCL scafolds were evaluated by studying cell adhesion, viability, alkaline
phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem cells. The in vitro
results demonstrated that the DCB-PCL scafolds exhibit an enhanced performance in promoting bone diferentiation, which
is correlated to the DCB content. Furthermore, critical-sized cranial rat defects were used to assess the efect of DCB-PCL
scafolds on bone regeneration in vivo. The results confrm that in comparison with PCL scafolds, the DCB-PCL scafolds
can signifcantly improve new bone formation in cranial defects. Thus, the proposed 3D printed DCB-PCL scafolds emerge
as a promising regeneration alternative in the clinical treatment of large bone defects.
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