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Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation
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Feng Zhang, Kaiyun Qu, Xiaopei Li, Chaoming Liu, Lazarus S. Ortiz, Kaihong Wu, Xiaowei Wang, Ningping Huang. Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation[J]. Journal of Zhejiang University Science D, 2021, 4(1): 100-110.
@article{title="Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation",
author="Feng Zhang, Kaiyun Qu, Xiaopei Li, Chaoming Liu, Lazarus S. Ortiz, Kaihong Wu, Xiaowei Wang, Ningping Huang",
journal="Journal of Zhejiang University Science D",
volume="4",
number="1",
pages="100-110",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00100-9"
}
%0 Journal Article
%T Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation
%A Feng Zhang
%A Kaiyun Qu
%A Xiaopei Li
%A Chaoming Liu
%A Lazarus S. Ortiz
%A Kaihong Wu
%A Xiaowei Wang
%A Ningping Huang
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 1
%P 100-110
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00100-9
TY - JOUR
T1 - Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation
A1 - Feng Zhang
A1 - Kaiyun Qu
A1 - Xiaopei Li
A1 - Chaoming Liu
A1 - Lazarus S. Ortiz
A1 - Kaihong Wu
A1 - Xiaowei Wang
A1 - Ningping Huang
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 1
SP - 100
EP - 110
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00100-9
Abstract: Cardiovascular diseases are the leading cause of morbidity and mortality throughout the world underlining the importance of efficient treatments including disease modeling and drug discovery by cardiac tissue engineering. However, the predictive power of these applications is currently limited by the immature state of the cardiomyocytes. Here, we developed gelatin hydrogels chemically crosslinked by genipin, a biocompatible crosslinker, as cell culture scaffolds. Neonatal rat cardiomyocytes appear synchronous beating within 2 days after seeding on hydrogels. Furthermore, we applied the electrical stimulation as a conditioning treatment to promote the maturation of cardiomyocytes cultured on the hydrogels. Our results show that electrical stimulation improves the organization of sarcomeres, establishment of gap junctions, calcium-handling capacity and propagation of pacing signals, thereby, increase the beating velocity of cardiomyocytes and responsiveness to external pacing. The above system can be applied in promoting physiological function maturation of engineered cardiac tissues, exhibiting promising applications in cardiac tissue engineering and drug screening.
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