CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 0
Clicked: 137
Bilgesu Kaya, Ozlem Yesil-Celiktas. Ionic liquid-based transparent membrane-coupled human lung epithelium-on-a-chip demonstrating PM0.5 pollution effect under breathing mechanostress[J]. Journal of Zhejiang University Science D, 2024, 7(5): 624-636.
@article{title="Ionic liquid-based transparent membrane-coupled human lung
epithelium-on-a-chip demonstrating PM0.5 pollution effect
under breathing mechanostress",
author="Bilgesu Kaya, Ozlem Yesil-Celiktas",
journal="Journal of Zhejiang University Science D",
volume="7",
number="5",
pages="624-636",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00289-z"
}
%0 Journal Article
%T Ionic liquid-based transparent membrane-coupled human lung
epithelium-on-a-chip demonstrating PM0.5 pollution effect
under breathing mechanostress
%A Bilgesu Kaya
%A Ozlem Yesil-Celiktas
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 5
%P 624-636
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00289-z
TY - JOUR
T1 - Ionic liquid-based transparent membrane-coupled human lung
epithelium-on-a-chip demonstrating PM0.5 pollution effect
under breathing mechanostress
A1 - Bilgesu Kaya
A1 - Ozlem Yesil-Celiktas
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 5
SP - 624
EP - 636
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00289-z
Abstract: The plausibility of human exposure to particulate matter (PM) has witnessed an increase within the last several years. PM of
different sizes has been discovered in the atmosphere given the role of dust transport in weather and climate composition. As
a regulator, the lung epithelium orchestrates the innate response to local damage. Herein, we developed a lung epithelium-ona-chip platform consisting of easily moldable polydimethylsiloxane layers along with a thin, flexible, and transparent ionic
liquid-based poly(hydroxyethyl) methacrylate gel membrane. The epithelium was formed through the culture of human lung
epithelial cells (Calu-3) on this membrane. The mechanical stress at the airliquid interface during inhalation/exhalation was
recapitulated using an Arduino-based servo motor system, which applied a uniaxial tensile strength from the two sides of the
chip with 10% strain and a frequency of 0.2 Hz. Subsequently, the administration of silica nanoparticles (PM0.5) with an
average size of 463 nm to the on-chip platform under static, dynamic, and dynamic + mechanical stress (DMS) conditions
demonstrated the effect of environmental pollutants on lung epithelium. The viability and release of lactate dehydrogenase
were determined along with proinflammatory response through the quantification of tumor necrosis factor-?, which indicated
alterations in the epithelium.
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