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CLC number: Q291; R34

On-line Access: 2012-05-04

Received: 2011-12-24

Revision Accepted: 2012-03-27

Crosschecked: 2012-04-18

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.5 P.356-363

http://doi.org/10.1631/jzus.B1100386


Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration


Author(s):  Xiao-ying Wang, Zhong-hua Chen, Ru-yi Zhang, Sen-quan Liu, Zhu Mei, Ying-ying Yu, Xiong Zhang, Qiang Xia, Yue-min Ding

Affiliation(s):  Department of Gastroenterology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   dingyuemin@zucc.edu.cn

Key Words:  Cell migration, Bone morphogenetic protein-2 (BMP-2), COS-7, Cofilin


Xiao-ying Wang, Zhong-hua Chen, Ru-yi Zhang, Sen-quan Liu, Zhu Mei, Ying-ying Yu, Xiong Zhang, Qiang Xia, Yue-min Ding. Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration[J]. Journal of Zhejiang University Science B, 2012, 13(5): 356-363.

@article{title="Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration",
author="Xiao-ying Wang, Zhong-hua Chen, Ru-yi Zhang, Sen-quan Liu, Zhu Mei, Ying-ying Yu, Xiong Zhang, Qiang Xia, Yue-min Ding",
journal="Journal of Zhejiang University Science B",
volume="13",
number="5",
pages="356-363",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100386"
}

%0 Journal Article
%T Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration
%A Xiao-ying Wang
%A Zhong-hua Chen
%A Ru-yi Zhang
%A Sen-quan Liu
%A Zhu Mei
%A Ying-ying Yu
%A Xiong Zhang
%A Qiang Xia
%A Yue-min Ding
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 5
%P 356-363
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100386

TY - JOUR
T1 - Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration
A1 - Xiao-ying Wang
A1 - Zhong-hua Chen
A1 - Ru-yi Zhang
A1 - Sen-quan Liu
A1 - Zhu Mei
A1 - Ying-ying Yu
A1 - Xiong Zhang
A1 - Qiang Xia
A1 - Yue-min Ding
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 5
SP - 356
EP - 363
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100386


Abstract: 
Objective: Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms. Methods: Human BMP-2 genetic fragment was amplified and introduced into the pEGFP-C1 vector. After being confirmed by XhoI and BamHI digestion analyses and DNA sequencing, the recombinant pEGFP-C1-BMP-2 plasmid was transfected into COS-7 cells. The influence of BMP-2 on cell migration and cofilin activity was detected by cell scratch assay and Western blotting. Results: The recombinant pEGFP-C1-BMP-2 was effectively expressed in COS-7 cells. An increased phosphorylation of both LIMK1 and cofilin and an enhancement of cell migration were observed in cells with overexpression of BMP-2. Conclusions: A recombinant pEGFP-C1-BMP-2 vector was successfully constructed and overexpression of BMP-2 regulated the activities of the downstream molecules of the Rho GTPase signaling pathway, which might contribute to the enhancement of cell migration.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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