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Bio-Design and Manufacturing  2022 Vol.5 No.1 P.220-232

http://doi.org/10.1007/s42242-021-00144-5


Novel pneumatically assisted atomization device for living cell delivery: application of sprayed mesenchymal stem cells for skin regeneration


Author(s):  Lixing Zhang, Xintao Yan, Li An, Meijia Wang, Xi Xu, Zhonglin Ma, Mengting Nie, Fangzhou Du, Jingzhong Zhang & Shuang Yu

Affiliation(s):  Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China; more

Corresponding email(s):   zhangjz@sibet.ac.cn, yush@sibet.ac.cn

Key Words:  Cell delivery, Skin wound, Wound healing, Spray application, Cell-spray autografting, Stem cell therapy


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Lixing Zhang, Xintao Yan, Li An, Meijia Wang, Xi Xu, Zhonglin Ma, Mengting Nie, Fangzhou Du, Jingzhong Zhang & Shuang Yu. Novel pneumatically assisted atomization device for living cell delivery: application of sprayed mesenchymal stem cells for skin regeneration[J]. Journal of Zhejiang University Science D, 2022, 5(1): 220-232.

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author="Lixing Zhang, Xintao Yan, Li An, Meijia Wang, Xi Xu, Zhonglin Ma, Mengting Nie, Fangzhou Du, Jingzhong Zhang & Shuang Yu",
journal="Journal of Zhejiang University Science D",
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year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00144-5"
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Abstract: 
Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired. Recently, topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration. However, stem cells must be administrated uniformly to the wound area, otherwise treatment will be ineffective, which has been a limitation of current administration methods. Specifically, the delivery pressure and nozzle features of most clinical cell spray devices are unknown, which may significantly affect the viability of sprayed cells and their capacity for proliferation. Herein, we developed a novel pneumatically assisted atomization device (PAAD) in which cell suspensions were uniformly atomized at a low delivery pressure. We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types, while ensuring uniform dispersal across the wound site. Moreover, we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model. Overall, the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells, strongly suggesting its potential for application in clinical cell therapy, especially for large, irregular, and severe skin wounds.

中科院苏州生物医学工程技术研究所张丽星等 | 新型气动辅助雾化装置的制备及其递送细胞在皮肤组织再生中的应用

本研究论文聚焦新型气动辅助雾化装置的制备及其递送细胞在皮肤组织再生中的应用。大面积皮肤损伤已成为严重医疗问题,甚至威胁患者生命。近年来,局部干细胞治疗被认为是皮肤创面修复以及皮肤再生的有效治疗方法。但是,干细胞必须均匀地注入到创面,否则将影响治疗效果,分布不均是目前细胞给药方法的局限性。临床上大多数细胞喷洒装置的输送压力不可精确调控,喷嘴特性对细胞压力的影响尚不清楚,这可能会显著影响喷洒细胞的生存和增殖能力。本文开发了一种新型的气动辅助雾化装置(PAAD),该装置能在较低传递压力下,实现细胞悬浮液的均匀雾化。我们通过优化系统的液体压力和气体压力,最大限度地维持了多种细胞的生存活性和功能特点。此外,我们发现将喷洒的脐带间充质干细胞应用于伤口部位,可显著加速伤口愈合和促进皮肤附属器官再生。综上所述,新型气动辅助雾化装置能均匀地输送活细胞,并维持喷洒后细胞的活力和分化能力,这表明该装置具有极大的潜力应用于临床创面治疗,特别是针对大的,不规则的,以及严重的皮肤伤口。

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