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Bio-Design and Manufacturing  2023 Vol.6 No.3 P.284-297

http://doi.org/10.1007/s42242-022-00227-x


Gelatin-based hydrogel functionalized with taurine moieties for in vivo skin tissue regeneration


Author(s):  Farnaz Rahimi, Nima Ahmadkhani, Aida Goodarzi, Fariba Noori, Sajad Hassanzadeh, Sepideh Saghati, Mehdi Khanmohammadi & Arash Goodarzi

Affiliation(s):  Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Science, Fasa, Iran; more

Corresponding email(s):   Mehdi.khanmohammadi84@gmail.com, khanmohammadi.mehdi@iums.ac.ir, dvm.goodarzi86@yahoo.com

Key Words:  Taurine conjugation, Covalent crosslinking, Immobilization of growth factors, Gelatin-based hydrogel, Endothelial cells


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Farnaz Rahimi, Nima Ahmadkhani, Aida Goodarzi, Fariba Noori, Sajad Hassanzadeh, Sepideh Saghati, Mehdi Khanmohammadi & Arash Goodarzi. Gelatin-based hydrogel functionalized with taurine moieties for in vivo skin tissue regeneration[J]. Journal of Zhejiang University Science D, 2023, 6(3): 284-297.

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Abstract: 
Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells (ECs) and are useful substrates for wound healing. The present study investigates the feasibility of covalent conjugation of taurine (Tau) on a gelatin-based hydrogel. This hydrogel is expected to maintain positive charged growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factors (VEGFs) near ECs within the hydrogel microenvironment. The gelatin was conjugated with hydroxyl phenol (Ph) and Tau moieties, and in following that Ph residues were crosslinked through a horseradish peroxidase-catalyzed reaction. The migration characteristics of ECs were analyzed by scratch migration assay and microparticle-based cell migration assay. Cellular morphology and amounts of angiopoietin 1 (Ang 1), bFGF, and VEGF proteins were evaluated for encapsulated cells. The potential of synthesized hydrogels in wound healing was assessed by the percentage of reduction from the original wound size and histopathological analyses of rat skin. The incorporated Tau molecules within the hydrogel remained stable through covalent bonds during incubation. During extended incubation, the gelatin-based hydrogel conjugated with Tau improved the migration distance and number of existing migrated ECs. Immobilized Tau within the gelatin-based hydrogel induced high motility of ECs, accompanied by robust cytoskeleton extension and a cell subpopulation that expressed CD44 and CD31 receptors as well as enhancement of Ang 1, bFGF, and VEGF. We found that injectable Gel-Ph-Tau effectively improves wound-healing parameters.

伊朗Arash Goodarzi等 | 用于体内皮肤组织再生的硫酸牛磺酸功能化明胶基水凝胶

本研究论文聚焦用于体内皮肤组织再生的硫酸牛磺酸功能化明胶基水凝胶。功能化水凝胶可刺激内皮细胞(ECs)的迁移和形态发生,是有用的促创伤愈合基质。本文研究牛磺酸(Tau)共价结合在基于明胶的水凝胶上的可行性。该水凝胶预计可以在水凝胶靠近ECs的微环境内维持带正电的生长因子,如碱性成纤维细胞生长因子(bFGF)和血管内皮细胞生长因子(VEGFs)。明胶通过羟基酚(Ph)和Tau官能团共价结合,随后通过辣根过氧化物酶催化反应交联Ph残基。通过划痕迁移实验和微粒细胞迁移实验分析ECs的迁移特性。对封装细胞的细胞形态和Ang 1、bFGF和VEGF蛋白的数量进行评估。通过原始创伤大小的降低百分比和大鼠皮肤组织病理学分析来评估合成水凝胶在创伤愈合中的潜力。在共培养期间,水凝胶中包含的Tau分子通过共价键稳定。随着共培养时间的延长,共价结合Tau的明胶基水凝胶提高了迁移距离和现有迁移ECs的数量。固定在基于明胶的水凝胶中的Tau诱导ECs高度运动性,伴随着强有力的细胞骨架延伸和表达CD44和CD31受体的细胞亚群,以及Ang 1、bFGF和VEGF的增强。我们发现,注射用Gel-Ph-Tau有效地改善了创伤愈合参数。

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