CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2023-12-13
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Amit Kumar Singh, Krishna Pramanik & Amit Biswas . Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological and mechanical properties for bone-tissue engineering[J]. Journal of Zhejiang University Science D, 2024, 7(1): 57-73.
@article{title="Constructing a biofunctionalized 3D-printed gelatin/sodium
alginate/chitosan tri-polymer complex scaffold with improvised
biological and mechanical properties for bone-tissue engineering",
author="Amit Kumar Singh, Krishna Pramanik & Amit Biswas ",
journal="Journal of Zhejiang University Science D",
volume="7",
number="1",
pages="57-73",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00249-z"
}
%0 Journal Article
%T Constructing a biofunctionalized 3D-printed gelatin/sodium
alginate/chitosan tri-polymer complex scaffold with improvised
biological and mechanical properties for bone-tissue engineering
%A Amit Kumar Singh
%A Krishna Pramanik & Amit Biswas
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 1
%P 57-73
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00249-z
TY - JOUR
T1 - Constructing a biofunctionalized 3D-printed gelatin/sodium
alginate/chitosan tri-polymer complex scaffold with improvised
biological and mechanical properties for bone-tissue engineering
A1 - Amit Kumar Singh
A1 - Krishna Pramanik & Amit Biswas
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 1
SP - 57
EP - 73
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00249-z
Abstract: sodium alginate (SA)/chitosan (CH) polyelectrolyte scaffold is a suitable substrate for tissue-engineering application. The
present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to
make it a potential template for bone-tissue regeneration. We experimented with adding 0%–15% (volume fraction) gelatin
(GE), a protein-based biopolymer known to promote cell adhesion, proliferation, and differentiation. The resulting tri-polymer
complex was used as bioink to fabricate SA/CH/GE matrices by three-dimensional (3D) printing. Morphological studies using
scanning electron microscopy revealed the microfibrous porous architecture of all the structures, which had a pore size range
of 383–419 μm. X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the
scaffold and the strong electrostatic interactions among the functional groups of the polymers, thereby forming polyelectrolyte
complexes which were found to improve mechanical properties and structural stability. The scaffolds exhibited a desirable
degradation rate, controlled swelling, and hydrophilic characteristics which are favorable for bone-tissue engineering. The
tensile strength improved from (386±15) to (693±15) kPa due to the increased stiffness of SA/CH scaffolds upon addition
of gelatin. The enhanced protein adsorption and in vitro bioactivity (forming an apatite layer) confirmed the ability of the
SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue
regeneration. In vitro biological evaluation including the MTT assay, confocal microscopy analysis, and alizarin red S assay
showed a significant increase in cell attachment, cell viability, and cell proliferation, which further improved biomineralization
over the scaffold surface. In addition, SA/CH containing 15% gelatin designated as SA/CH/GE15 showed superior performance
to the other fabricated 3D structures, demonstrating its potential for use in bone-tissue engineering
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