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On-line Access: 2025-03-01
Received: 2024-07-12
Revision Accepted: 2024-10-19
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Development of a multifunctional uniaxial bioreactor with real-time monitoring of culture conditions and tissue health
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Adit Mehta, Po-Feng Lee, Eric Renteria, Frank C. Marini, Ji Hyun Kim, Tracy Criswell, Thomas D. Shupe, Anthony Atala, Metin N. Gurcan, Shay Soker, Joshua Hunsberger, James J. Yoo, Young Min Ju. Development of a multifunctional uniaxial bioreactor with real-time monitoring of culture conditions and tissue health[J]. Journal of Zhejiang University Science D, 2025, 8(2): 310330.
@article{title="Development of a multifunctional uniaxial bioreactor with real-time monitoring of culture conditions and tissue health",
author="Adit Mehta, Po-Feng Lee, Eric Renteria, Frank C. Marini, Ji Hyun Kim, Tracy Criswell, Thomas D. Shupe, Anthony Atala, Metin N. Gurcan, Shay Soker, Joshua Hunsberger, James J. Yoo, Young Min Ju",
journal="Journal of Zhejiang University Science D",
volume="8",
number="2",
pages="310330",
year="2025",
publisher="Zhejiang University Press & Springer",
doi="10.1631/bdm.2400235"
}
%0 Journal Article
%T Development of a multifunctional uniaxial bioreactor with real-time monitoring of culture conditions and tissue health
%A Adit Mehta
%A Po-Feng Lee
%A Eric Renteria
%A Frank C. Marini
%A Ji Hyun Kim
%A Tracy Criswell
%A Thomas D. Shupe
%A Anthony Atala
%A Metin N. Gurcan
%A Shay Soker
%A Joshua Hunsberger
%A James J. Yoo
%A Young Min Ju
%J Journal of Zhejiang University SCIENCE D
%V 8
%N 2
%P 310330
%@ 1869-1951
%D 2025
%I Zhejiang University Press & Springer
%DOI 10.1631/bdm.2400235
TY - JOUR
T1 - Development of a multifunctional uniaxial bioreactor with real-time monitoring of culture conditions and tissue health
A1 - Adit Mehta
A1 - Po-Feng Lee
A1 - Eric Renteria
A1 - Frank C. Marini
A1 - Ji Hyun Kim
A1 - Tracy Criswell
A1 - Thomas D. Shupe
A1 - Anthony Atala
A1 - Metin N. Gurcan
A1 - Shay Soker
A1 - Joshua Hunsberger
A1 - James J. Yoo
A1 - Young Min Ju
J0 - Journal of Zhejiang University Science D
VL - 8
IS - 2
SP - 310330
EP -
%@ 1869-1951
Y1 - 2025
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/bdm.2400235
Abstract: Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation. Integrating sensors and imaging capabilities into bioreactors can help us understand how the culture environ? ment influences tissue maturation and growth. Additionally, this enables the monitoring of tissue constructs and provides critical information for quality control. This study aimed to develop a standardized, self-contained, uniaxial bioreactor mod? ule for the clinical manufacturing of tissue constructs; this system would benefit from unidirectional mechanical or electrical stimulation, or both. We achieved this goal by integrating stimulation and sensing components that provide an optimal cul? ture environment and monitoring capabilities to improve tissue manufacturing. The uniaxial bioreactor module included inte? grated, user-friendly mechanical and electrical stimulations with force measurement to enhance the preconditioning of the en? gineered tissues. Also, a sensor loop and media exchange system were integrated to monitor the culture environment and cel? lular metabolites over time, and the camera system above the tissue construct enabled the macroscopic visualization of tissue maturation. Furthermore, the onboard media exchange system was programmed into the module to maintain aseptic culture conditions in the long term. Subsequently, using native skeletal muscle tissue and tissue-engineered skeletal muscle con? structs, the performance of the uniaxial bioreactor module was validated for its application in preconditioning and enhancing tissue maturation.
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