Full Text:
<0>
CLC number:
On-line Access: 2025-05-20
Received: 2024-10-20
Revision Accepted: 2024-12-02
Crosschecked: 0000-00-00
Cited: 0
Clicked: 1
|
Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation
| |||||||||||||
Xiaofeng Gong (龚晓峰), Chen Yang (杨辰), Jianchen Peng (彭见晨), Xiao Ding (丁笑), Hui Yang (杨晖), Aaron Gerald Wang (王睿诚), Emmanuel Enoch Dzakah & Bing Zhao (赵冰). Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation[J]. Journal of Zhejiang University Science D, 2025, 8(3): 410–422.
@article{title="Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation",
author="Xiaofeng Gong (龚晓峰), Chen Yang (杨辰), Jianchen Peng (彭见晨), Xiao Ding (丁笑), Hui Yang (杨晖), Aaron Gerald Wang (王睿诚), Emmanuel Enoch Dzakah & Bing Zhao (赵冰)",
journal="Journal of Zhejiang University Science D",
volume="8",
number="3",
pages="410–422",
year="2025",
publisher="Zhejiang University Press & Springer",
doi="10.1631/bdm.2400424"
}
%0 Journal Article
%T Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation
%A Xiaofeng Gong (龚晓峰)
%A Chen Yang (杨辰)
%A Jianchen Peng (彭见晨)
%A Xiao Ding (丁笑)
%A Hui Yang (杨晖)
%A Aaron Gerald Wang (王睿诚)
%A Emmanuel Enoch Dzakah & Bing Zhao (赵冰)
%J Journal of Zhejiang University SCIENCE D
%V 8
%N 3
%P 410–422
%@ 1869-1951
%D 2025
%I Zhejiang University Press & Springer
%DOI 10.1631/bdm.2400424
TY - JOUR
T1 - Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation
A1 - Xiaofeng Gong (龚晓峰)
A1 - Chen Yang (杨辰)
A1 - Jianchen Peng (彭见晨)
A1 - Xiao Ding (丁笑)
A1 - Hui Yang (杨晖)
A1 - Aaron Gerald Wang (王睿诚)
A1 - Emmanuel Enoch Dzakah & Bing Zhao (赵冰)
J0 - Journal of Zhejiang University Science D
VL - 8
IS - 3
SP - 410–422
EP -
%@ 1869-1951
Y1 - 2025
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/bdm.2400424
Abstract: An organoid is a three-dimensional (3D) cell culture model that can reproduce the distinct structure and inherent functionality
of certain organs. Nevertheless, a major limitation of organoids is the absence of a complex vascular network, thus restricting
the supply of oxygen and essential nutrients. Coupled with their inherent size constraints and metabolite accumulation, it is
challenging for organoids to replicate the natural intricacies of organs, thereby limiting their applicability. To overcome the
challenges associated with this technology, we developed a culture platform to cultivate tumors or organ-derived organoids
up to the centimeter scale. Initially, a customized organoid-on-a-chip including a microvascular network at the micron scale
was designed using 3D printing. Further, by integrating an infusion device, the chip ensures an adequate supply of nutrients
and fluid immersion while mimicking blood flow dynamics. Our method overcomes the issue of the limited size of organ
oids due to insufficient nutrient access, making it possible to produce large-scale tumor and normal tissue models in vitro,
while providing insights into drug efficacy and toxicology evaluation as well as standardized organoid production.
Open peer comments: Debate/Discuss/Question/Opinion
<1>