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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.2 P.226-229

http://doi.org/10.1631/jzus.2004.0226


Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization


Author(s):  WU Yong-jiang, MA Ming-ming, ZENG Su

Affiliation(s):  College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China

Corresponding email(s):   zengsu@zjuem.zju.edu.cn

Key Words:  Enantioselective assay, Propafenone, Human urine, Chiral derivatization, High-performance liquid chromatography


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WU Yong-jiang, MA Ming-ming, ZENG Su. Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization[J]. Journal of Zhejiang University Science A, 2004, 5(2): 226-229.

@article{title="Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization",
author="WU Yong-jiang, MA Ming-ming, ZENG Su",
journal="Journal of Zhejiang University Science A",
volume="5",
number="2",
pages="226-229",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0226"
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%T Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization
%A WU Yong-jiang
%A MA Ming-ming
%A ZENG Su
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 2
%P 226-229
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0226

TY - JOUR
T1 - Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization
A1 - WU Yong-jiang
A1 - MA Ming-ming
A1 - ZENG Su
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 2
SP - 226
EP - 229
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0226


Abstract: 
The enantioselective assay for S(+)- and R(-)-propafenone (PPF) in human urine that developed in this work involves extraction of propafenone from human urine and using S(+)-propafenone as internal standard, chiral derivatization with 2,3,4,6-tetra-O-β-D-glucopranosyl isothiocyanate, and quantitation by an RP-HPLC system with UV detection (λ=220 nm). A baseline separation of propafenone enantiomers was achieved on a 5-μm reverse phase ODS column, with a mixture of methanol:water:glacial acetic acid (25:12:0.02,v/v) as mobile phase. There was good linear relationship from 24.9 ng/ml to 1875.0 ng/ml for both of enantiomers. The regression equations of the standard curves based on CS-PPF (or CR-PPF) versus ratio of AS-PPF/AS (or AR-PPF/AS) were y=0.0032x-0.081, (r=0.999) for S-PPF and y=0.0033x+0.0039, (r=0.998) for R-PPF, respectively. The method's limit of detection was 12.5 ng/ml for both enantiomers, and the method's limit of quantitation was 28.2±0.52 ng/ml for S-PPF, 30.4±0.53 ng/ml for R-PPF (RSD<8%, n=5). The analytical method yielded average recovery of 98.9% and 100.4% for S-PPF and R-PPF, respectively. The relative standard deviation was no more than 6.11% and 6.22% for S-PPF and R-PPF, respectively. The method enabled study of metabolism of S(+)- and R(-)-propafenone in human urine. The results from 7 volunteers administered 150 mg racemic propafenone indicated that propafenone enantiomers undergo stereoselective metabolism and that in the human body, S(+)-propafenone is metabolized more extensively than R(-)-propafenone.

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Reference

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