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CLC number: Q28

On-line Access: 2013-01-31

Received: 2012-06-04

Revision Accepted: 2013-01-15

Crosschecked: 2013-01-17

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.2 P.97-105


Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

Author(s):  Bing Feng, Lai-ji Ma, Jin-jing Yao, Yun Fang, Yan-ai Mei, Shao-min Wei

Affiliation(s):  School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China; more

Corresponding email(s):   weishaomin@jahwa.com.cn

Key Words:  Oat bran, Extraction, Antioxidant, Human dermal fibroblasts, Cell injury

Bing Feng, Lai-ji Ma, Jin-jing Yao, Yun Fang, Yan-ai Mei, Shao-min Wei. Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide[J]. Journal of Zhejiang University Science B, 2013, 14(2): 97-105.

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publisher="Zhejiang University Press & Springer",

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%T Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide
%A Bing Feng
%A Lai-ji Ma
%A Jin-jing Yao
%A Yun Fang
%A Yan-ai Mei
%A Shao-min Wei
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 2
%P 97-105
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200159

T1 - Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide
A1 - Bing Feng
A1 - Lai-ji Ma
A1 - Jin-jing Yao
A1 - Yun Fang
A1 - Yan-ai Mei
A1 - Shao-min Wei
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 2
SP - 97
EP - 105
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200159

Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investigation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide (H2O2). Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography (HPLC) analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1 301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity indicate that oat peptide-rich extract has a direct and concentration-dependent antioxidant activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay for apoptosis showed that administration of H2O2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H2O2, but application oat peptides with H2O2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H2O2-induced decrease of superoxide dismutase (SOD) and the inhibition of malondialdehyde (MDA). The results demonstrate that oat peptides possess antioxidant activity and are effective against H2O2-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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