CLC number: TS201.6
On-line Access:
Received: 2007-06-21
Revision Accepted: 2007-09-13
Crosschecked: 0000-00-00
Cited: 8
Clicked: 5954
Liu Yuan, Zhang Cun-zhen, Yu Xiang-yang, Zhang Zhi-yong, Zhang Xiao, Liu Rong-rong, Liu Xian-jin, Gong Zhen-ming. Development and evaluation of immunoassay for zeranol in bovine urine[J]. Journal of Zhejiang University Science B, 2007, 8(12): 900-905.
@article{title="Development and evaluation of immunoassay for zeranol in bovine urine",
author="Liu Yuan, Zhang Cun-zhen, Yu Xiang-yang, Zhang Zhi-yong, Zhang Xiao, Liu Rong-rong, Liu Xian-jin, Gong Zhen-ming",
journal="Journal of Zhejiang University Science B",
volume="8",
number="12",
pages="900-905",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0900"
}
%0 Journal Article
%T Development and evaluation of immunoassay for zeranol in bovine urine
%A Liu Yuan
%A Zhang Cun-zhen
%A Yu Xiang-yang
%A Zhang Zhi-yong
%A Zhang Xiao
%A Liu Rong-rong
%A Liu Xian-jin
%A Gong Zhen-ming
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 12
%P 900-905
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0900
TY - JOUR
T1 - Development and evaluation of immunoassay for zeranol in bovine urine
A1 - Liu Yuan
A1 - Zhang Cun-zhen
A1 - Yu Xiang-yang
A1 - Zhang Zhi-yong
A1 - Zhang Xiao
A1 - Liu Rong-rong
A1 - Liu Xian-jin
A1 - Gong Zhen-ming
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 12
SP - 900
EP - 905
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0900
Abstract: A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1:5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml , and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation (CVs) were in the range of 82%~127% and 3.5%~8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol (ranging from 0.2 to 10 μg/ml) were detected by the ELISA and liquid chromatography (LC) method, and good correlations were obtained between the two methods (R2=0.9643). We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an alternative for the conventional LC method for zeranol in bovine urine.
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