Similar articles

Abstract: Articular cartilage, which plays a vital role in joint structure, is susceptible to damage from trauma and degenerative joint diseases. Traditional methods for cartilage treatment often involve complex surgical procedures with limited efficacy. Alternatively, implantable drug-loaded scaffolds are an increasingly attractive cartilage treatment option. To address the challenges of structural and functional compatibility between scaffolds and native cartilage, as well as issues related to drug loading, we design a novel cartilage scaffold with a four-region hollow porous fiber network structure. Using an extrusion-based 3D printing platform, a biphasic silicone ink composed primarily of liquid-phase silicone and solid particles was employed to construct the hollow porous fiber network. Mechanical compression tests demonstrate that the cartilage scaffold has mechanical characteristics similar to those of native cartilage tissue, and ultraviolet spectrophotometry measurements confirm its ability to control drug release. These results showcase the feasibility and effectiveness of the proposed cartilage substitute structure.

仿生四区域药物负载软骨支架的设计与制造

[1]CaiJC, ChenY, LiuY, et al., 2022. Capillary imbibition and flow of wetting liquid in irregular capillaries: a 100-year review. Advances in Colloid and Interface Science, 304:102654.

[2]ChenPF, TaoJD, ZhuSA, et al., 2015. Radially oriented collagen scaffold with SDF-1 promotes osteochondral repair by facilitating cell homing. Biomaterials, 39:114-123.

[3]DaH, JiaSJ, MengGL, et al., 2013. The impact of compact layer in biphasic scaffold on osteochondral tissue engineering. PLoS One, 8(1):e54838.

[4]DuYY, LiuHM, YangQ, et al., 2017. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials, 137:37-48.

[5]GetgoodAMJ, KewSJ, BrooksR, et al., 2012. Evaluation of early-stage osteochondral defect repair using a biphasic scaffold based on a collagen-glycosaminoglycan biopolymer in a caprine model. The Knee, 19(4):422-430.

[6]GuZH, WangJY, FuY, et al., 2023. Smart biomaterials for articular cartilage repair and regeneration. Advanced Functional Materials, 33(10):2212561.

[7]GuiX, PengZ, SongP, et al., 2023. 3D printing of personalized polylactic acid scaffold laden with GelMA/autologous auricle cartilage to promote ear reconstruction. Bio-Design and Manufacturing, 6:451-463.

[8]HanG, LeeH, KangJM, et al., 2023. 3D-printed NIR-responsive bullets as multifunctional nanodrug platforms for image-guided local chemo-photothermal therapy. Chemical Engineering Journal, 477:147083.

[9]HuaYJ, HuoYY, BaiBS, et al., 2022. Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels. Materials Today Bio, 17:100489.

[10]KimSY, HanG, HwangDB, et al., 2021. Design and usability evaluations of a 3D-printed implantable drug delivery device for acute liver failure in preclinical settings. Advanced Healthcare Materials, 10(14):2100497.

[11]KimYH, KanczlerJM, LanhamS, et al., 2024. Biofabrication of nano-composite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells. Bio-Design and Manufacturing, 7:121-136.

[12]KwonH, BrownWE, LeeCA, et al., 2019. Surgical and tissue engineering strategies for articular cartilage and meniscus repair. Nature Reviews Rheumatology, 15(9):550-570.

[13]LevingstoneTJ, RameshA, BradyRT, et al., 2016a. Cell-free multi-layered collagen-based scaffolds demonstrate layer specific regeneration of functional osteochondral tissue in caprine joints. Biomaterials, 87:69-81.

[14]LevingstoneTJ, ThompsonE, MatsikoA, et al., 2016b. Multi-layered collagen-based scaffolds for osteochondral defect repair in rabbits. Acta Biomaterialia, 32:149-160.

[15]LiXS, ZhaoY, ZhaoC, 2021. Applications of capillary action in drug delivery. iScience, 24(7):102810.

[16]LiuH, ChengYL, ChenJJ, et al., 2018. Component effect of stem cell-loaded thermosensitive polypeptide hydrogels on cartilage repair. Acta Biomaterialia, 73:103-111.

[17]LiuJX, GaoYL, ZhangGR, et al., 2019. Follow-up study on autogenous osteochondral transplantation for cartilage defect of knee joint. China Journal of Orthopaedics and Traumatology, 32(4):346-349 (in Chinese).

[18]LiuJY, LiL, SuoHR, et al., 2019. 3D printing of biomimetic multi-layered GelMA/nHA scaffold for osteochondral defect repair. Materials & Design, 171:107708.

[19]MaldaJ, GrollJ, van WeerenPR, 2019. Rethinking articular cartilage regeneration based on a 250-year-old statement. Nature Reviews Rheumatology, 15(10):571-572.

[20]ParisiC, SalvatoreL, VeschiniL, et al., 2020. Biomimetic gradient scaffold of collagen-hydroxyapatite for osteochondral regeneration. Journal of Tissue Engineering, 11:2041731419896068.

[21]PattnaikA, SanketAS, PradhanS, et al., 2023. Designing of gradient scaffolds and their applications in tissue regeneration. Biomaterials, 296:122078.

[22]SchizasN, SavvidouO, TriantafyllopoulosI, et al., 2019. Adjuvant therapies for the enhancement of microfracture technique in cartilage repair. Orthopedic Reviews, 11(3):7950.

[23]ShenCY, WangJ, LiGF, et al., 2024. Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor. Bioactive Materials, 35:429-444.

[24]SinghAK, PramanikK, BiswasA, 2024. Constructing a biofunctional-ized 3D-printed gelatin/sodium alginate/chitosan tri-polymer com-plex scaffold with improvised biological and mechanical properties for bone-tissue engineering. Bio-Design and Manufacturing, 7:57-73.

[25]SoleymaniM, MotieeES, KarbasiS, et al., 2024. Evaluation of the effects of decellularized umbilical cord Wharton’s Jelly ECM on polyhydroxy butyrate electrospun scaffolds: a new strategy for cartilage tissue engineering. Materials Today Chemistry, 39:102145.

[26]SunZX, BonassarLJ, PutnamD, 2020. Influence of block length on articular cartilage lubrication with a diblock bottle-brush copolymer. ACS Applied Materials & Interfaces, 12(1):330-337.

[27]WangMK, WuY, LiGF, et al., 2024. Articular cartilage repair biomaterials: strategies and applications. Materials Today Bio, 24:100948.

[28]WangWZ, LiH, SongP, et al., 2024. Photo-crosslinked integrated triphasic scaffolds with gradient composition and strength for osteochondral regeneration. Journal of Materials Chemistry B, 12(5):1271-1284.

[29]WangZ, WangX, HuangY, et al., 2024. Cav3.3-mediated endochondral ossification in a three-dimensional bioprinted GelMA hydrogel. Bio-Design and Manufacturing, 7:983-999.

[30]YangHX, ZhengMJ, ZhangYY, et al., 2024. Enhanced angiogenesis in porous poly(ε-caprolactone) scaffolds fortified with methacrylated hyaluronic acid hydrogel after subcutaneous transplantation. Biomaterials Translational, 5(1):59-68.

[31]YangSH, WuHM, PengC, et al., 2024. From the microspheres to scaffolds: advances in polymer microsphere scaffolds for bone regeneration applications. Biomaterials Translational, 5(3):274-299.

[32]ZhangX, YanZH, GuanGT, et al., 2022. Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair. Journal of Biomaterials Applications, 36(6):1019-1032.

[33]ZhangYB, LiuXC, ZengLD, et al., 2019. Polymer fiber scaffolds for bone and cartilage tissue engineering. Advanced Functional Materials, 29(36):1903279.

[34]ZhouBT, XuPS, LiXZ, et al., 2005. Determination of gallium citrate injection by UV/VIS spectrophotometry. Central South Pharmacy, 3(1):30-31 (in Chinese).