Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering
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Peng-yan Qiao, Fang-fang Li, Li-min Dong, Tao Xu, Qiu-fei Xie. Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering[J]. Journal of Zhejiang University Science B, 2014, 15(4): 382-392.
@article{title="Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering",
author="Peng-yan Qiao, Fang-fang Li, Li-min Dong, Tao Xu, Qiu-fei Xie",
journal="Journal of Zhejiang University Science B",
volume="15",
number="4",
pages="382-392",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300132"
}
%0 Journal Article
%T Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering
%A Peng-yan Qiao
%A Fang-fang Li
%A Li-min Dong
%A Tao Xu
%A Qiu-fei Xie
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 4
%P 382-392
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300132
TY - JOUR
T1 - Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering
A1 - Peng-yan Qiao
A1 - Fang-fang Li
A1 - Li-min Dong
A1 - Tao Xu
A1 - Qiu-fei Xie
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 4
SP - 382
EP - 392
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300132
Abstract: Objective: To deliver cells deep into injectable calcium phosphate cement (CPC) through alginate-chitosan (AC) microcapsules and investigate the biological behavior of the cells released from microcapsules into the CPC. Methods: Mouse osteoblastic MC3T3-E1 cells were embedded in alginate and AC microcapsules using an electrostatic droplet generator. The two types of cell-encapsulating microcapsules were then mixed with a CPC paste. MC3T3-E1 cell viability was investigated using a Wst-8 kit, and osteogenic differentiation was demonstrated by an alkaline phosphatase (ALP) activity assay. Cell attachment in CPC was observed by an environment scanning electron microscopy. Results: Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC paste. The released cells attached to the setting CPC scaffolds, survived, differentiated, and formed mineralized nodules. Cells grew in the pores concomitantly created by the AC microcapsules in situ within the CPC. At Day 21, cellular ALP activity in the AC group was approximately four times that at Day 7 and exceeded that of the alginate microcapsule group (P<0.05). Pores formed by the AC microcapsules had a diameter of several hundred microns and were spherical compared with those formed by alginate microcapsules. Conclusions: AC microcapsule is a promising carrier to release seeding cells deep into an injectable CPC scaffold for bone engineering.
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CLC number: R318.08
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-03-19
Cited: 5
Clicked: 14206
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