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On-line Access: 2022-01-12

Received: 2021-02-16

Revision Accepted: 2021-06-03

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Shengbo SANG


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.1 P.58-73


Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering

Author(s):  Shengbo SANG, Rong CHENG, Yanyan CAO, Yayun YAN, Zhizhong SHEN, Yajing ZHAO, Yanqing HAN

Affiliation(s):  Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan 030024, China; more

Corresponding email(s):   sunboa-sang@tyut.edu.cn

Key Words:  Multi-walled carbon nanotube (MWCNT), Cell-scaffold, PC12 cells, Biocompatibility

Shengbo SANG, Rong CHENG, Yanyan CAO, Yayun YAN, Zhizhong SHEN, Yajing ZHAO, Yanqing HAN. Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering[J]. Journal of Zhejiang University Science B, 2022, 23(1): 58-73.

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author="Shengbo SANG, Rong CHENG, Yanyan CAO, Yayun YAN, Zhizhong SHEN, Yajing ZHAO, Yanqing HAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering
%A Shengbo SANG
%A Yanyan CAO
%A Yayun YAN
%A Zhizhong SHEN
%A Yajing ZHAO
%A Yanqing HAN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 1
%P 58-73
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100155

T1 - Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering
A1 - Shengbo SANG
A1 - Rong CHENG
A1 - Yanyan CAO
A1 - Yayun YAN
A1 - Zhizhong SHEN
A1 - Yajing ZHAO
A1 - Yanqing HAN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 1
SP - 58
EP - 73
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100155

Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings. CNT scaffolds are excellent mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility. CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration. The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs). CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying. Their physical and chemical properties and biocompatibility were evaluated. Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure. Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles. MWCNTs enhanced the elastic modulus of the scaffold. The porosity of the scaffolds ranged from 83% to 96%. They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration. The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content. Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells. We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins. Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.




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