Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification
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Hui-min Jia, Yu-tong Shen, Yun Jiao, Guo-yun Wang, Xiao Dong, Hui-juan Jia, Fang Du, Sen-miao Liang, Chao-chao Zhou, Wei-hua Mao, Zhong-shan Gao. Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification[J]. Journal of Zhejiang University Science B, 2014, 15(11): 997-1005.
@article{title="Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification",
author="Hui-min Jia, Yu-tong Shen, Yun Jiao, Guo-yun Wang, Xiao Dong, Hui-juan Jia, Fang Du, Sen-miao Liang, Chao-chao Zhou, Wei-hua Mao, Zhong-shan Gao",
journal="Journal of Zhejiang University Science B",
volume="15",
number="11",
pages="997-1005",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400051"
}
%0 Journal Article
%T Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification
%A Hui-min Jia
%A Yu-tong Shen
%A Yun Jiao
%A Guo-yun Wang
%A Xiao Dong
%A Hui-juan Jia
%A Fang Du
%A Sen-miao Liang
%A Chao-chao Zhou
%A Wei-hua Mao
%A Zhong-shan Gao
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 11
%P 997-1005
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400051
TY - JOUR
T1 - Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification
A1 - Hui-min Jia
A1 - Yu-tong Shen
A1 - Yun Jiao
A1 - Guo-yun Wang
A1 - Xiao Dong
A1 - Hui-juan Jia
A1 - Fang Du
A1 - Sen-miao Liang
A1 - Chao-chao Zhou
A1 - Wei-hua Mao
A1 - Zhong-shan Gao
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 11
SP - 997
EP - 1005
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400051
Abstract: chinese bayberry (Myrica rubra Sieb. et Zucc.) is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat (SSR) molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content (PIC) varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera (syn. Morella cerifera) and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between ‘Biqi’ and ‘Dongkui’. A neighbor-joining (NJ) tree was constructed to assess the genetic relationships among accessions, and the elite accessions ‘Y2010-70’, ‘Y2012-140’, and ‘Y2012-145’, were characterized as potential new genotypes for cultivation.
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CLC number: S667.6
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-10-15
Cited: 4
Clicked: 11900
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