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Abstract: Background: american ginseng (Panax quinquefolius L.) and chinese jujube (Zizyphus jujuba Mill.) are commonly used in traditional Chinese medicine to enhance immune function. Objective: The present study aimed to develop one Chinese prescription, Shenzao Cha (SZC), consisting of american ginseng and chinese jujube, and systematically investigate its immunomodulation in healthy ICR mice. Methods: Normal ICR mice received intragastric administration of SZC (1.3, 2.6, and 5.2 g raw material/kg body weight) once daily for four weeks, while a control group received the same amount of sterile water. Results: SZC significantly increased the spleen and thymus indices and T-lymphocyte proliferation, while the T-lymphocyte proliferation in the 5.2 g/kg group was 1.4-fold higher than that in the control. Further, 1.3 g/kg SZC could markedly improve hemolytic activity by 25.2%, and 2.6 g/kg SZC increased the NK cell activity by 78.6% relative to the control. In addition, the activities of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase), that participated in modulating oxidative stress, were significantly increased in the liver, spleen, thymus, and serum, while the contents of malondialdehyde were dramatically decreased. Conclusions: SZC exhibited potent immunomodulatory effects on innate and adaptive immunity in healthy ICR mice, as well as potential antioxidant activity for prevention of oxidative stress, which was suggested to partly contribute to the immune enhancement.

复方西洋参和红枣制剂对小鼠免疫功能影响的研究

[1]Barton, D.L., Liu, H., Dakhil, S.R., et al., 2013. Wisconsin Ginseng (Panax quinquefolius) to improve cancer-related fatigue: a randomized, double-blind trial, N07C2. JNCI, 105(16):1230-1238.

[2]Chen, Y., Tang, J., Wang, X., et al., 2012. An immunostimulatory polysaccharide (SCP-IIa) from the fruit of Schisandra chinensis (Turcz.) Baill. Int. J. Biol. Macromol., 50(3):844-848.

[3]D'Autreaux, B., Toledano, M.B., 2007. ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nat. Rev. Mol. Cell Biol., 8(10):813-824.

[4]de la Fuente, M., Victor, V.M., 2000. Anti-oxidants as modulators of immune function. Immunol. Cell Biol., 78(1):49-54.

[5]Dietert, R.R., Zelikoff, J.T., 2010. Identifying patterns of immune-related disease: use in disease prevention and management. World J. Pediatr., 6(2):111-118.

[6]Durairaj, P., Breda, M., Miller, S.C., 2013. Quantitative augmentation of immune cells in elderly normal mice by short-term, daily consumption of an extract of North American ginseng (Panax quinquefolius). Biomed. Res., 24(2):199-205.

[7]Fan, Y., Ma, L., Zhang, W., et al., 2014. The design of propolis flavone microemulsion and its effect on enhancing the immunity and antioxidant activity in mice. Int. J. Biol. Macromol., 65:200-207.

[8]Gao, Q.H., Wu, C.S., Wang, M., et al., 2013. The Jujube (Ziziphus Jujuba Mill.) Fruit: a review of current knowledge of fruit composition and health benefits. J. Agric. Food Chem., 61(14):3351-3363.

[9]Gil, A., Rueda, R., 2002. Interaction of early diet and the development of the immune system. Nutr. Res. Rev., 15(2):263-292.

[10]Goldstone, S.D., Hunt, N.H., et al., 1997. Redox regulation of the mitogen-activated protein kinase pathway during lymphocyte activation. BBA-Mol. Cell. Res., 1355(3):353-360.

[11]Gunzer, W., Konrad, M., Pail, E., et al., 2012. Exercise-induced immunodepression in endurance athletes and nutritional intervention with carbohydrate, protein and fat—what is possible, what is not Nutrients, 4(9):1187-1212.

[12]Huang, G.C., Wu, L.S., Chen, L.G., et al., 2007. Immuno-enhancement effects of Huang Qi Liu Yi Tang in a murine model of cyclophosphamide-induced leucopenia. J. Ethnopharmacol., 109(2):229-235.

[13]Ichikawa, T., Li, J., Nagarkatti, P., et al., 2009. American ginseng preferentially suppresses STAT/iNOS signaling in activated macrophages. J. Ethnopharmacol., 125(1):145-150.

[14]Jiang, C.X., Zhao, L., Li, S.L., et al., 2013. Preliminary characterization and immunostimulatory activity of polysaccharides from Glossaulax didyma. Food Chem. Toxicol., 62:226-230.

[15]Kim, D.H., 2012. Chemical diversity of Panax ginseng, Panax quinquifolium, and Panax notoginseng. J. Ginseng Res., 36(1):1-15.

[16]Kim, J.E., Cho, H.S., Yang, H.S., et al., 2012. Depletion of ascorbic acid impairs NK cell activity against ovarian cancer in a mouse model. Immunobiology, 217(9):873-881.

[17]Kim, K.T., Yoo, K.M., Lee, J.W., et al., 2007. Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharmacol., 111(3):443-450.

[18]Knight, J.A., 2000. Review: free radicals, antioxidants and the immune system. Ann. Clin. Lab. Sci., 30(2):145-158.

[19]LeMaoult, J., Szabo, P., Weksler, M.E., 1997. Effect of age on humoral immunity, selection of the B-cell repertoire and B-cell development. Immunol. Rev., 160(1):115-126.

[20]Lemmon, H.R., Sham, J., Chau, L.A., et al., 2012. High molecular weight polysaccharides are key immunomodulators in North American ginseng extracts: characterization of the ginseng genetic signature in primary human immune cells. J. Ethnopharmacol., 142(1):1-13.

[21]Lesser, M.P., Shick, J.M., 1989. Effects of irraniance and ultraviolet-radiation on photodaptation in the zooxanthellae of Aiptasia pallida: primary production, photoinhibition, and enzymic defenses against oxygen-toxicity. Mar. Biol., 102(2):243-255.

[22]Li, J.Q., Ichikawa, T., Jin, Y., et al., 2010. An essential role of Nrf2 in American ginseng-mediated anti-oxidative actions in cardiomyocytes. J. Ethnopharmacol., 130(2):222-230.

[23]Li, J.W., Ding, S.D., Ding, X.L., 2007. Optimization of the ultrasonically assisted extraction of polysaccharides from Zizyphus jujuba cv. jinsixiaozao. J. Food Eng., 80(1):176-183.

[24]Li, W.J., Nie, S.P., Peng, X.P., et al., 2012. Ganoderma atrum polysaccharide improves age-related oxidative stress and immune impairment in mice. J. Agric. Food Chem., 60(6):1413-1418.

[25]Lv, Y.J., Huang, J.M., Cai, M., et al., 2013. A health food high-peptide meal alleviates immunosuppression induced by hydrocortisone and cyclophosphamide in mice. Food Funct., 4(9):1352-1359.

[26]Morris, A.C., Anderson, N., Brittan, M., et al., 2013. Combined dysfunctions of immune cells predict nosocomial infection in critically ill patients. Br. J. Anaesth., 111(5):778-787.

[27]Mucalo, I., Jovanovski, E., Rahelic, D., et al., 2013. Effect of American ginseng (Panax quinquefolius L.) on arterial stiffness in subjects with type-2 diabetes and concomitant hypertension. J. Ethnopharmacol., 150(1):148-153.

[28]Oshimi, K., 2007. Progress in understanding and managing natural killer-cell malignancies. Brit. J. Haematol., 139(4):532-544.

[29]Plastina, P., Bonofiglio, D., Vizza, D., et al., 2012. Identification of bioactive constituents of Ziziphus jujube fruit extracts exerting antiproliferative and apoptotic effects in human breast cancer cells. J. Ethnopharmacol., 140(2):325-332.

[30]Qi, L.W., Wang, C.Z., Yuan, C.S., 2011. Ginsenosides from American ginseng: chemical and pharmacological diversity. Phytochemistry, 72(8):689-699.

[31]Salva, S., Cecilia Merino, M., Agueero, G., et al., 2012. Dietary supplementation with probiotics improves hematopoiesis in malnourished mice. PLoS ONE, 7(2):e31171.

[32]See, D.M., Broumand, N., Sahl, L., et al., 1997. In vitro effects of echinacea and ginseng on natural killer and antibody-dependent cell cytotoxicity in health subjects and chronic fatigue syndrome or acquired immunodeficiency syndrome patients. Immunopharmacology, 35(3):229-235.

[33]Shen, X., Tang, Y., Yang, R., et al., 2009. The protective effect of Zizyphus jujube fruit on carbon tetrachloride-induced hepatic injury in mice by anti-oxidative activities. J. Ethnopharmacol., 122(3):555-560.

[34]Sordillo, L.M., Aitken, S.L., 2009. Impact of oxidative stress on the health and immune function of dairy cattle. Vet. Immunol. Immunop., 128(1-3):104-109.

[35]Srikumar, R., Parthasarathy, N.J., Manikandan, S., et al., 2006. Effect of Triphala on oxidative stress and on cell-mediated immune response against noise stress in rats. Mol. Cell. Biochem., 283(1-2):67-74.

[36]Tian, Z., Gershwin, M.E., Zhang, C., 2012. Regulatory NK cells in autoimmune disease. J. Autoimmun., 39(3):206-215.

[37]Wang, B., 2011. Chemical characterization and ameliorating effect of polysaccharide from Chinese jujube on intestine oxidative injury by ischemia and reperfusion. Int. J. Biol. Macromol., 48(3):386-391.

[38]Wang, D., Zhao, Y., Jiao, Y., et al., 2012. Antioxidative and hepatoprotective effects of the polysaccharides from Zizyphus jujube cv. Shaanbeitanzao. Carbohyd. Polym., 88(4):1453-1459.

[39]Wang, J.X., Tong, X., Li, P.B., et al., 2012. Immuno-enhancement effects of Shenqi Fuzheng injection on cyclophosphamide-induced immunosuppression in Balb/c mice. J. Ethnopharmacol., 139(3):788-795.

[40]Wu, D., Pae, M., Ren, Z., et al., 2007. Dietary supplementation with white button mushroom enhances natural killer cell activity in C57BL/6 mice. J. Nutr., 137(6):1472-1477.

[41]Xie, Z., Huang, H., Zhao, Y., et al., 2012. Chemical composition and anti-proliferative and anti-inflammatory effects of the leaf and whole-plant samples of diploid and tetraploid Gynostemma pentaphyllum (Thunb.) Makino. Food Chem., 132(1):125-133.

[42]Yang, H.Y., Liu, S.L., Ibrahim, S.A., et al., 2009. Oral administration of live Bifidobacterium substrains isolated from healthy centenarians enhanced immune function in BALB/c mice. Nutr. Res., 29(4):281-289.

[43]Yen, M.H., Lee, J.J., Yeh, C.F., et al., 2014. Yakammaoto inhibited human coxsackievirus B4 (CVB4)-induced airway and renal tubular injuries by preventing viral attachment, internalization, and replication. J. Ethnopharmacol., 151(3):1056-1063.

[44]Yuan, C.S., Attele, A.S., Wu, J.A., et al., 1998. Modulation of American ginseng on brainstem GABAergic effects in rats. J. Ethnopharmacol., 62(3):215-222.

[45]Yuan, C.S., Wang, C.Z., Wicks, S.M., et al., 2010. Chemical and pharmacological studies of saponins with a focus on American ginseng. J. Ginseng Res., 34(3):160-167.

[46]Zhang, J., Chen, J., Wang, D., et al., 2013. Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide. Int. J. Biol. Macromol., 52:212-217.

[47]Zhao, Z., Li, J., Wu, X., et al., 2006. Structures and immunological activities of two pectic polysaccharides from the fruits of Ziziphus jujuba Mill. cv. jinsixiaozao Hort. Food Res. Int., 39(8):917-923.

[48]Zhao, Z., Liu, M., Tu, P., et al., 2008. Characterization of water soluble polysaccharides from organs of Chinese Jujube (Ziziphus jujuba Mill. cv. Dongzao). Eur. Food Res. Technol., 226(5):985-989.

[49]Zhu, X.L., Chen, A.F., Lin, Z.B., 2007. Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. J. Ethnopharmacol., 111(2):219-226.