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Bio-Design and Manufacturing  2021 Vol.4 No.3 P.452-468

http://doi.org/10.1007/s42242-021-00130-x


A bifunctional bone scaffold combines osteogenesis and antibacterial activity via in situ grown hydroxyapatite and silver nanoparticles


Author(s):  Youwen Yang, Yun Cheng, Fang Deng, Lida Shen, Zhenyu Zhao, Shuping Peng & Cijun Shuai

Affiliation(s):  Institute of Bioadditive Manufacturing, Jiangxi University of Science and Technology, Nanchang 330013, China; more

Corresponding email(s):   shuping@csu.edu.cn, shuai@csu.edu.cn

Key Words:  Bone scaffold, In situ growth, Hydroxyapatite, Antibacterial properties, Surface modification


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Youwen Yang, Yun Cheng, Fang Deng, Lida Shen, Zhenyu Zhao, Shuping Peng & Cijun Shuai. A bifunctional bone scaffold combines osteogenesis and antibacterial activity via in situ grown hydroxyapatite and silver nanoparticles[J]. Journal of Zhejiang University Science D, 2021, 4(3): 452-468.

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Abstract: 
hydroxyapatite (HA) nanoparticles and silver (Ag) nanoparticles are expected to enable desirable bioactivity and antibacterial properties on biopolymer scaffolds. Nevertheless, interfacial adhesion between HA/Ag and the biopolymer is poor due to the large physicochemical differences between these components. In this study, poly L-lactic acid (PLLA) powder was first surface-modified with bioactive polydopamine (PDA) in an alkaline environment. Next, HA and Ag nanoparticles were grown in situ on the PDA-coated PLLA powder, which was then adhered to the porous bone scaffold using a selective laser-sintering process. Results showed that HA and Ag nanoparticles were homogenously distributed in the matrix, with enhanced mechanical properties. Simulated body fluid bioactivity tests showed that the in situ grown HA-endowed scaffold shows excellent bioactivity. In vitro tests confirmed that the scaffold exhibits favorable biocompatibility with human umbilical cord mesenchymal stem cells, as well as strong antibacterial activity against Gram-negative Escherichia coli. Furthermore, in vivo assays indicated that the scaffold promoted bone generation, with a new bone area fraction of 71.8% after 8 weeks’ implantation, without inflammation.

中南大学帅词俊等 | 羟基磷灰石和银纳米粒子原位生长合成具有促成骨与抗菌能力的双功能支架

本研究论文关注成骨修复相关研究。羟基磷灰石(HA)纳米粒子和银纳米粒子有望在生物高分子支架上实现理想的生物活性和抗菌性能。然而,HA/银与生物高分子之间理化学差异较大,因此界面粘附性很差。在这项研究中,左旋聚乳酸(PLLA)粉末首先在碱性环境中用具有良好生物活性的聚多巴胺(PDA)进行表面修饰。随后,HA和银纳米粒子原位生长在PLLA粉末的PDA涂层上,然后使用选择性激光烧结工艺制备多孔骨支架。结果表明,HA和银纳米粒子均匀分布在高分子基体中,使其机械性能得到增强。模拟体液测试表明,原位生长的HA使支架展现出优异的生物活性。体外测试证实,骨支架具有与人类脐带间充质干细胞良好的生物相容性,以及对革兰阴性大肠杆菌的强抗菌活性。此外,体内测试表明,骨支架可以促进新骨生成,植入8周后新骨面积比例为71.8%,且没有炎症发生。

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