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Suppl. Mater.: 

CLC number: O658.2

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2016-08-18

Cited: 0

Clicked: 5886

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhari Ismail

http://orcid.org/0000-0002-0862-3507

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.9 P.683-691

http://doi.org/10.1631/jzus.B1600019


A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth


Author(s):  Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid

Affiliation(s):  Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; more

Corresponding email(s):   ismailzhari@gmail.com

Key Words:  Syzygium campanulatum, Supercritical fluid extraction, Conventional solvent extractions, Flavanones, Chalcone, Triterpenoids



Abstract: 
Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids). In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques (maceration, soxhletion, and reflux). An enriched extract of five secondary metabolites was collected using n-hexane:methanol (1:1) soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery (25.5%‒84.9%) of selected secondary metabolites as compared to the CSE techniques (0.92%‒66.00%).

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