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Received: 2005-03-01

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.10 P.999-1004


Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.)

Author(s):  HE Guo-qing, XIONG Hao-ping, CHEN Qi-he, RUAN Hui, WANG Zhao-yue, TRAORÉ, Lonseny

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   gqhe@zju.edu.cn, hpxiong@126.com

Key Words:  Optimization, Supercritical fluid extraction, Flavonoids, Xanthohumol, Hops (Humulus lupulus L.)

HE Guo-qing, XIONG Hao-ping, CHEN Qi-he, RUAN Hui, WANG Zhao-yue, TRAORÉ Lonseny. Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.)[J]. Journal of Zhejiang University Science B, 2005, 6(10): 999-1004.

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journal="Journal of Zhejiang University Science B",
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%A HE Guo-qing
%A XIONG Hao-ping
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%A WANG Zhao-yue
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T1 - Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.)
A1 - HE Guo-qing
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A1 - RUAN Hui
A1 - WANG Zhao-yue
A1 - Lonseny
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B0999

Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO2 extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of ethanol (modifier) and the ratio (w/w) of solvent to material were tested in this study. The results of single factor and orthogonal experiments showed that at 50 °C, 25 MPa, the ratio of solvent to material (50%), ethanol concentration (80%) resulted in maximum extraction yield flavonoids (7.8 mg/g). HPLC-MS analysis of the extracts indicated that flavonoids obtained were xanthohumol, the principal prenylflavonoid in hops.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Anna, M., Marina, K., Mihkel, K., Anne, O., 2004. Identification and characterization of supercritical fluid extracts from herbs. C. R. Chimie, 7:629-633.

[2] Catchpole, O.J., Grey, J.B., Mitchell, K.A., Lan, J.S., 2004. Supercritical antisolvent fractionation of propolis tincture. Journal of Supercritical Fluids, 29:97-106.

[3] Chiu, K.L., Cheng, Y.C., Chen, J.H., Chang, C.J., Yang, P.W., 2002. Supercritical fluids extraction of Ginkgo ginkgolides and flavonoids. Journal of Supercritical Fluids, 24:77-87.

[4] de Maria, L.L.M., Janete, H.Y.V., Fernando, M.L., 1997. Supercritical fluid extraction of glycosylated flavonoids from Passiflora leaves. Phytochemical Analysis, 8:257-260.

[5] Gardner, D.S., 1993. Commercial Scale Extraction of Alpha-Acids and Hop Oils with Compressed CO2. In: King, M.B., Bott, T.R. (Eds.), Extraction of Natural Products Using Near-Critical Solvents. Blackie Academic and Professional, London, UK, p.84-100.

[6] Henderson, M.C., Miranda, C.L., Stevens, J.F., Deinzer, M.L., Buhler, D.R., 1998. In vitro inhibition of carcinogen metabolism by flavonoids from hops (Humulus lupulus) in various species. The Toxicologist, 42:185.

[7] Jose, M., Oscar, R., Osvaldo, T., Palma, M.T., 2003. Supercritical CO2 extraction of Chilean hop (Humulus lupulus) ecotypes. Journal of the Science of Food and Agriculture, 83:1349-1356.

[8] Laws, D.R., Bath, N.A., Pickett, J.A., Ennis, C.S., Wheldon, A.G., 1977. Preparation of hop extracts without using organic solvents. Journal of the Institute of Brewing, 83:39-40.

[9] Li, Q.S., Zhang, Z.T., Zhong, C.L., Liu, Y.C., Zhou, Q.R., 2003. Solubility of solid solutes in supercritical carbon dioxide with and without cosolvents. Fluid Phase Equilibria, 207:183-192.

[10] Lin, M.C., Tsai, M.J., Wen, K.C., 1999. Supercritical fluid extraction of flavonoids from Scutellariae Radix. Journal of Chromatography A, 830:387-395.

[11] Luque de Castro, M.D., Tena, M.T., 1996. Strategies for supercritical fluid extraction of polar and ionic compounds. Trends in Analytical Chemistry, 15:32-37.

[12] Milligan, S.R., Kalita, J.C., Pocock, V., Kauter, V., Stevens, J.F., Deinzer, M.L., Rong, H., Keukeleire, D., 2000. The endocrine activities of 8-prenylnaringenin and related hop (Humulus lupulus L.) flavonoids. Journal of Clinical Endocrinology and Metabolism, 85:4912-4915.

[13] Miranda, C.L., Stevens, J.F., Helmrich, A., Henderson, M.C., Rodriguez, R.J., Deinzer, M.L., Barnes, D.W., Buhler, D.R., 1999. Antiproliferative and cytotoxic effects of prenylated flavonoids from hops (Humulus lupulus) in human cancer cell lines. Food and Chemical Toxicology, 37:271-285.

[14] Miranda, C.L., Aponso, L., Stevens, J.F., Deinzer, M.L., Buhler, D.R., 2000. Prenylated chalcones and flavanones as inducers of quinone reductase in mouse hepatoma (Hepa lclc7) cells. Cancer Letters, 149:21-39.

[15] Mizobuchi, S., Sato, Y., 1984. A new flavanone with antifungal activity isolated from hops. Agriculture Biology Chemistry, 48:2771-2775.

[16] Palmer, M.V., Ting, S.S.T., 1995. Applications for supercritical fluid technology in food processing. Food Chemistry, 52:345-352.

[17] Stevens, J.F., Page, J.E., 2004. Xanthohumol and related prenylflavonoids from hops and beer: to your good health. Phytochemistry, 65:1317-1330.

[18] Stevens, J.F., Monika, I., Victor, L.H., Deinzer, M.L., 1997. Prenylflavoniods from Humulus lupulus. Phytochemistry, 44:1575-1585.

[19] Stevens, J.F., Miranda, C.L., Buhler, D.R., Deinzer, M.L., 1998. Chemistry and biology of hop flavonoids. Journal of the American Society of Brewing Chemists, 56:136-145.

[20] Stevens, J.F., Taylor, A.W., Deinzer, M.L., 1999. Quantitative analysis of xanthohumol and related prenylflavonoids in hops and beer by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 832:97-107.

[21] Tabata, N., Ito, M., Tomoda, H., Omura, S., 1997. Xanthohumols, diacylycerol acyltransrerase inhibitors, from Humulus lupulus. Phytochemistry, 46:683-687.

[22] Tobe, H., Muraki, Y., Kitamura, K., Komiyama, O., Sato, Y., Sugioka, T., Maruyama, H.B., Matsuda, E., Nagai, M., 1997. Bone resorption inhibitors from hop extract. Bioscience Biotechnology Biochemistry, 61:158-159.

[23] Tong, P., Imagawa, T., 1995. Optimization of supercritical fluid extraction for polychlorinated biphenyls from sediments. Analytica Chimica Acta, 310:93-100.

[24] van der Velde, E.G., Dietvorst, M., Swart, C.P., Pamlal, M.R., Kootstra, P.R., 1994. Optimization of supercritical fluid extraction of organochlorine pesticides from real soil samples. Journal of Chromatography A, 683:167-174.

[25] Zhuang, X.P., Yu, X.Y., 1992. Determination of total flavonoids in the leaves of Ginkgo and studies on its extraction process. Chinese Traditional and Herbal Drugs, 23:122-124 (in Chinese).

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