Full Text:
<772>
Summary: 
<451>
Suppl. Mater.: 
CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2023-08-08
Cited: 0
Clicked: 950
Citations: Bibtex RefMan EndNote GB/T7714
Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
| ||||||||||||||
Fang WU, Chen LU, Wenhao HU, Xin GUO, Jiayue CHEN, Zhidan LUO. Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination[J]. Journal of Zhejiang University Science B, 2023, 24(8): 749-754.
@article{title="Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination",
author="Fang WU, Chen LU, Wenhao HU, Xin GUO, Jiayue CHEN, Zhidan LUO",
journal="Journal of Zhejiang University Science B",
volume="24",
number="8",
pages="749-754",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200705"
}
%0 Journal Article
%T Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
%A Fang WU
%A Chen LU
%A Wenhao HU
%A Xin GUO
%A Jiayue CHEN
%A Zhidan LUO
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 8
%P 749-754
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200705
TY - JOUR
T1 - Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
A1 - Fang WU
A1 - Chen LU
A1 - Wenhao HU
A1 - Xin GUO
A1 - Jiayue CHEN
A1 - Zhidan LUO
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 8
SP - 749
EP - 754
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200705
Abstract: vibrio parahaemolyticus is a major pathogen frequently found in seafood. Rapid and accurate detection of this pathogen is important for the control of bacterial foodborne diseases and to ensure food safety. In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.
[1]GaoH, ShangZ, ChanSY, et al., 2022. Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 23(11):881-898.
[2]HeY, WangH, ChenLM, 2015. Comparative secretomics reveals novel virulence-associated factors of Vibrio parahaemolyticus. Front Microbiol, 6:707.
[3]HsiehK, MagePL, CsordasAT, et al., 2014. Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Chem Commun (Camb), 50(28):3747-3749.
[4]LanesO, LeirosI, SmalåsAO, et al., 2002. Identification, cloning, and expression of uracil-DNA glycosylase from Atlantic cod (Gadus morhua): characterization and homology modeling of the cold-active catalytic domain. Extremophiles, 6(1):73-86.
[5]LiLX, LiSY, WuN, et al., 2019. HOLMESv2: a CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation. ACS Synth Biol, 8(10):2228-2237.
[6]LuSH, TongXH, HanY, et al., 2022. Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a. Nat Biomed Eng, 6(3):286-297.
[7]LvXR, CaoWW, ZhangH, et al., 2022. CE-RAA-CRISPR assay: a rapid and sensitive method for detecting Vibrio parahaemolyticus in seafood. Foods, 11(12):1681.
[8]NemotoJ, IkedoM, KojimaT, et al., 2011. Development and evaluation of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Vibrio parahaemolyticus. J Food Prot, 74(9):1462-1467.
[9]SuYC, LiuCC, 2007. Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol, 24(6):549-558.
[10]TangY, ChenH, DiaoYX, 2016. Advanced uracil DNA glycosylase-supplemented real-time reverse transcription loop-mediated isothermal amplification (UDG-rRT-LAMP) method for universal and specific detection of Tembusu virus. Sci Rep, 6:27605.
[11]TengF, GuoL, CuiTT, et al., 2019. CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity. Genome Biol, 20:132.
[12]WardLN, BejAK, 2006. Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes. Appl Environ Microbiol, 72(3):2031-2042.
[13]YangXH, ZhaoPP, DongY, et al., 2020. An improved recombinase polymerase amplification assay for visual detection of Vibrio parahaemolyticus with lateral flow strips. J Food Sci, 85(6):1834-1844.
[14]ZhangT, ZhaoW, ZhaoW, et al., 2021. Universally stable and precise CRISPR-LAMP detection platform for precise multiple respiratory tract virus diagnosis including mutant SARS-CoV-2 spike N501Y. Anal Chem, 93(48):16184-16193.
[15]ZhangZH, LouY, DuSP, et al., 2017. Prevalence of Vibrio parahaemolyticus in seafood products from hypermarkets in Shanghai. J Sci Food Agric, 97(2):705-710.
ORCID: 
Open peer comments: Debate/Discuss/Question/Opinion
<1>