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On-line Access: 2024-12-30
Received: 2023-09-03
Revision Accepted: 2024-03-04
Crosschecked: 2024-12-30
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Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack
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Yuanyuan XIE, Xiaoyan YANG, Rong PAN, Lixia GAO, Ling YU. Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack[J]. Journal of Zhejiang University Science B, 2024, 25(12): 1097-1107.
@article{title="Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack",
author="Yuanyuan XIE, Xiaoyan YANG, Rong PAN, Lixia GAO, Ling YU",
journal="Journal of Zhejiang University Science B",
volume="25",
number="12",
pages="1097-1107",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300617"
}
%0 Journal Article
%T Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack
%A Yuanyuan XIE
%A Xiaoyan YANG
%A Rong PAN
%A Lixia GAO
%A Ling YU
%J Journal of Zhejiang University SCIENCE B
%V 25
%N 12
%P 1097-1107
%@ 1673-1581
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300617
TY - JOUR
T1 - Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack
A1 - Yuanyuan XIE
A1 - Xiaoyan YANG
A1 - Rong PAN
A1 - Lixia GAO
A1 - Ling YU
J0 - Journal of Zhejiang University Science B
VL - 25
IS - 12
SP - 1097
EP - 1107
%@ 1673-1581
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300617
Abstract: Multilayer paper-based cell culture, as an in vitro three-dimensional (3D) cell culture method, has been frequently used to research drug bioavailability, therapeutic efficacy, and dose-limiting toxicity in malignant tumors. This paper proposes a heterogenous multilayer paper stacking co-culture system to establish a model of natural killer (NK) cells moving through the endothelium layer and attacking tumor spheroids. This system consists of three layers: a bottom tumor-spheroid layer, a middle invasion layer, and a top endothelium layer. NK-92 cells were placed in the supernatant on top of the three layers. After two days of co-culture, the attack of tumor spheroids by NK cells was observed. We additionally examined the infiltration of NK-92 cells within the tumor spheroids at different Z-axis depths using a confocal microscope, and the results suggested that the system successfully realizes NK cells traveling cross the endothelium layer to form tumor-infiltrating NK cells (TINKs). The potential application of multilayer paper for co-culture models involving cancer cells and immune cells holds great promise for exploring the interaction dynamics of these two cell types.
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