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Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route
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Yapeng YANG1,2,3, Xiang TAN2, Zeyue ZHANG2, Lifeng LIANG4, Zhifeng WU2, Jinhui HE2, Yuqing WANG2,3, Miaomiao DONG2, Jixia ZHENG2, Hang ZHANG2,5, Shuaifei FENG2, Wei CHENG2, Bota CUI6, Hong WEI2,5, Qinjin LI1. Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route",
author="Yapeng YANG1,2,3, Xiang TAN2, Zeyue ZHANG2, Lifeng LIANG4, Zhifeng WU2, Jinhui HE2, Yuqing WANG2,3, Miaomiao DONG2, Jixia ZHENG2, Hang ZHANG2,5, Shuaifei FENG2, Wei CHENG2, Bota CUI6, Hong WEI2,5, Qinjin LI1",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2400495"
}
%0 Journal Article
%T Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route
%A Yapeng YANG1
%A 2
%A 3
%A Xiang TAN2
%A Zeyue ZHANG2
%A Lifeng LIANG4
%A Zhifeng WU2
%A Jinhui HE2
%A Yuqing WANG2
%A 3
%A Miaomiao DONG2
%A Jixia ZHENG2
%A Hang ZHANG2
%A 5
%A Shuaifei FENG2
%A Wei CHENG2
%A Bota CUI6
%A Hong WEI2
%A 5
%A Qinjin LI1
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2400495
TY - JOUR
T1 - Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route
A1 - Yapeng YANG1
A1 - 2
A1 - 3
A1 - Xiang TAN2
A1 - Zeyue ZHANG2
A1 - Lifeng LIANG4
A1 - Zhifeng WU2
A1 - Jinhui HE2
A1 - Yuqing WANG2
A1 - 3
A1 - Miaomiao DONG2
A1 - Jixia ZHENG2
A1 - Hang ZHANG2
A1 - 5
A1 - Shuaifei FENG2
A1 - Wei CHENG2
A1 - Bota CUI6
A1 - Hong WEI2
A1 - 5
A1 - Qinjin LI1
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2400495
Abstract: Human flora-associated (HFA) mice are often used to simulate the structure of human intestinal microbiota and to study the causal relationship between diseases and gut microbiota. However, several factors affect the colonization efficiency of human microbiota in germ-free (GF) mice, and the differential effects of gavage and lower gut transplantation on colonization are still unclear. In this study, we explored the reproducibility of recipient-to-donor gut microbiota community structure and function under different transplantation routes and the differences in microbial colonization between recipients via gavage (GT_mice group) and the lower gut (LGT_mice group). High-throughput sequencing of the metagenome was performed on the feces of each subject, and the composition of mi-crobiome of each group was analyzed. As expected, the introduction of human fecal microbiota into GF mice via lower gut trans-plantation had a high transfer efficiency, which was evident from the similar species community structure to the donor (Adonis R2, LGT_mice group – Donor group = 0.71396; GT_mice group – Donor group = 0.774095) and the higher bacterial colonization rate. The findings provide unique insights into improving the accuracy of constructing humanized microbiota transplantation models, aiding our understanding of the relationships between the human gut microbiota and disease.
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