Full Text:
<1653>
Summary: 
<25>
CLC number:
On-line Access: 2025-11-19
Received: 2024-10-14
Revision Accepted: 2025-01-02
Crosschecked: 2025-11-19
Cited: 0
Clicked: 1670
Citations: Bibtex RefMan EndNote GB/T7714
Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols
| ||||||||||||||
Pengfei LI, Lin CHEN, Wei YUAN, Xingqiang LI, Xuesong FENG. Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols[J]. Journal of Zhejiang University Science B, 2025, 26(11): 1132-1136.
@article{title="Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols",
author="Pengfei LI, Lin CHEN, Wei YUAN, Xingqiang LI, Xuesong FENG",
journal="Journal of Zhejiang University Science B",
volume="26",
number="11",
pages="1132-1136",
year="2025",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2400516"
}
%0 Journal Article
%T Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols
%A Pengfei LI
%A Lin CHEN
%A Wei YUAN
%A Xingqiang LI
%A Xuesong FENG
%J Journal of Zhejiang University SCIENCE B
%V 26
%N 11
%P 1132-1136
%@ 1673-1581
%D 2025
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2400516
TY - JOUR
T1 - Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols
A1 - Pengfei LI
A1 - Lin CHEN
A1 - Wei YUAN
A1 - Xingqiang LI
A1 - Xuesong FENG
J0 - Journal of Zhejiang University Science B
VL - 26
IS - 11
SP - 1132
EP - 1136
%@ 1673-1581
Y1 - 2025
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2400516
Abstract: Macrophages derived from the human THP-1 cell line have been widely used as substitutes for primary macrophages in various macrophage-related studies. However, difficulties still exist in establishing THP-1 macrophage models. This research presents techniques for generating different phenotypes of activated macrophages derived from THP-1 cells by introducing specific stimuli and provides some potential markers to confirm each type of activated macrophage. It is hoped to provide novel and useful methods for scientific research and to help researchers explore this field more intuitively and effectively.
[1]BaoYZ, WangGL, LiHJ, 2024. Approaches for studying human macrophages. Trends Immunol, 45(4):237-247.
[2]ChanputW, MesJJ, WichersHJ, 2014. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol, 23(1):37-45.
[3]HuangC, LewisC, BorgNA, et al., 2018. Proteomic identification of interferon-induced proteins with tetratricopeptide repeats as markers of M1 macrophage polarization. J Proteome Res, 17(4):1485-1499.
[4]HuangX, LiY, FuMG, et al., 2018. Polarizing macrophages in vitro. In: Rousselet G (Ed.), Macrophages: Methods and Protocols. Springer, New York, p.119-126.
[5]LepurA, CarlssonMC, NovakR, et al., 2012. Galectin-3 endocytosis by carbohydrate independent and dependent pathways in different macrophage like cell types. Biochim Biophys Acta Gen Subj, 1820(7):804-818.
[6]LiPF, HaoZF, WuJY, et al., 2021. Comparative proteomic analysis of polarized human THP-1 and mouse RAW264.7 macrophages. Front Immunol, 12:700009.
[7]LiPF, MaC, LiJ, et al., 2022. Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 23(5):407-422.
[8]LiuTZ, HuangT, LiJJ, et al., 2023. Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA. PLoS ONE, 18(7):e0286056.
[9]MarrufoAM, Flores-MirelesAL, 2024. Macrophage fate: to kill or not to kill? Infect Immun, 92(9):e0047623.
[10]SapudomJ, MohamedWKE, Garcia-SabatéA, et al., 2020. Collagen fibril density modulates macrophage activation and cellular functions during tissue repair. Bioengineering (Basel), 7(2):33.
ORCID: 
Open peer comments: Debate/Discuss/Question/Opinion
<1>