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Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila
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Lingqi YU1, Danfeng WANG1,3, Xuanhao CHEN1, Jiayu XIE4, Dongjing WEN1, Yi ZHANG1, Lirong SHEN5, Wenfeng CHEN1,2, Zhenxing LIU6, Yufeng YANG1,2. Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila",
author="Lingqi YU1, Danfeng WANG1,3, Xuanhao CHEN1, Jiayu XIE4, Dongjing WEN1, Yi ZHANG1, Lirong SHEN5, Wenfeng CHEN1,2, Zhenxing LIU6, Yufeng YANG1,2",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2400624"
}
%0 Journal Article
%T Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila
%A Lingqi YU1
%A Danfeng WANG1
%A 3
%A Xuanhao CHEN1
%A Jiayu XIE4
%A Dongjing WEN1
%A Yi ZHANG1
%A Lirong SHEN5
%A Wenfeng CHEN1
%A 2
%A Zhenxing LIU6
%A Yufeng YANG1
%A 2
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2400624
TY - JOUR
T1 - Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila
A1 - Lingqi YU1
A1 - Danfeng WANG1
A1 - 3
A1 - Xuanhao CHEN1
A1 - Jiayu XIE4
A1 - Dongjing WEN1
A1 - Yi ZHANG1
A1 - Lirong SHEN5
A1 - Wenfeng CHEN1
A1 - 2
A1 - Zhenxing LIU6
A1 - Yufeng YANG1
A1 - 2
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2400624
Abstract: Royal jelly (RJ), secreted by the hypopharyngeal and mandibular glands of young worker bees, is rich in proteins, 80%-90% of which are major royal jelly proteins (MRJPs). While MRJPs from RJ have been shown to exhibit specific biological functions and are also expressed in neuronal cells, their roles in vivo remain poorly understood. The aim of this study was to elucidate the functional roles of individual MRJPs (MRJP1-9) in vivo by ectopically expressing them in Drosophila neurons in a binary expression system using fluorescent proteins as controls. Transcriptome sequencing revealed that although MRJP1-9 share similar tertiary structures, their overexpression affects distinct gene sets. MRJP1, MRJP2, MRJP3, MRJP5, and MRJP7 induced more differentially expressed genes, while MRJP4, MRJP6, MRJP8, and MRJP9 induced fewer such genes. Weighted gene co-expression network analysis (WGCNA) and gene set enrichment analysis (GSEA) revealed that MRJP1, MRJP2, MRJP3, MRJP5, and MRJP7 regulate overlapping gene sets, including an estradiol-responsive set, and activate cell proliferation pathways. MRJP6 lacked any significant gene set enrichment, while MRJP8 and MRJP9 modulated similar sets. Notably, the neuronal-specific overexpression of MRJP1, MRJP2, MRJP3, and MRJP5 in Drosophila showed activated cell proliferation-related pathways and increased body size, highlighting their functional diversity and context-dependent effects. These findings expand our understanding of the functional roles of MRJPs and provide a foundation for further exploring their biological significance in honeybees and beyond.
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