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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.9 P.730-735


Age-dependent expression of forkhead box O proteins in the duodenum of rats

Author(s):  Pan Huang, Zhen-qi Zhou, Rui-hua Huang, Bo Zhou, Quan-wei Wei, Fang-xiong Shi

Affiliation(s):  College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   fxshi@njau.edu.cn

Key Words:  O subfamily of forkhead box (FoxO), Age-dependence, Duodenum, Rat

Pan Huang, Zhen-qi Zhou, Rui-hua Huang, Bo Zhou, Quan-wei Wei, Fang-xiong Shi. Age-dependent expression of forkhead box O proteins in the duodenum of rats[J]. Journal of Zhejiang University Science B, 2011, 12(9): 730-735.

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author="Pan Huang, Zhen-qi Zhou, Rui-hua Huang, Bo Zhou, Quan-wei Wei, Fang-xiong Shi",
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publisher="Zhejiang University Press & Springer",

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%T Age-dependent expression of forkhead box O proteins in the duodenum of rats
%A Pan Huang
%A Zhen-qi Zhou
%A Rui-hua Huang
%A Bo Zhou
%A Quan-wei Wei
%A Fang-xiong Shi
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 9
%P 730-735
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000298

T1 - Age-dependent expression of forkhead box O proteins in the duodenum of rats
A1 - Pan Huang
A1 - Zhen-qi Zhou
A1 - Rui-hua Huang
A1 - Bo Zhou
A1 - Quan-wei Wei
A1 - Fang-xiong Shi
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 9
SP - 730
EP - 735
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000298

The o subfamily of forkhead box (FoxO) proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B (IGF-1/PI3K/PKB) signal pathway. The objective of the present study was to examine the expressions of three members of FoxO proteins, FoxO1, FoxO3a, and FoxO4 in the duodenum of Sprague-Dawley rats at different ages. The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner. At Day 21, there were no detectable localization and expression of FoxO4 in the duodenum, while, at Months 2 and 6, localization and expression of FoxO4 were distinct. In addition, FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats, but was not detectable in the same area in 6-month-old rats. Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2- and 6-month marks, it was still not detectable at Day 21. Besides, FoxO1 was not detectable in all parts and stages. Taken together, our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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