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Journal of Zhejiang University SCIENCE A 2001 Vol.2 No.1 P.94-99

http://doi.org/10.1631/jzus.2001.0094


MICROSATELLITE ALTERATION AND ITS CHARACTERISTICS IN COLORECTAL CARCINOMA


Author(s):  LAI Mao-de, ZHANG Yu-wei, GAO Yu-tong, HE Chao

Affiliation(s):  Department of Pathology, Medical College, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   LMD@sun.zju.edu.cn

Key Words:  microsatellite instability, replication error, colorectal carcinoma


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LAI Mao-de, ZHANG Yu-wei, GAO Yu-tong, HE Chao. MICROSATELLITE ALTERATION AND ITS CHARACTERISTICS IN COLORECTAL CARCINOMA[J]. Journal of Zhejiang University Science A, 2001, 2(1): 94-99.

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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2001.0094"
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%A LAI Mao-de
%A ZHANG Yu-wei
%A GAO Yu-tong
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A1 - LAI Mao-de
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A1 - HE Chao
J0 - Journal of Zhejiang University Science A
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DOI - 10.1631/jzus.2001.0094


Abstract: 
Objective: To determine the role of microsatellite alterations in carcinogenesis of colorectal carcinoma (CRC). Methods: Alterations of 10 microsatellite loci from 5 different chromosomes were detected in 92 colorectal cancers and their paired normal mucosa by PCR, denatured polyacrylamide gel electrophoresis and silver staining. Associations of microsatellite alterations with clinopathologic parameters were statistically clarified.Results: Alterations of microsatellite were classified into microsatellite instability type I, type II and loss of heterozygosity (LOH). The carcinoma with ≥30% loci microsatellite alterations was defined as replication error(RER) positive tumors. Of 92 cases, 14 were RER+. Microsatellite alterations of P53(1) and D18S363 loci (64.29%) was most commonly identified in the RER+ tumors. RER+ were more commonly seen in poorly differentiated carcinomas and tended to occur in mucoid carcinomas. The type of microsatellite alterations varied in different histological types of CRC. Conclusions: Microsatellite alteration is a common molecular event in CRC. Different microsatellite loci showed various biologic significance. P53(1) and D18S363 should be essentially detected loci that can show the RER status of tumors.

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Reference

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