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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.2 P.221-227

http://doi.org/10.1631/jzus.2003.0221


Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma


Author(s):  PENG Jia-ping, ZHENG Shu, XIAO Zuo-xiang, ZHANG Su-zhan

Affiliation(s):  Cancer Institute, College of Medicine, Zhejiang University, Hangzhou, 310009, China

Corresponding email(s):   Zhengshu@mail.hz.zj.cn

Key Words:  Hepatocellular carcinoma, Nitric oxide synthase, Angiogenesis, Bcl-2, Flow cytometric analyse


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PENG Jia-ping, ZHENG Shu, XIAO Zuo-xiang, ZHANG Su-zhan. Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma[J]. Journal of Zhejiang University Science A, 2003, 4(2): 221-227.

@article{title="Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma",
author="PENG Jia-ping, ZHENG Shu, XIAO Zuo-xiang, ZHANG Su-zhan",
journal="Journal of Zhejiang University Science A",
volume="4",
number="2",
pages="221-227",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0221"
}

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%T Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma
%A PENG Jia-ping
%A ZHENG Shu
%A XIAO Zuo-xiang
%A ZHANG Su-zhan
%J Journal of Zhejiang University SCIENCE A
%V 4
%N 2
%P 221-227
%@ 1869-1951
%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0221

TY - JOUR
T1 - Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma
A1 - PENG Jia-ping
A1 - ZHENG Shu
A1 - XIAO Zuo-xiang
A1 - ZHANG Su-zhan
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 2
SP - 221
EP - 227
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0221


Abstract: 
In this study, we examined the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) by immunohistochemical staining in 76 tissue sections collected from hepatocellular carcinoma (HCC) patients undergoing hepatectomy. Microvascular density (MVD) was determined by counting endothelial cells immunostained using anti-CD34 antibody. We performed DNA-flow cytometric analyses to elucidate the impact of iNOS and VEGF expression on the cell cycle of HCC. Most of the HCC cells that invaded stroma were markedly immunostained by iNOS antibody. The iNOS stain intensity of the liver tissue close to the tumor edge was stronger than that of HCC tissue, and the strongest was the hepatocytes closer to the tumor tissue. However, iNOS expression in 10 normal hepatic samples was undetectable. VEGF positive expression ratio was 84.8% in iNOS positive expression cases, and the ratio was 35.3% in negative cases. There was significant correlation (P=0.000) between iNOS and VEGF expression. Moreover, iNOS expression was significantly associated with bcl-2 and MVD, but without p53 expression. DNA-flow cytometric analyses showed that combined expression of iNOS and VEGF had significant impact on the cell cycle in HCC. PI (Proliferating Index) and SPF (S-phase fraction) in the combined positive expression of iNOS and VEGF group was significantly higher than that in the combined negative group. The present findings suggested that iNOS expression was significantly associated with angiogenesis, bcl-2 and cell proliferation of HCC.

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