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Received: 2016-03-04

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Crosschecked: 2016-11-07

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Qian-nan Diao


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.12 P.916-930


Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling

Author(s):  Qian-nan Diao, Yong-jun Song, Dong-mei Shi, Hong-yan Qi

Affiliation(s):  Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Key Laboratory of Protected Horticulture of Ministry of Education and Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   hyqiaaa@126.com

Key Words:  Antioxidant enzymes, Chilling tolerance, Hydrogen peroxide, Nitric oxide, Spermidine, Tomato

Qian-nan Diao, Yong-jun Song, Dong-mei Shi, Hong-yan Qi. Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling[J]. Journal of Zhejiang University Science B, 2016, 17(12): 916-930.

@article{title="Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling",
author="Qian-nan Diao, Yong-jun Song, Dong-mei Shi, Hong-yan Qi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling
%A Qian-nan Diao
%A Yong-jun Song
%A Dong-mei Shi
%A Hong-yan Qi
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 12
%P 916-930
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600102

T1 - Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling
A1 - Qian-nan Diao
A1 - Yong-jun Song
A1 - Dong-mei Shi
A1 - Hong-yan Qi
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 12
SP - 916
EP - 930
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600102

Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato’s response to chilling stress.


方法:通过检测氧合血红蛋白(HbO2)向高铁血红蛋白(metHb)的转化进行NO含量测定;通过与NO特异性荧光探针(DAF-FM DA)结合检测NO释放量(图1和2)。超氧化物歧化酶(SOD)活性根据其抑制氮蓝四唑(NBT)在光下的还原作用测定;过氧化物酶(POD)活性通过测定酶提取液与愈创木酚、过氧化氢(H2O2)的混合物的吸光度确定;过氧化氢酶(CAT)活性根据H2O2在240 nm波长下的降解能力来测定;抗坏血酸过氧化物酶(APX)活性的测定参照Nakano和Asada(1981)的方法在波长290 nm下测定(图6和7)。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[99]List of electronic supplementary materials

[100]Fig. S1 Effects of exogenous Put and Spd on leNR and leNOS1 relative expression in the leaves of tomato under chilling stress

[101]Table S1 Gene accession numbers and primer sequences of tomato NR and NOS1 in this study

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