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Received: 2003-10-29

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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.6 P.644-650


Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination

Author(s):  WU Xiao-feng, CAO Cui-ping

Affiliation(s):  Lab of Gene Engineering, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   wuxiaofeng@zju.edu.cn

Key Words:  Gene targeting, Recombinant vector, Bombyx mori L.

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WU Xiao-feng, CAO Cui-ping. Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination[J]. Journal of Zhejiang University Science A, 2004, 5(6): 644-650.

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author="WU Xiao-feng, CAO Cui-ping",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination
%A WU Xiao-feng
%A CAO Cui-ping
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%DOI 10.1631/jzus.2004.0644

T1 - Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination
A1 - WU Xiao-feng
A1 - CAO Cui-ping
J0 - Journal of Zhejiang University Science A
VL - 5
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SP - 644
EP - 650
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.0644

The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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