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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.6 P.644-650

http://doi.org/10.1631/jzus.2004.0644


Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination


Author(s):  WU Xiao-feng, CAO Cui-ping

Affiliation(s):  Lab of Gene Engineering, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   wuxiaofeng@zju.edu.cn

Key Words:  Gene targeting, Recombinant vector, Bombyx mori L.


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WU Xiao-feng, CAO Cui-ping. Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination[J]. Journal of Zhejiang University Science A, 2004, 5(6): 644-650.

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Abstract: 
The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.

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