CLC number: S881.2
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 9
Clicked: 6358
WU Xiao-feng, CAO Cui-ping. Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination[J]. Journal of Zhejiang University Science A, 2004, 5(6): 644-650.
@article{title="Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination",
author="WU Xiao-feng, CAO Cui-ping",
journal="Journal of Zhejiang University Science A",
volume="5",
number="6",
pages="644-650",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0644"
}
%0 Journal Article
%T Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination
%A WU Xiao-feng
%A CAO Cui-ping
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 6
%P 644-650
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0644
TY - JOUR
T1 - Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination
A1 - WU Xiao-feng
A1 - CAO Cui-ping
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 6
SP - 644
EP - 650
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0644
Abstract: The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.
[1] Brunette, E., Stribling, R., Debs, R., 1992. Lipofectin does not require the removal of serum.Nucleic Acids Research,20:1151.
[2] Felgner, P.L., Gadek, T.R., Holm, M., Roman, R., Danielsen, M., 1987. Lipofectin: A highly efficient, lipid-mediated DNA-transfection procedure.Proc. Natl. Aca. Sci.USA,84:7413.
[3] Flipsen, J.T., Mans, R.M., Kleefsman, A.W., Knebel-Morsdorf, D., Valk, J.M., 1995. Deletion of the baculovirus ecdysteroid UDP-glucosyltransferase gene induces early degeneration of Malpighian tubules in infected insects.J. Virol.,69:4529-4532.
[4] Hammer, R.E., Pursel, V.G., Rexroad, C.E.Jr., Wall, R.J., Bolt, D.J., Ebert, K.M., Palmiter, R.D., Brinster, R.L., 1985. Production of transgenic rabbits, sheep and pigs by microinjection.Nature,315:680-683.
[5] Malone, R.W., Felgner, P.L., Verma, I.M., 1989. Cationic liposome-mediated RNA transfection.Proc. Natl. Aca. Sci. USA,86:60-77.
[6] McCreath, K.J., Howcroft, J., Campbell, K.H.S., Colman, A., Schnieke, A.E., Kind, A.J., 2000. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells.Nature,405:1066-1069.
[7] Nataraju, J., Kanda, T., Yukihiro, K., Chavancy, G., Tamura, T., Couble, P., 1996. Attempt at transgenesis of the silkworm (Bombyx moriL.) by egg-injection of foreign DNA.Appl. Entomol. Zool,31:587-596.
[8] Oreilly, D.R., Miller, L.K., 1990. Regulation of expression of a baculovirus ecdysteroid UDP-glucosyltransferase gene.J. Virol.,64:1321-1328.
[9] Rong, Y.K., Golic, S., Kent, G., 2000. Gene-targeting by homologous recombination inDrosophia.Science,288:81-84.
[10] Sporlein, B., Koop, H.U., 1991. Lipofectin: direct gene transfer to highly plants using cationic liposomes.Theor.Appl. Genet.,83:1.
[11] Suraokar, M., Bradley, A., 2000. Targeting sheep.Nature,405:1004-1005.
[12] Thompson, S., Clarke, A.R., Pow, A.M., Hooper, M.L., Melton, D.W., 1989. Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells.Cell,56:313-321.
[13] Toshiki, T., Chantal, T., Corinne, R., Toshio, K., Pierre, C., 2000. Germ line transformation of the silkwormBombyx moriL. using a piggyBac transposon-derieved vector.Nature Biotechnology,18:81-84.
[14] Wu, X.F., Kamei, K., Sato, H., Sato, S., Takano, R., Ichida, M., Mori, H., Hara, S., 2001. High-level expression of human acidic and basic fibroblast growth factors in silkworm,Bombyx moriL. using recombinant baculovirus. Protein Expression and Purification(USA),21(1):192-200.
[15] Wu, X.F., Xu, Y.X., Shen, G.X., Kamei, K., Takano, R., Hara, S., 2003. Surface plasmon resonance analysis to evaluate the importance of sulfate groups in heparin for the binding with human aFGF and bFGF.Journal of Zhejiang University SCIENCE,4(1):86-94.
[16] Wu, X.F., Cao, C.P., Xu, Y.X., Lu, X.M., 2004. Construction of a host range-expanded hybrid baculovirus of BmNPV and AcNPV, and knockout of cysteinase gene for more efficient expression.Science in China (B),34(2):156-164.
[17] Yamao, M., Katayama, N., Nakazawa, H., Yamakawa, M., Hayashi, Y., Hara, S., Kamei, K., Mori, H., 1999. Gene targeting in the silkworm by use of a baculovirus.Gene Development,13:511-516.
Open peer comments: Debate/Discuss/Question/Opinion
<1>